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Figure 2 | Frontiers in Zoology

Figure 2

From: Clouded leopard phylogeny revisited: support for species recognition and population division between Borneo and Sumatra

Figure 2

Phylogenetic relationships among clouded leopards from mtDNA haplotypes and a Bayesian analysis of microsatellite genotypes. (A) Phylogenetic relationships based on minimum evolution (ME) among the clouded leopard mtDNA haplotypes from the concatenated 900 bp mitochondrial sequences comprising Cyt-b (286 bp), ATPase- 8 (186 bp) and control region (426 bp) gene fragments. Panthera samples and Felis catus samples were taken as outgroups. Trees constructed with PAUP [56] obtained under maximum parsimony (MP) and maximum likelihood (ML) criteria have identical topologies. Numbers above the branches represent bootstrap support (1000 replicates) for each three methods (ME/MP/ML); only those with > 80 % are shown. Numbers in parentheses represent the number of individuals sharing the same haplotype. We used Kimura 2-parameter distance with neighbor-joining (NJ) algorithm followed by tree-bisection reconnection branch-swapping procedure (TBR) for the ME analysis. MP trees were constructed using a heuristic search, with a random addition of taxa and TBR branch swapping. The ML approach was performed using a HKY85 model [60]. Haplotype codes are shown in Table 1. NEB 1 – 5, DIB 1 and 2, and outgroups have been described previously [15]. * Sequences of only two mtDNA genes (ATPase-8 and Cyt b) were included. (B) Greyscale bars are from the Bayesian admixture analysis of the microsatellite analysis. Different colours stand for different genetic groups. ID codes in are shown in Table 1.

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