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Figure 3 | Frontiers in Zoology

Figure 3

From: Development in a naturally acidified environment: Na+/H+-exchanger 3-based proton secretion leads to CO2 tolerance in cephalopod embryos

Figure 3

Tissue specific expression levels of acid-base relevant genes. Agarose gel of PCR-amplified cDNAs (A) for NHE3 (primer pair: slNHE3F1/slNHE3R1, predicted product size: 168), RhP (primer pair: slRhPF1/slRhPR1, predicted product size: 179) H+-ATPase (subunit A) (primer pair: slHAF1/slHAR1, predicted product size: 161), Na+/K+-ATPase (primer pair: slNKAF1/slNKAR1, predicted product size: 83), Na+/HCO3- co-transporter (primer pair: slNBCF1/slNBCR1, predicted product size: 188) and the reference gene UBC (primer pair: slUCPF1/slUCPR1, predicted product size: 127). Relative expression of acid–base relevant genes in body (B) and yolk epithelium (C) homogenates along the period of 120 h. Different letters indicate differences between time points for each gene. Values represent mean (n = 3) ± SEM.

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Frontiers in Zoology

ISSN: 1742-9994