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Figure 2 | Frontiers in Zoology

Figure 2

From: Myogenesis of Malacostraca – the “egg-nauplius” concept revisited

Figure 2

Muscle ontogeny of Gonodactylaceus falcatus. a Macroscopic image of adult female. b-g Maximum intensity projections of confocal image stacks taken from whole mount fluorescent staining. Nuclear stain (SYTOX) is shown in blue; muscle stain (Myo16-C6) is shown in red (c-g). b blend projection of reconstructed nuclei with overlaying transparent image of ectodermal nuclei (grey). Dorsal view of Gf I caudal papilla. Mesoteloblasts (labeled for the right hemisegment: rMT1-rMT4) and two rows of mesodermal cells are reconstructed and highlighted in artificial colors alternating yellow and red. Mesoderm anlagen of more anterior segments show advanced proliferation which makes delineation of segments difficult. They are not highlighted. Mesoteloblasts form a ring in the caudal papilla. The dotted line marks the border between mesoteloblasts and mesodermal cells for the right hemisegment. c-g Ventral views of semaphoronts Gf I - Gf V. In e-g only the anterior left hemisegments are shown. c Gf I the external egg-nauplius morphology is apparent but the first maxilla bud is visible and the caudal papilla is elongate and flexed anteriorly. Intrinsic muscle precursor in antenna 1 is marked with an asterisk. d Gf II. st, st-2, a2-l1, a2-l2, md-l2, md-l3 and lmp are visible (a clear distinction between lmp-t1 and lmp-post cannot be made). Intrinsic muscle precursors of the first antenna appear (asterisk). e Gf III. a1-m1, mx2-l1, lmp-md and lmp-mx2 appear. Intrinsic muscle precursors of the second antenna appear (asterisk). f Gf IV. st-1, lmp-mx1, a1-m2, a2-m1, a2-m2 and mx2-l2 are shown. lmp-md is no longer visible. g Gf V. Muscle precursors are slightly enlarged and md-l1 has appeared. Abbreviations: Ol Optic lobes, Cp Caudal papilla, A1, A2, Md, Mx1, Mx2, T1 appendage anlagen. Scalebars are 1 cm in a, 50 μm in b, 100 μm in c-g.

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