Figure 5From: Environmental monitoring using next generation sequencing: rapid identification of macroinvertebrate bioindicator speciesExperimental design and data analysis pipeline. The first half of the pipeline (in blue) shows the experimental set up, where species in samples were amplified individually (using morphology, PCR-RFLP and Sanger sequencing) and in bulk using 454 pyrosequencing. The second half of the pipeline (in orange) deals with the analysis of the sequences generated with 454 pyrosequencing.Back to article page