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Table 1 Summary of parameters for qPCR genes studied.

From: Phasing of muscle gene expression with fasting-induced recovery growth in Atlantic salmon

Gene

Primer 5'-3'

Prod. size (bp)

Tm (°C)

E (%)

R2

Accession number

IGF-I

f:CCTGTTCGCTAAATCTCACTTC

226

80.3

100.3

0.998

EF432852

 

r:TACAGCACATCGCACTCTTGA

     

Myogenin

f:GTGGAGATCCTGAGGAGTGC

146

84.5

95

0.993

DQ294029

 

r:CTCACTCGACGACGAGACC

     

MyoD1a

f;CCAAATAGTTCCAGACGCAAG

104

79.8

102.1

0.999

AJ557148

 

r:ACAGCGGGACAGGCAGAGG

     

MEF2A

f:ACCGGCTACAACACCGAGTA

121

84.1

92.5

0.994

DY713536

 

r:CCTGGCCCAGTTGATGTT

     

MuRF1

f:AGGCGGGATCAGAGCTAAC

229

87.2

103.7

0.998

DN165465

 

r:CGACCATTCCAAAGTCCATC

     

MAFbx

f:AAAGGAAGCACTAAAGAGCGTC

137

83.6

97.2

0.996

DN165813

 

r:CTGGGACTTGGCAATGAGC

     

Pkm

f:GTGACCATGATGCACTCGATC

225

84.6

100.3

0.992

CK888371

 

r:GGACAGCGTGGGCGATAC

     

Pgk

f:CTCGGTGATGGGGCTTAGG

160

82.6

92.1

0.996

DN166327

 

r:TCATTGGTGGAGGCGACA

     

TALDO1

f:AGGTAGACGCCAGGCTTTC

125

82.4

99

0.994

EG912503

 

r:CCATGTTGAGGAGAGCTTGA

     

FBP1

f:TGGGATTGCCAACCTCTATG

153

81.3

96.9

0.996

EG896159

 

r:GCCCTCTCGTTCTCCTCTG

     

ALDOB

f:TCCGTGACCTCCTGTTCTCT

159

83.5

102.1

0.998

AF067796

 

r:CTGTGCCTTTGTCCACCTTA

     

CrebA

f:GGAGTCTGTTTCGCTAAGTCG

168

84.1

100.1

0.998

CU073780

 

r:CGTAGGACCGCTGGATGT

     

LPL

f:TGCTGGTAGCGGAGAAAGACAT

114

80.8

95.9

0.998

BI468076

 

r:CTGACCACCAGGAAGACACCAT

     

ADIPOQ

f:CCAGCCAGAAGGCAATGTAT

192

81.2

98.6

0.996

EG776984

 

r:CACCAACGACTCCACCTTC

     

MHC

f:GCACGCCACTGAAAAC

209

84.1

94.2

0.996

DN164736

 

r:CCTCAAGGTCGTCCACT

     

DGAT1

f:CATGCTGGAGGTGATG

222

80.1

96.5

0.998

DW564359

 

r:GGAAGCACAGTGTGACTGA

     

MLC2

f:TCAACTTCACCGTCTTCCTCAC

194

82.6

98.5

0.994

NM_001123716

 

r:GCCCACAGGTTCTTCATCTCC

     

EF1-α

fATCGGCTATGCCTGGTGAC

141

85

96.3

0.999

BG933853

 

r:ATGATGACCTGAGCGGTG

     

B-actin

fACCCAGATCATGTTTGAGACC

146

82.9

92.7

0.997

G933897

 

r:TCGTAGATGGGTACTGTGTGGG

     

RNA pol II

f:TACATGACCAAATATGAAAGG

157

84.5

94.6

0.998

BG936649

 

r:GATGATGGGGATCTTCCTGC

     

HPRT1

f:CCTCAAGAGCTACTGTAAT

255

80.8

93.6

0.997

EG866745

 

r:TCTGGAACCTCAAACCCTATG

     

18S

f:GCGTCCAACTTCTTA

189

85.7

95.3

0.998

AJ427629

 

r:CAATCCCCAATCCCTATC

     
  1. Forward and reverse primer sequences (5'-3'), amplicon product size in base pairs (bp), melting temperature of amplicon (Tm), PCR efficiency (E), regression analysis of plasmid dilution series (R2) and accession numbers for genes used in qPCR.