Figure 5
3rd part of a dorsal to ventral series of vibratome sections (100 μm) triple labeled for the nuclear counter marker (NUC; blue), synapsin immunoreactivity (SYN; red) and allatostatin-like immunoreactivity (AST; green) (A and C) and series of silver impregnated microtome sections (10 μm) of an equivalent horizon (B and D). A to D: Photomontages of 2 × 2 pictures per slice. Corresponding pictures of equivalent horizons are shown in A and B and further ventrally C and D. Roman numerals I/II, IV and V identify unstructured neuropils; numbers 5, 6, 10 and 9/11 identify cell clusters. Other abbreviations: Arrowhead, single ASTir neuron; AcN, accessory neuropil; HN, hemiellipsoid neuropil; LAN, lateral antenna I neuropil; mPC, medial protocerebrum; DO, deutocerebral organ; ON, olfactory neuropil (olfactory lobe); TM, terminal medulla. Scale bar = 500 μm.