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Figure 4 | Frontiers in Zoology

Figure 4

From: Looking like Limulus? – Retinula axons and visual neuropils of the median and lateral eyes of scorpions

Figure 4

DiI and DiO labelling via median or median and lateral eyes (A, B, fluorescence microscope; C–D, CLSM). A, B, DiI-labelled first and second median eye neuropils. Arrowheads point to axons extending to arcuate body with varicosities after bifurcation, few axons terminating within the arcuate body. Note same annulus as seen in Cobalt fills (arrow). A, frontal view; B, sagittal view. Bars 200 μm. C, Specimen as in A, B, studied with CLSM. Frontal view. Bar 100 μm. DE, Combined DiO-labelled median (green) and DiI-labelled lateral (yellow) eye neuropils. Frontal view. Bars 50 μm. D, DiO-labelled first and second median eye neuropils (green). Note that DiO-stained cell bodies (arrows) indicate transcellular staining. E, DiI-labelled first and second lateral eye neuropils (yellow). Note that DiI-stained cell bodies (arrows) indicate transcellular staining. F, Combined image of DiO-labelled median (green) and DiI-labelled lateral (yellow) eye neuropils. Encircled region of second median and lateral eye neuropils with labelled R-cell axons of both median and lateral eyes. L1, first lateral eye visual neuropil; L2, second lateral eye visual neuropil; M1, first median eye visual neuropil; M2, second median eye visual neuropil; M/L2, region of L2 or M2 with labelled R-cell axons of both median and lateral eyes; MON, median eye optic nerve.

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