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Figure 4 | Frontiers in Zoology

Figure 4

From: Myogenesis of Malacostraca – the “egg-nauplius” concept revisited

Figure 4

Muscle ontogeny of Neomysis integer. a Macroscopic image of adult. b Blend-projection, c-g maximum intensity projections of confocal image stacks taken from whole mount fluorescent staining. Nuclear stain (TOPRO-3): cyan (b), blue (d-h); muscle stain (anti- Myo16C6-Cy3): red (d-h); Muscle stain (phalloidin-ALEXA488): green (f, g), multiple artificial colors (h, i). b Early nauplioid larva. The telson anlage is marked with brackets. c Ventral view of the growth zone (as in Figure 2b and 3d). Mesoteloblasts and mesodermal cells form transverse rows instead of rings. d Ni I. Anterior portion of lmp (lmp-mx2) and lmp-d is visible. e Overview of same specimen. White rectangle marks the field shown in d. f and g show anterior left half of the larva. f Ni II. st, md-m and intrinsic musculature (asterisk) have appeared. g Ni III. st-1, st-2, md-l2, lmp-mx1, mx1-m1, mx2-m1 and mx2-m2 are seen. h Ni IV. Lateral view; lateral and medial precursors are reconstructed and assigned artificial colors white and yellow respectively, the remaining muscle signal is shown in red. Novel lateral precursors: a2-l1/2, a2-l3, md-l1, mx1-l1, mx1-l2, mx2-l and t1-l. Novel medial precursors: a2-m1, a2-m2, mx1-m2 and t1-m. i Ni V. Reconstructed precursors and precursor groups, as in Figure 3g. Novel lateral precursors: a2-l1, a2-l2, a2-l3 (derived from a2-l1/2). md-l2 is lost. Abbreviations: Ol optic lobes, A1, A2, Md, Mx1, Mx2, T1, appendage anlagen, T telson anlage. Scalebars are 2 mm in a, 100 μm in b, e, h and I, 50 μm in c, f and g, 25 μm in d.

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