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Table 1 Statistical analysis of contraction cycle durations in Tethya wilhelma Results of a Mann-Whitney U-test on differences between endogenous and influenced contraction cycle durations (Dcc) of T. wilhelma (each comparison within one experimental series).

From: Neuroactive substances specifically modulate rhythmic body contractions in the nerveless metazoon Tethya wilhelma (Demospongiae, Porifera)

Substance

Concentration

Figure

Dccendogenous [min]

Dccsubstance-exposed [min]

U-test

significant difference in Dcc?

Acetycholine

2.8 mM

2a

78.3 ± 5.3 (N = 9)

86.9 ± 2.3 (N = 9)

p < 0.001

yes

Nicotine

25 – 50 μM

2b

101.3 ± 8.6 (N = 4)

111.3 ± 10.6 (N = 4)

p > 0.01

no

Caffeine

515 μM

3a

93.1 ± 7.6 (N = 6)

85.3 ± 7.3 (N = 6)

p > 0.01

no

 

515 μM

3b

105.0 ± 7.7 (N = 5)

44.12 ± 21.0 (N = 5)

p < 0.01

yes

 

515 μM

3c

85.5 ± 19.3 (N = 14)

52.5 ± 17.2 (N = 18)

p < 0.001

yes

 

257 μM

3d

113.3 ± 34.6 (N = 9)

78.8 ± 23.5 (N =)

p < 0.01

yes

Glycin

1.3 mM

4a

105.5 ± 23.5 (N = 6)

54.2 ± 9.1 (N = 14)

p < 0.001

yes

 

1.3 mM

4b

93.0 ± 10.7 (N = 9)

42.7 ± 5.5 (N = 10)

p < 0.001

yes

Adrenaline

44 μM

5a

88.7 ± 3.4 (N = 7)

154.8 ± 58.7 (N = 4)

p < 0.01

yes

 

22 μM

5b

88.5 ± 7.9 (N = 8)

109.8 ± 6.0 (N = 2)

n.d.a

n.d.a

Serotonine

260 – 520 μM

5d

115.3 ± 14.0 (N = 5)

96.3 ± 10.0 (N = 5)

p > 0.01

no

 

65 μM

5c

105.0 ± 8.8 (N = 5)

102.9 ± 2.8 (N = 4)

p > 0.01

no

(by NOC-12)

5.7 μM

6a

75.8 ± 13.0 (N = 8)

116.0 ± 23.8 (N = 8)

p < 0.01

yes

 

22.7 μM

6b

95.0 ± 9.4 (N = 6)

238.0 ± 58.2 (N = 5)

p < 0.01

yes

cAMP

288 mM

7a

108.0 ± 11.8 (N = 4)

83.3 ± 5.3 (N = 2)

n.d.a

n.d.a

 

288 mM

7b

105.5 ± 23.5 (N = 6)

73.5 ± 3.5 (N = 2)

n.d.a

n.d.a

  1. a not determined, not enough data available