Figure 4

A. Diversity (PD) represented within each site when the entire dataset is utilized (black bars) and when tramp species are not included (yellow bars). The rank importance of the three most diverse sites changes dramatically when known introduced species are excluded. B. The same analysis as in A except completed on a 135 bp tiny-barcode fragment from the extreme 5' end of the barcode region. Absolute values change, but the rank importance of each site and the effect of knowing whether species are native or tramp remain the same. This is the fragment size originally produced by pyrosequencing technology, suggesting that environmental barcode metagenomics of this hyperdiverse taxa would yield predictions equivalent to the sequencing technology used here (one sequence: one specimen).