Figure 5

Muscle ontogeny of Parhyale hawaiensis. a Macroscopic image of adult. c-g Maximum intensity projections of confocal image stacks taken from whole mount specimens. h and i blend projections. Nuclear stain (TOPRO-3): blue in b-g. Muscle staining (anti- Myo16C6-Cy3): red in b-g, multiple artificial colors in h; muscle stain (phalloidin-ALEXA488): green in b-f, multiple artificial colors in i. b Extended optical section showing anterior portion of lmp (Overview shown in c). c Ph I. Dorsal view of embryo expressing phalloidin-signal in the CNS and first myogenic signals. Ventral portions of CNS are partly blocked by yolk. d Magnification of cephalic region in Ph I, ventral view. The CNS is shown as well as muscle pioneers of st. e Magnification of c, only left body half, dorsal view, showing lmp and lmp-d. Thoracic segments are marked with dotted lines. f Ph II. st2, ppg, lmp-t1 and lmp-d are present. Midgut caeca muscle anlagen (mgc) are shown. g-i: ventral views of the anterior left embryonic region. g Ph III. Novel precursors: lb (labral muscle precursors), st-1, st-3, a2-l1, mx2/t1-l, a2-m1, md-m, mx1-m1, mx1-m2, mx2-m3 and t1-m. h and i Individual precursors or groups of precursors are reconstructed and assigned artificial colors as in Figure 4i. h Ph IV. Novel precursors: a2-l2, md-l1, md-l3/mx1-l, mx2-l, t1-l1 and t1-l2, and a2-m2. i Ph V. Additional precursors: md-l2, mx2-l1, mx2-l2, mx2-m1, mx2-m2 and mx2-m3. Abbreviations: A1, A2, Md, Mx1, Mx2, T1 appendage anlagen, T2, T3, T4, T5, T6, T8 thoracic tergite anlagen. Scalebars are 2 mm in a, 25 μm in b, 100 μm in c-i.