Figure 7

Metamorphosis and adult myoanatomy of Terebratalia transversa. (A-C) Overlay of CLSM maximum projection micrograph and light micrograph, anterior faces upward. F-actin is labelled in red and cell nuclei are labelled in blue. Scale bars equal 50 μm. (A) Larva during metamorphosis. A mosaic of larval and juvenile features are present including the pedicle (pe), the larval pedicle muscles (pm), the first rudiments of the juvenile tentacle musculature (tm), one diductor (di), and the ventral pedicle adjustors (vpa). (B) Juvenile 5 days after metamorphosis, dorsal view with the remaining larval setae (se), the mantle margin muscles (mm), the tentacle muscles (tm), the adductors (ad), the musculature of the intestine (in), the diductors (di), the ventral pedicle adjustors (vpa), the dorsal pedicle adjustors (dpa), and the pedicle (pe). (C) Juvenile 5 days after metamorphosis, ventral view with the remaining larval setae (se), rudiments of the mantle margin muscles (mm), rudiments of the tentacle muscles (tm), the adductors (ad), the ventral pedicle adjustors (vpa), the diductors (di), the dorsal pedicle adjustors (dpa), and the pedicle (pe). (D) Reconstruction of the 3D arrangement of the juvenile musculature based on the CLSM dataset used in C showing the dorsal pedicle adjustors (red), the adductors (dark blue), the mantle margin muscles (light blue), and the tentacle muscles (magenta). The ventral pedicle adjustors (yellow) are ventrally connected to the diductors (green).