Figure 1

Anatomical overview of the rhinophore. Neuroanatomy of the rhinophore: confocal images of sections labelled with an antibody to serotonin, fluophore-conjugated phalloidin and propidium iodide. A, B: Sagittal sections of the rhinophore at different planes. F-actin labelling with phalloidin (red) revealed the course of muscle fibres (M). Serotonin-immunoreactivity (green) is present in the rhinophore nerve (RN), the rhinophore ganglion (RG) and the glomeruli (GL). C, D, E, F: Sections labelled with phalloidin (green) and propidium iodide (red). C, D, E are cross sections at different planes at the positions indicated in F. In the upper part of the rhinophore glomeruli are situated beneath the epithelium. The rhinophore ganglion is situated at the basis of the groove. G: Nuclear labelling with propidium iodide (red) reveals the inner sensory epithelium (SE). Serotonin immunoreactivity (green) is present in the Glomeruli (GL). Scale bars = 500 μM (scale bar in A is also valid for B, in C is also valid for D and E).