Figure 6

A, B: Immunolocalization of synapsin (SYNORF 1; green) in the ventral nerve centre of adult Sagitta setosa combined with a nuclear marker (red); higher magnification of the specimen shown in Fig. 5 (A, B are black-white inverted). Overlying the pattern of microcompartments, synapsin immunolabelling is particularly strong in a lateral longitudinal stripe on both sides of the neuropil whereas a narrow medial stripe is devoid of labelling. B shows a slightly more ventral focus level of the specimen shown in A, and the microcompartments are not visible in this focus level. Scale bars: 50 μm C, D: peripheral part of the right lateral soma area of the nerve centre (ventral view) labelled with a nuclear dye (images are black-white inverted). The small neurons are arranged in transverse rows (arrows). Note the large cell bodies which are arranged in regular intervals and display only weakly labelled nuclei (asteriks). E, F, G: imunolocalization of RFamide (green) in the ventral nerve centre of Sagitta setosa (ventral views). Individually identifiable neurons are encircled (E, F) or labelled with letters (G). E is a double-labelled specimen combining RFimmunolcalization with synapsin immunohistochemistry (red). F, G are double-labelled specimen combining RFimmunolcalization with a nuclear marker (red). Abbreviations identifying the longitudinal bundles: IB intermediate bundle, LB lateral bundle, MB medial bundle. Scale bars: 50 μm (E,F), 25 μm (G).