Figure 3

Using 4D-microscopy for detecting mandibular cell division pattern. (A) One level of the 4D-microscopy image stack from the beginning grid formation. The first transverse rows are already established. The light blue dots mark the cells E(1)1 in row E(1). The red dots above mark the cells E(0)p1. The dotted black lines mark the region of the prospective left and right head lobes. (B) Created SIMI°BioCell-model of cell arrangement of the ventral ectoderm of the embryo shown in figure A. The cells which are interesting to trace are color coded. Each genealogical region (row) has a distinct color (red: row E(1), blue: row E(0)p, light yellow: row E(0)ap, dark yellow: E(0)aa). The Arabic numbers sign out the different columns. (C) The embryo shown in A further developed (about 24 h). One level of the 4D-microscopy image stack from the ending of the time lapse recording. Because of the beginning morphogenesis of the limb buds it is impossible to reconstruct the cellular arrangement only by one slide. Most of the cells are not in focus. As in A the daughter cells of E(1)1 are marked with light blue dots, and the daughter cells of E(0)p1 with red dots. The circles indicate the early mandibular buds. (D) Created SIMI°BioCell-model of cell arrangement of the area shown in figure C. The traced cells are color coded as in B. Note that the genealogical unit E(1) (dark red) has its typical grid pattern. Within the unit E(2) (grey) it was not possible to trace all cells, some of them missing in this model.