- Open Access
Blood feeding patterns of mosquitoes: random or structured?
Frontiers in Zoology volume 7, Article number: 3 (2010)
The foraging behavior of blood-sucking arthropods is the defining biological event shaping the transmission cycle of vector-borne parasites. It is also a phenomenon that pertains to the realm of community ecology, since blood-feeding patterns of vectors can occur across a community of vertebrate hosts. Although great advances in knowledge of the genetic basis for blood-feeding choices have been reported for selected vector species, little is known about the role of community composition of vertebrate hosts in determining such patterns.
Methods & Results
Here, we present an analysis of feeding patterns of vectors across a variety of locations, looking at foraging patterns of communities of mosquitoes, across communities of hosts primarily comprised of mammals and birds. Using null models of species co-occurrence, which do not require ancillary information about host abundance, we found that blood-feeding patterns were aggregated in studies from multiple sites, but random in studies from a single site. This combination of results supports the idea that mosquito species in a community may rely primarily on host availability in a given landscape, and that contacts with specific hosts will be influenced more by the presence/absence of hosts than by innate mosquito choices. This observation stresses the importance of blood-feeding plasticity as a key trait explaining the emergence of many zoonotic mosquito transmitted diseases.
From an epidemiological perspective our observations support the idea that phenomena promoting synchronization of vectors and hosts can promote the emergence of vector-borne zoonotic diseases, as suggested by observations on the linkages between deforestation and the emergence of several human diseases.
One of the goals of evolutionary ecology is to understand the ecological niche of species as a product of changing environments and their evolutionary background [1, 2]. Patterns of similarity in resource use across species in a community led to the proposal of guilds as a conceptualization of the interactions of species within the same trophic level . One of the ways to understand patterns of resource use by consumers belonging to a given guild is to assume that genetic components influence feeding preferences, as proposed for mosquitoes [4, 5]. This approach is based in a conservative view of niches, the notion that the evolutionary history of a consumer taxon limits the resources it exploits [6, 7]. However, as thoroughly accounted by Southwood , the niche of a species is primarily shaped by the environment where its life history takes place. As shown by MacArthur , related species can forage across all the available and suitable resources, but will tend to specialize in space or time to forage, thus rendering co-existence possible through niche partitioning. Although well grounded in field observations, MacArthur's conclusions came from the observation of a group of organisms, warblers, whose life history keeps the same niche through development. However, a wide variety of organisms have niche shifts during their ontogenetic development, and the adaptive usage of different resources during the different life history stages can lead to ecological patterns that deviate from the expectation of boundaries in resource use. Mosquitoes are one of many groups that have major shifts in resource exploitation directly related to their holometabolism, by which they change from aquatic (pre-adult stages) to terrestrial (adult) habitats; they are of particular importance for their role as both vectors of pathogens and nuisance .
It is well known that for aquatic mosquito larvae, spatio-temporal heterogeneities in larval habitats play a key role in shaping the community structure [11, 12], with different degrees of co-existence regulated by the dynamics of the external environment, e.g., climatic variability [13–15]. Co-existence of mosquito species larvae is also supported by differences in traits related to foraging on different resources . However, for adults, mouthparts for blood sucking are similar enough to enable blood-feeding foraging across most vertebrate hosts [17, 18]. Thus, the extent to which blood-feeding patterns of mosquitoes are shaped by the availability of blood-feeding resources, i.e., vertebrate hosts, versus innate feeding choice specificity, is a relevant question. From an applied perspective, understanding adult mosquito blood-foraging patterns on a community of hosts is essential for the identification of species that could be involved in the transmission of vector-borne pathogens in nature (see Additional file 1: Biodiversity and zooprophylactic effects). In principle, zooprophylaxis has the potential to reduce human risk for vector-borne pathogen transmission in settings with increased vertebrate biodiversity (see Additional file 2: Zooprophylaxis and dilution effect: the same natural history seen with different glasses). Identification of reservoirs/amplifying hosts also can guide effective targeting of species to be managed (by culling or vaccination) for disease control .
Mosquito blood foraging can be studied by direct observation [20, 21], the use of baited traps  or by the analysis of blood contents in the mosquito gut . Blood in a mosquito gut can be identified using a range of tools, which have evolved through time. These approaches have benefited from advances in the fields of immunology and molecular biology. From techniques based on immunological reactions [24, 25] to PCR-based DNA identification [26, 27] mosquito feeding patterns can be tested against a set of suspected hosts and across different landscapes (see Additional file 3: Mosquito blood-feeding analysis: from precipitin tests to DNA fingerprinting). Traditional approaches to analyzing host choice by vectors include the computation of the host utilization rate [28, 29], which is the percent of bloodmeals belonging to a focal host species (used as a proportion in models); the forage ratio  which is the host utilization rate divided by the proportional abundance of the focal host species; and the feeding index which is defined as the proportion of feeds on one host with respect to another divided by the expected proportion of feeds on these two hosts based on factors affecting feeding, such as host abundance and size. Some of these indices require information on the composition of the vertebrate host community which is often very difficult to gather. The lack of information on host community composition renders impossible the testing for randomness in blood-feeding patterns of adult mosquitoes. However, the qualitative information produced by patterns of presence/absence of specific host utilization across different mosquito species in a given setting, or across habitats for a given mosquito species, can be used to study feeding patterns without information on the host community composition, since resulting patterns are similar to those studied in community ecology, especially those used to study rules of community assemblage . Specifically, the number of species pairs that never co-occur, or 'checkerboards' , can be used to test hypotheses about segregation in the blood-feeding patterns of a community of vectors through the use of null-model tests. The Additional file 4: A Primer of null model testing of blood-feeding patterns illustrates the logic behind null model testing of vector blood-feeding patterns.
Null-model tests are tools originally proposed to test the randomness in community assemblages . Nowadays, null-models are considered pattern-generating models based on randomization of ecological data or on random sampling from a known or arbitrary distribution . Here, we analyze presence/absence patterns using data from published studies on mosquito blood-feeding patterns. Null-models are used to test whether mosquito foraging occurs randomly, i.e., revealing no host-specificity, or if mosquitoes obtain bloodmeals only from certain host species, either with a strong segregation of host species choice or the aggregation around at least one host. Each hypothesis can be respectively supported by random, segregated patterns (some host species fed upon only by some mosquito species), and aggregated (same host species fed upon by all mosquito species).
We searched PubMed http://www.ncbi.nlm.nih.gov (1966 to October 2008), ISI Web of Science http://www.isiknowledge.com (1916 to October 2008), the American Journal of Tropical Medicine and Hygiene, AJTMH http://www.ajtmh.org (1921 to February 2009) and Journal of Medical Entomology, JME (1964 to January 2009) to identify field studies on blood-feeding patterns of mosquito communities across a range of hosts. We used the following key-words for our search: mosquito and blood or host and feeding or foraging. We did not search in journals not indexed in the above referred databases and limited our search to articles in English, French, Spanish, Portuguese, Italian, Vietnamese and Hebrew, the languages dominated by the authors. We discarded articles that were focused on only one mosquito species, studies that were focused on multiple bloodmeals without reference to the ecological context of mosquito sampling and studies where mosquito collection was done with cue-specific baits, i.e., animals. We kept studies showing results on the feeding patterns of a community of mosquito species on a community of vertebrate hosts, where samples came from resting boxes, aspirators, gravid and light traps, i.e., host-independent sampling methods. We did a more thorough search in the AJTMH and JME after realizing that most of the articles with all conditions for inclusion in our study came from these two journals. Some of the manuscripts had unidentified bloodmeal sources that were ignored for the analyses. We focused on mosquito communities because only at this level of complexity can comparisons be made in order to determine if there is a strong segregation (segregated pattern) of host species by vector species that could forage across a community of vertebrate hosts. Studies were also characterized as single or multiple site, depending on the adjacency of sampling sites.
Null-model analyses were performed using the C-score [35, 36]. The best alternative is the number of checkerboards, which is the count of pairs of species that never co-occur (feed) in an ensemble of host species . However, in some cases mosquito species can have some overlap in their feeding patterns though they share such overlap only for a small subset of potential hosts. To overcome this limitation the C-score was proposed, which is computed by the following equation:
Where, for the case of our analysis S i and S j are the total number of host species for mosquito species i and j, Q ij the number of times mosquito species i and j share a host, and R the total number of mosquito species. This metric measures species segregation but does not require perfect checkerboards . An additional reason to choose the C-score is the superior statistical qualities of this index that make it a more reliable indicator of co-occurrence patterns when compared with less flexible indexes like the checkerboards . The tools described here are also useful for studying patterns of blood feeding for individual species across habitats and hosts.
For the datasets we tested the null hypothesis that mosquito species are equally likely to forage on any host species. The null models were fixed-equiprobable, i.e., row sums of the original matrix are preserved and column values are the same. The latter means the following in the context of this research: when a new random dataset is generated, the probability of a mosquito species (rows) feeding on any of the hosts (columns) is the same, but the number of host species upon which it can feed is proportional to the number of host species on which it fed in the original dataset. Once the output of all simulations is obtained, which are the C-scores for each simulated dataset, a distribution is constructed and the significance of the C-score obtained for the original dataset is compared to this distribution of simulated indices, and inference is based on how extreme is the C-score calculated from the original dataset when compared to the distribution of simulated C-scores. When the C-score obtained from the data is statistically significantly larger than that of the simulations, it means that mosquito species have strict host-specific feeding (segregated) patterns; when it is not statistically different, it implies that feeding patterns are random, and when the C-score from the data is statistically significantly smaller than that from the simulations, it means that all mosquito species share at least one host species in their bloodmeals from the community of hosts, indicating an aggregated feeding pattern. We chose the fixed-equiprobable algorithm, because it provides a robust predictor [34, 35], and allows to test the null hypothesis of equal preference among host species. Simulations were carried out using the software Ecosim 7.0 . For the tests we used 5000 randomizations.
We found a total of 48 articles related to blood-feeding patterns in mosquitoes. A total of 19 articles had data on mosquito and vertebrate host communities, but only 12 fulfilled the criteria to be analyzed, since only these articles reported the blood contents of all engorged mosquito species studied (Table 1). We noticed that studies from the states of Florida [37–42] and Connecticut [43, 44] in the USA, sampled a geographically restricted set of sites, with similar communities of hosts, but reported mosquito host use for selected species separately. To investigate the impacts of these potential biases on the analyses, we performed individual analyses for both the individual reports and for the full datasets obtained by joining the data from all the different mosquito and host species reported for each state (i.e., Florida and Connecticut), raising the total number of analyzed studies to 14 (Table 1). More than 75% of the studies were done in the US. The regional bias of our study reflects the fact that most blood-feeding studies have tended to be narrowly focused on "main vectors". For example, several studies on blood feeding patterns have been carried out in Africa, Asia and Latin America, but only one from the latter fulfilled the selection criteria of including the vector community (Table 1), while the rest were focused on the "main vector". The most common mammal host species (Additional file 5: Table S1) were rabbits (81% of the studies with data on mammals), humans (75%) and horses (68%). For birds the most common across all studies specific to avian species (Additional file 6: Table S2) were: American Robin (100%), Gray Catbird, Northern Cardinal and Brown-headed Cowbird (each present in 83% of the studies). The most common mosquito species (Table 2) were Culex pipiens complex (47.37%), Aedes vexans (36.84%) and Anopheles quadrimaculatus (36.84%). We also found a great heterogeneity in the length of studies, ranging from a couple of weeks [26, 45], to seasonal (covering one year of mosquito season ), to longitudinal (spanning in some case as much as at least five years [37–42]), see Additional file 7: Table S3 for details on sampling frequency and study length.
Table 3 shows a total of 12 studies with data on vertebrate classes. Table 4 presents 7 studies with data on avian species, and Table 5 has results for 11 studies on mammalian species. These three tables present the results of the C-score null model tests. At the vertebrate class level, mosquitoes fed in an aggregated pattern (Table 3), with most species feeding either on mammal or avian species (Additional files 5 and 6: Table S1 and Table S2). In general, studies from multiple locations had observations that correspond to larger spatial scales, e.g. counties and states, or metropolitan areas , when compared to single locations. The feeding patterns from studies from multiple sites were primarily aggregated.
For birds (Table 4), the patterns were also primarily aggregated. The only study that showed random patterns  was also at a very local scale from a single site in the city of New York. A similar pattern of aggregation and randomness was observed for mammals (Table 5) and differences in patterns inferred with the C-score and the number of checkerboards likely arose from the absence of perfect checkerboards.
In summary, all results indicated that feeding patterns for the mosquito species and localities studied were aggregated when multiple sites were sampled, but random across available choices at the single, probably more local spatial scale. The above generalizations are hold for the more comprehensive analysis of the mosquito faunas of: (i) Florida, where an increase in sampled locations led to an aggregate pattern as opposed to the random pattern observed at the single location; and (ii) Connecticut, where an increase in the richness of mosquito and host species led to a similar aggregated pattern as the one observed for the split mosquito community sampled across multiple locations.
Our results show that in studies where data were gathered from multiple sites, mosquito species showed an aggregated feeding pattern which indicated that most mosquito species shared at least one host species. This aggregated pattern could arise from host choice based on host availability as reported elsewhere [48, 49]. For example, in the datasets we studied the most conspicuous example of aggregation around a given host was documented in Connecticut, USA , where all mosquito species fed on deer. Deer in New England, (which includes Connecticut) although not the most abundant vertebrate in terms of individuals, are a readily available source of blood to vectors . The latter has been shown for adult ticks, with deer size and perhaps the lack of host defensive, anti-biting behavior, given as possible explanations . Another complementary explanation may be the increased species richness in the meta-community of mosquitoes across multiple locations, as observed in Florida (Table S3), which is expected from the increase in the sampling of different mosquito habitats with multiple locations when compared to the coarser habitats of vertebrate hosts. For example, in Connecticut while deer are common in any natural area , the larval and resting habitats for different mosquito species are conditioned by vegetation and soil type .
By contrast to studies with multiple sites, studies with a single sampling location showed that feeding patterns did not deviate significantly from random, as can be expected given that restricted habitats offer limited resources . The result of aggregation of bloodmeals in at least one vertebrate class or one host species by the community of mosquitoes is suggestive in the sense that it supports the critical role that synchronization in the encounter of host and blood-feeding insects plays in mosquito foraging . This spatio-temporal co-occurrence is a more general requirement for any trophic interaction between a resource and a consumer . This synchronization could also explain the absence of dilution effects in urban environments , since species also get more crowded in patches of habitat following habitat transformation and fragmentation , which are not always associated with biodiversity loss . This finding contradicts the generalization that physiological factors are the major determinant of blood host choice by vectors, which postulates that close association with a narrow range of hosts would select for specialization in vector physiology that would limit the range of hosts that a species can exploit . While this may be the case for fleas  and kissing bugs  it is important to note that these groups exploit a unique resource throughout their life histories, unlike female mosquitoes that shift from exploitation of aquatic resources, where they are often very specialized in terms of habitat choice , to blood feeding from terrestrial hosts.
In mosquitoes it has been argued that some species have strong predilection for specific bloodmeal sources, the best example being Aedes aegypti and its preference for humans [59, 60]. However, blood-meal analyses for Ae. aegypti have shown that choices can be plastic, since bloodmeals can come from several species besides humans [60, 61]. Ae. albopictus, another major dengue vector shows plasticity with regard to blood foraging: in Thailand, mosquitoes sampled from endemic dengue villages had fed exclusively on humans ; in North America where mosquito contact with humans is less likely to occur (given the generalized anti-mosquito screening of houses), this species was catholic in its feeding repertoire, with bloodmeals coming from very diverse sources, including several mammal and avian species [62, 63]. In fact, a recent study found wildlife mammals infected with the dengue virus serotypes circulating in humans during several outbreaks in French Guiana, suggesting that dengue vectors feeding on infected humans can also feed on other mammal species . Examples are not restricted to culicines. In fact, Anopheles gambiae, the major malaria vector that has been suggested to be highly anthropophilic  and does have a strong preference for humans even when given other choices of blood hosts under controlled field settings, uses cows as its primary blood source in areas of Burkina Faso where humans, because of the widespread use of bednets, are not available as blood source . Thus, we propose that mosquito specialization in resource exploitation is likely to be primarily limited to pre-adult stages. Although differences in reproductive fitness of adult female mosquitoes fed over an array of potential hosts have been reported for some species , trade-offs in fitness performance may select for generalist behavior in blood-feeding patterns, or for alternative life history strategies, like autogeny, which is not uncommon in mosquitoes .
The randomness of host-feeding patterns by mosquitoes in single locations further reinforces the idea of non-specialization for blood sources in mosquitoes, especially when evaluated in conjunction with behavioral studies on host choices in the laboratory, and from field studies looking at seasonal changes in feeding patterns. Classical studies on mosquito blood-feeding behavior have shown that some mosquitoes can be opportunistic with regard to their feeding choices , and when differences have been observed they are related to the ease with which mosquitoes locate hosts [18, 49, 68], limitations in access to hosts [49, 66], or by a non-heritable behavioral conditioning . Patterns of blood host shift are also related to host abundance, as shown for Culex nigripalpus at subtropical latitudes , which fed on birds when they were the more abundant vertebrate. Aside from the effect of relative host abundance, the other major factor that can condition mosquito blood foraging is the defensive behavior of hosts [70–72]. Studies have found that defensive behavior can explain site selection for bites on a given host , and that differences in this behavior can be related to host age [73, 74] and size [20, 75]. The use of mechanical barriers such as bed nets [66, 76] also influence mosquito blood foraging, as can health or stress effects related to pathogen infection in the host . Thus, although more studies are required, especially of mosquito communities that comprise major vectors assumed to be highly anthropophilic, behavioral and environmental factors that are independent of genetic make-up could regulate mosquito blood foraging across a community of vertebrate hosts.
Directions for future research and implications
Our study has some limitations related to the scarce knowledge about mosquito community ecology, and heterogeneities in the sampling and design of the studies we analyzed and the analytical tool we used to study the feeding patterns: a) the variable sampling techniques for the collection of blood-fed mosquitoes(whose effects on biasing mosquito species composition can be very variable[37–42, 71]); b) the techniques for bloodmeal identification changed through time (following the development of molecular techniques); c) studies were geographically biased for North America; d) Lack of definition and quantification of the areas sampled (which precluded a formal definition of spatial scale); e) lack of definition and quantification of sample diversity to assess whether blood-fed mosquito species were representative of the whole community (we relied on the expert opinion expressed in the studies we analyzed) f) probabilities for host choice in the randomization algorithm were not weighted by the number of mosquitoes captured within a study that shared a given blood-meal type or by the abundance or biomass of potential hosts species. All these issues can be addressed as new studies focus in communities of mosquitoes, not only specific vectors. Also, it is necessary that ecological studies on mosquitoes more routinely incorporate the evaluation of species richness with well established quantitative methods, such as the extrapolation from species accumulation with sampling effort  and leave behind expert opinion as a criterion to support a representative sampling of mosquito diversity. Weighing can be easily implemented in the randomization algorithm of the null model tests, and very likely molecular tools for blood feeding analysis will evolve well beyond what is currently acknowledged as the "cutting edge".
Mosquito blood-feeding also has an additional layer of complexity, which is added by vector foraging heterogeneity on hosts belonging to the same species. For example, in a study of Ae. albopictus, 80% of human bloodmeals came from the same individual . This finding demonstrates one of the major limitations of more quantitative tools based on host abundance (foraging ratio, etc), namely the inherent heterogeneity of sources for vector-feeding across populations of hosts . This is an issue deserving more studies, taking advantage of molecular tools, like DNA fingerprinting [27, 63, 80]. This fact also leads to a possible mechanism linking host population size and feeding patterns, since larger populations have increased likelihoods of bearing stressed individuals , which are more likely to be bitten, primarily because of lack of defensive behaviors . These same patterns also arise in neglected and minority populations or endangered species  which further supports the need to look qualitatively at presence/absence of hosts, since mosquito foraging may not necessarily be a functional response of host density .
From an applied perspective, and especially in the context of zoonotic vector-borne diseases, the broad range of blood hosts that may be fed upon by mosquitoes is one of the underpinnings for disease emergence, as was early recognized by the scientific community studying arboviral transmission [28, 83, 84] and has long been recognized as a regulatory factor for malaria transmission (see Additional file 2). In the mosquito communities we analyzed, most mosquito species can feed on at least one common host species independently of their innate preferences. Synchronization of encounters between hosts and vectors can promote disease emergence by facilitating foraging across a community of pathogen-susceptible hosts. Anthropogenic changes, such as deforestation and forest fragmentation can increase mosquito dispersal and foraging across abundant hosts , a pattern observed in other vectors such as sandflies  and ticks . More generally, transmission control strategies such as insecticide treated nets that target host-vector contact have been the most successful at stopping the transmission of vector-borne diseases , demonstrating the importance of taking into account vector foraging . From a theoretical perspective the explanation for the coupling of foraging specialization in organisms with niche shifts deserves further inquiry. The evolution of a life history strategy with different degrees of foraging specialization across ontogenetic stages could explain some cases of species co-occurrence and can be one mechanism for speciation in mosquitoes.
Finally, our study highlights the potential of mosquitoes as a model system to study foraging in organisms with niche shifts during their life cycle, where several factors are still unknown, such as the effects of nectar feeding on blood foraging and pathogen development . It also illustrates the use of ecological theory and derived tools to further understanding of patterns that are of importance in studying and managing human diseases.
Levins R: Evolution in Changing Environments. Some theoretical explorations. 1968, Princeton: Princeton University Press
Schmalhausen II: Factors of evolution; the theory of stabilizing selection. 1949, Philadelphia: Blakiston Co
Root RB: Niche Exploitation Pattern of Blue-Gray Gnatcatcher. Ecological Monographs. 1967, 37: 317-350. 10.2307/1942327.
Kilpatrick AM, Kramer LD, Jones MJ, Marra PP, Daszak P, Fonseca DM: Genetic Influences on Mosquito Feeding Behavior and the Emergence of Zoonotic Pathogens. American Journal of Tropical Medicine and Hygiene. 2007, 77: 667-671.
Huang S, Hamer GL, Molaei G, Walker ED, Goldberg TL, Kitron UD, Andreadis TG: Genetic Variation Associated with Mammalian Feeding in Culex pipiens from a West Nile Virus Epidemic Region in Chicago, Illinois. Vector-Borne and Zoonotic Diseases. 2009, 9: 637-642. 10.1089/vbz.2008.0146.
Gould SJ, Lewontin RC: Spandrels of San-Marco and the Panglossian Paradigm - a Critique of the Adaptationist Program. Proceedings of the Royal Society of London Series B-Biological Sciences. 1979, 205: 581-598. 10.1098/rspb.1979.0086.
Brooks DR, Hoberg EP: How will global climate change affect parasite-host assemblages?. Trends in Parasitology. 2007, 23: 571-574. 10.1016/j.pt.2007.08.016.
Southwood TRE: Habitat, Templet for Ecological Strategies - Presidential-Address to British-Ecological-Society, 5 January 1977. Journal of Animal Ecology. 1977, 46: 337-365.
MacArthur RH: Population Ecology of Some Warblers of Northeastern Coniferous Forests. Ecology. 1958, 39: 599-619. 10.2307/1931600.
Edman JD, Day J, Walker ED: Vector-Host interplay - Factors affecting disease transmission. Ecology of mosquitoes: proceedings of a workshop. Edited by: Lounibos LP, Rey JR, Frank JH. 1985, Vero Beach: Florida Medical Entomology Laboratory, 273-285:
Juliano SA: Species interactions among larval mosquitoes: context dependence across habitat gradients. Annual Review of Entomology. 2009, 54: 37-56. 10.1146/annurev.ento.54.110807.090611.
Mogi M: Populations studies on mosquitoes in the rice field area of Nagasaki, Japan, especially on Culex tritaeniorhynchus. Tropical Medicine. 1978, 20: 173-263.
Kirby MJ, Lindsay SW: Effect of temperature and inter-specific competition on the development and survival of Anopheles gambiae sensu stricto and An. arabiensis larvae. Acta Tropica. 2009, 109: 118-123. 10.1016/j.actatropica.2008.09.025.
Paaijmans KP, Huijben S, Githeko AK, Takken W: Competitive interactions between larvae of the malaria mosquitoes Anopheles arabiensis and Anopheles gambiae under semi-field conditions in western Kenya. Acta Tropica. 2009, 109: 124-130. 10.1016/j.actatropica.2008.07.010.
Minakawa N, Sonye G, Mogi M, Githeko A, Yan G: The effects of climatic factors on the distribution and abundance of malaria vectors in Kenya. Journal of Medical Entomology. 2002, 39: 833-841. 10.1603/0022-2585-39.6.833.
Merritt RW, Dadd RH, Walker ED: Feeding behavior, natural food, and nutritional relationships of larval mosquitoes. Annual Review of Entomology. 1992, 37: 349-376.
Hocking B: Blood-Sucking Behavior of Terrestrial Arthropods. Annual Review of Entomology. 1971, 16: 1-28. 10.1146/annurev.en.16.010171.000245.
Lehane M: The biology of blood-sucking in insects. 2005, Cambridge: Cambridge University Press, 2
Chaves LF, Hernandez MJ, Dobson AP, Pascual M: Sources and sinks: revisiting the criteria for identifying reservoirs for American cutaneous leishmaniasis. Trends in Parasitology. 2007, 23: 311-316. 10.1016/j.pt.2007.05.003.
Sota T, Hayamizu E, Mogi M: Distribution of Biting Culex tritaeniorhynchus (Diptera, Culicidae) among Pigs: Effects of Host Size and Behavior. Journal of Medical Entomology. 1991, 28: 428-433.
Fujito S, Buei K, Nakajima S, Ito S, Yoshida M, Sonada H, Nakamura H: Effect of the population density of Culex tritaeniorhynchus Giles on bloodsucking rates in cowsheds and pigpens in relation to its role in the epidemic of Japanese encephalitis. Japanese Journal of Sanitary Zoology. 1971, 22: 38-44.
Sullivan MF, Gould DJ, Maneechai S: Observations on host range and feeding preferences of Aedes albopictus (Skuse). Journal of Medical Entomology. 1971, 8: 713-716.
Kay BH, Boyd AM, Ryan PA, Hall RA: Mosquito feeding patterns and natural infection of vertebrates with Ross River and Barmah Forest viruses in Brisbane, Australia. American Journal of Tropical Medicine and Hygiene. 2007, 76: 417-423.
Tempelis CH, Washino RK: Host-Feeding Patterns of Culex tarsalis in Sacramento Valley California with Notes on Other Species. Journal of Medical Entomology. 1967, 4: 315-318.
Bull CG, King WV: The identification of the blood meal of mosquitoes by means of the precipitin test. American Journal of Tropical Medicine and Hygiene. 1923, 3: 491-496.
Apperson CS, Harrison BA, Unnasch TR, Hassan HK, Irby WS, Savage HM, Aspen SE, Watson DW, Rueda LM, Engber BR, Nasci RS: Host-feeding habits of Culex and other mosquitoes (Diptera: Culicidae) in the Borough of Queens in New York City, with characters and techniques for identification of Culex mosquitoes. Journal of Medical Entomology. 2002, 39: 777-785. 10.1603/0022-2585-39.5.777.
Scott TW, Githeko AK, Fleisher A, Harrington LC, Yan G: DNA profiling of human blood in anophelines from lowland and highland sites in western Kenya. American Journal of Tropical Medicine and Hygiene. 2006, 75: 231-237.
Hess AD, Hayes RO: Relative Potentials of Domestic Animals for Zooprophylaxis against Mosquito Vectors of Encephalitis. American Journal of Tropical Medicine and Hygiene. 1970, 19: 327-334.
Hess AD, Hayes RO, Tempelis CH: Use of forage ratio technique in mosquito host preference studies. Mosquito News. 1968, 28: 386-387.
Kay BH, Boreham PFL, Williams GM: Host preferences and feeding patterns of mosquitoes (Diptera:Culicidae) at Kowanyama, Cape York Peninsula, Northern Quensland. Bulletin of Entomological Research. 1979, 69: 441-457. 10.1017/S0007485300018952.
Brown JH: Macroecology. 1995, Chicago: The University of Chicago Press
Diamond JM: Assembly of species communities. Ecology and evolution of communities. Edited by: Cody ML, Diamond JM. 1975, Cambridge, MA, USA: Harvard University Press, 342-444.
Connor EF, Simberloff D: The Assembly of Species Communities - Chance or Competition. Ecology. 1979, 60: 1132-1140. 10.2307/1936961.
Gotelli NJ: Null model analysis of species co-occurrence patterns. Ecology. 2000, 81: 2606-2621.
Stone L, Roberts A: Competitive-Exclusion, or Species Aggregation - an Aid in Deciding. Oecologia. 1992, 91: 419-424. 10.1007/BF00317632.
Stone L, Roberts A: The Checkerboard Score and Species Distributions. Oecologia. 1990, 85: 74-79. 10.1007/BF00317345.
Edman JD: Host-Feeding Patterns of Florida Mosquitoes I. Aedes, Anopheles, Coquillettidia, Mansonia, and Psorophora. Journal of Medical Entomology. 1971, 8: 687-695.
Edman JD: Host-Feeding Patterns of Florida Mosquitoes (Diptera: Culicidae) VI. Culex (Melanoconion). Journal of Medical Entomology. 1979, 15: 521-525.
Edman JD: Host-Feeding Patterns of Florida Mosquitoes. IV. Deinocerites. Journal of Medical Entomology. 1974, 11: 105-107.
Edman JD: Host-Feeding Patterns of Florida Mosquitoes. III. Culex (Culex) and Culex (Neoculex). Journal of Medical Entomology. 1974, 11: 95-104.
Edman JD, Webber LA, Kale HW: Host-Feeding Patterns of Florida Mosquitoes II. Culiseta. Journal of Medical Entomology. 1972, 9: 429-434.
Edman JD, Haeger JS: Host-feeding patterns of Florida mosquitoes V. Wyeomyia. Journal of Medical Entomology. 1977, 14: 471-479.
Molaei G, Andreadis TA, Armstrong PM, Anderson JF, Vossbrinck CR: Host feeding patterns of Culex mosquitoes and West Nile virus transmission, northeastern United States. Emerging Infectious Diseases. 2006, 12: 468-474.
Molaei G, Andreadis TG, Armstrong PM, Diuk-Wasser M: Host-feeding patterns of potential mosquito vectors in Connecticut, U.S.A.: molecular analysis of bloodmeals from 23 species of Aedes, Anopheles, Culex, Coquillettidia, Psorophora, and Uranotaenia. Journal of Medical Entomology. 2008, 45: 1143-1151. 10.1603/0022-2585(2008)45[1143:HPOPMV]2.0.CO;2.
Fyodorova MV, Savage HM, Lopatina JV, Bulgakova TA, Ivanitsky AV, Platonova OV, Platonov AE: Evaluation of potential West Nile virus vectors in Volgograd region, Russia, 2003 (Diptera: Culicidae): Species composition, bloodmeal host utilization, and virus infection rates of mosquitoes. Journal of Medical Entomology. 2006, 43: 552-563. 10.1603/0022-2585(2006)43[552:EOPWNV]2.0.CO;2.
Forattini OP, Gomes AD, Natal D, Kakitani I, Marucci D: Feeding Preferences and Domiciliation of Culicidae Mosquitos in the Ribeira Valley, Sao Paulo State, Brazil, with Particular Reference to Aedes scapularis and Culex (Melanoconion). Revista De Saude Publica. 1989, 23: 9-19.
Hamer GL, Kitron UD, Goldberg TL, Brawn JD, Loss SR, Ruiz MO, Hayes DB, Walker ED: Host Selection by Culex pipiens Mosquitoes and West Nile Virus Amplification. American Journal of Tropical Medicine and Hygiene. 2009, 80: 268-278.
Edman JD, Taylor DJ: Culex nigripalpus - Seasonal Shift in Bird-Mammal Feeding Ratio in a Mosquito Vector of Human Encephalitis. Science. 1968, 161: 67-68. 10.1126/science.161.3836.67.
Edman JD, Spielman A: Blood-feeding by vectors: physiology, ecology, behavior, and vertebrate defense. The Arboviruses: Epidemiology and Ecology. Edited by: Monath TP. 1988, Boca Raton, FL. USA: CRC Press, 1: 153-189.
Wilson ML, Litwin TS, Gavin TA, Capkanis MC, Maclean DC, Spielman A: Host-Dependent Differences in Feeding and Reproduction of Ixodes dammini (Acari: Ixodidae). Journal of Medical Entomology. 1990, 27: 945-954.
Wilson ML, Telford SR, Piesman J, Spielman A: Reduced Abundance of Immature Ixodes dammini (Acari: Ixodidae) Following Elimination of Deer. Journal of Medical Entomology. 1988, 25: 224-228.
Diuk-Wasser MA, Brown HE, Andreadis TG, Fish D: Modeling the spatial distribution of mosquito vectors for West Nile virus in Connecticut, USA. Vector-Borne and Zoonotic Diseases. 2006, 6: 283-295. 10.1089/vbz.2006.6.283.
Murdoch WM, Briggs CJ, Nisbet RM: Consumer-Resource dynamics. 2003, Princeton: Princeton University Press
Loss SR, Hamer GL, Walker ED, Ruiz MO, Goldberg TL, Kitron UD, Brawn JD: Avian host community structure and prevalence of West Nile virus in Chicago, Illinois. Oecologia. 2009, 159: 415-424. 10.1007/s00442-008-1224-6.
Terborgh J, Lopez L, Nunez P, Rao M, Shahabuddin G, Orihuela G, Riveros M, Ascanio R, Adler GH, Lambert TD, Balbas L: Ecological meltdown in predator-free forest fragments. Science. 2001, 294: 1923-1926. 10.1126/science.1064397.
Perfecto I, Vandermeer J: Biodiversity conservation in tropical agroecosystems - A new conservation paradigm. Annals of the New York Academy of Sciences. 2008, 1134: 173-200. 10.1196/annals.1439.011.
Krasnov BR, Sarfati M, Arakelyan MS, Khokhlova IS, Burdelova NV, Degen AA: Host specificity and foraging efficiency in blood-sucking parasite: feeding patterns of the flea Parapulex chephrenis on two species of desert rodents. Parasitology Research. 2003, 90: 393-399. 10.1007/s00436-003-0873-y.
Lent H: Evolution of knowledge on carriers of chagas diseases 90 years after its discovery. Memorias Do Instituto Oswaldo Cruz. 1999, 94: 89-92.
Harrington LC, Edman JD, Scott TW: Why do female Aedes aegypti (Diptera: Culicidae) feed preferentially and frequently on human blood?. Journal of Medical Entomology. 2001, 38: 411-422. 10.1603/0022-2585-38.3.411.
Ponlawat A, Harrington LC: Blood Feeding Patterns of Aedes aegypti and Aedes albopictus in Thailand. Journal of Medical Entomology. 2005, 42: 844-849. 10.1603/0022-2585(2005)042[0844:BFPOAA]2.0.CO;2.
Tempelis CH, Hayes RO, Hess AD, Reeves WC: Blood-Feeding Habits of 4 Species of Mosquito Found in Hawaii. American Journal of Tropical Medicine and Hygiene. 1970, 19: 335-341.
Savage HM, Niebylski ML, Smith GC, Mitchell CJ, Craig GB: Host-Feeding Patterns of Aedes albopictus (Diptera: Culicidae) at a Temperate North American Site. Journal of Medical Entomology. 1993, 30: 27-34.
Richards SL, Ponnusamy L, Unnasch TR, Hassan HK, Apperson CS: Host-Feeding Patterns of Aedes albopictus (Diptera: Culicidae) in Relation to Availability of Human and Domestic Animals in Suburban Landscapes of Central North Carolina. Journal of Medical Entomology. 2006, 43: 543-551. 10.1603/0022-2585(2006)43[543:HPOAAD]2.0.CO;2.
Thoisy Bd, Lacoste V, Germain A, Munoz-Jordan J, Colon C, Mauffrey J-Fo, Delaval M, Catzeflis Fo, Kazanji M, Matheus S: Dengue Infection in Neotropical Forest Mammals. Vector-Borne and Zoonotic Diseases. 2009, 9: 157-170. 10.1089/vbz.2007.0280.
Killeen GF, McKenzie FE, Foy BD, Bøgh C, Beier JC: The availability of potential hosts as a determinant of feeding behaviours and malaria transmission by African mosquito populations. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2001, 95: 469-476. 10.1016/S0035-9203(01)90005-7.
Lefèvre T, Gouagna LC, Dabire KR, Elguero E, Fontenille D, Renaud F, Costantini C, Thomas F: Beyond nature and nurture: phenotypic plasticity in blood-feeding behavior of Anopheles gambiae s.s. when humans are not readily accessible. American Journal of Tropical Medicine and Hygiene. 2009, 81: 1023-1029. 10.4269/ajtmh.2009.09-0124.
Edman JD: Disease control through manipulation of vector-host interaction: some historical and evolutionary perspectives. Proceedings of a Symposium: The Role of vector-host interactions in disease tranmission. Edited by: Scott TW, Grumstrup-Scott J. 1988, Washington, D.C.: Entomological Society of America, 43-50.
Dekker T, Takken W, Braks MAH: Innate Preference for Host-Odor Blends Modulates Degree of Anthropophagy of Anopheles gambiae sensu lato (Diptera: Culicidae). Journal of Medical Entomology. 2001, 38: 868-871. 10.1603/0022-2585-38.6.868.
Mwandawiro C, Boots M, Tuno N, Suwonkerd W, Tsuda Y, Takagi M: Heterogeneity in the host preference of Japanese encephalitis vectors in Chiang Mai, northern Thailand. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2000, 94: 238-242. 10.1016/S0035-9203(00)90303-1.
Walker ED, Edman JD: The Influence of Host Defensive Behavior on Mosquito (Diptera, Culicidae) Biting Persistence. Journal of Medical Entomology. 1985, 22: 370-372.
Edman JD, Kale HW: Host Behavior: Its Influence on the Feeding Success of Mosquitoes. Annals of the Entomological Society of America. 1971, 64: 513-516.
Edman JD, Webber LA, Schmid AA: Effect of Host Defenses on Feeding Pattern of Culex nigripalpus When Offered a Choice of Blood Sources. Journal of Parasitology. 1974, 60: 874-883. 10.2307/3278923.
Walker ED, Edman JD: Feeding-Site Selection and Blood-Feeding Behavior of Aedes triseriatus (Diptera, Culicidae) on Rodent (Sciuridae) Hosts. Journal of Medical Entomology. 1985, 22: 287-294.
Day JF, Edman JD: Mosquito Engorgement on Normally Defensive Hosts Depends on Host Activity Patterns. Journal of Medical Entomology. 1984, 21: 732-740.
Port GR, Boreham PFL: The relationship of host size to feeding by mosquitoes of the Anopheles gambiae Giles complex (Diptera: Culicidae). Bulletin of Entomological Research. 1980, 70: 133-144. 10.1017/S0007485300009834.
Port GR, Boreham PFL: The Effect of Bed Nets on Feeding by Anopheles gambiae Giles (Diptera, Culicidae). Bulletin of Entomological Research. 1982, 72: 483-488. 10.1017/S0007485300013663.
Day JF, Ebert KM, Edman JD: Feeding Patterns of Mosquitoes (Diptera, Culicidae) Simultaneously Exposed to Malarious and Healthy Mice, Including a Method for Separating Blood Meals from Conspecific Hosts. Journal of Medical Entomology. 1983, 20: 120-127.
Colwell RK, Coddington JA: Estimating terrestrial biodiversity through extrapolation. Philosophical Transactions of the Royal Society of London Series-B Biological Sciences. 1994, 345: 101-118. 10.1098/rstb.1994.0091.
Woolhouse ME, Dye C, Etard JF, Smith T, Charlwood JD, Garnett GP, Hagan P, Hii JL, Ndhlovu PD, Quinnell RJ: Heterogeneities in the transmission of infectious agents: implications for the design of control programs. Proceedings National Academy of Sciences USA. 1997, 94: 338-342. 10.1073/pnas.94.1.338.
De Benedictis J, Chow-Shaffer E, Costero A, Clark GG, Edman JD, Scott TW: Identification of the people from whom engorged Aedes aegypti t ook blood meals in Florida, Puerto Rico, using polymerase chain reaction-based DNA profiling. American Journal of Tropical Medicine and Hygiene. 2003, 68: 437-446.
Himmelstein DU, Levins R, Woolhandler S: Beyond our means - patterns of variability of phisiological traits. International Journal of Health Services. 1990, 20: 115-124.
Kelly DW, Thompson CE: Epidemiology and optimal foraging: modelling the ideal free distribution of insect vectors. Parasitology. 2000, 120: 319-327. 10.1017/S0031182099005442.
Reeves WC: Mosquito vector and vertebrate host interaction. The key to maintenance of certain arboviruses. The Ecology and Physiology of Parasites. Edited by: Fallis AM. 1971, Toronto: University of Toronto Press, 223-230.
Kay BH: Rainbow's end? Thoughts on some viral encephalitides with emphasis on Murray Valley Enecephalitis. Ecology of mosquitoes: proceedings of a workshop. Edited by: Lounibos LP, Rey JR, Frank JH. 1985, Vero Beach: Florida Medical Entomology Laboratory, 287-292:
Renshaw M, Service MW, Birley MH: Host Finding, Feeding Patterns and Evidence for a Memorized Home-Range of the Mosquito Aedes cantans. Medical and Veterinary Entomology. 1994, 8: 187-193. 10.1111/j.1365-2915.1994.tb00162.x.
Chaves LF, Cohen JM, Pascual M, Wilson ML: Social Exclusion Modifies Climate and Deforestation Impacts on a Vector-Borne Disease. PLoS Neglected Tropical Diseases. 2008, 2: e176-10.1371/journal.pntd.0000176.
Allan BF, Keesing F, Ostfeld RS: Effect of forest fragmentation on Lyme disease risk. Conservation Biology. 2003, 17: 267-272. 10.1046/j.1523-1739.2003.01260.x.
Lengeler C: Insecticide-treated bed nets and curtains for preventing malaria. Cochrane Database Systemic Reviews. 2004, CD000363-
Sota T: Variable host selection by mosquitoes and the dynamics of mosquito-borne diseases. Japanese Journal of Sanitary Zoology. 1992, 43: 1-11.
Manda H, Gouagna LC, Nyandat E, Kabir EW, Jackson RR, Foster WA, Githure JI, Beier JC, Hassanali A: Discriminative feeding behaviour of Anopheles gambiae s.s. on endemic plants in western Kenya. Medical and Veterinary Entomology. 2007, 21: 103-111. 10.1111/j.1365-2915.2007.00672.x.
Burkett-Cadena ND, Graham SP, Hassan HK, Guyer C, Eubanks MD, Katholi CR, Unnasch TR: Blood Feeding Patterns of Potential Arbovirus Vectors of the Genus Culex Targeting Ectothermic Hosts. American Journal of Tropical Medicine and Hygiene. 2008, 79: 809-815.
Nasci RS, Edman JD: Blood-Feeding Patterns of Culiseta melanura (Diptera, Culicidae) and Associated Sylvan Mosquitoes in Southeastern Massachusetts Eastern Equine Encephalitis Enzootic Foci. Journal of Medical Entomology. 1981, 18: 493-500.
Apperson CS, Hassan HK, Harrison BA, Savage HM, Aspen SE, Farajollahi A, Crans W, Daniels TJ, Falco RC, Benedict M: Host feeding patterns of established and potential mosquito vectors of West Nile virus in the eastern United States. Vector-Borne and Zoonotic Diseases. 2004, 4: 71-82. 10.1089/153036604773083013.
Savage HM, Aggarwal D, Apperson CS, Katholi CR, Gordon E, Hassan HK, Anderson M, Charnetzky D, McMillen L, Unnasch EA, Unnasch TR: Host choice and West Nile virus infection rates in blood-fed mosquitoes, including members of the Culex pipiens complex, from Memphis and Shelby County, Tennessee, 2002-2003. Vector-Borne and Zoonotic Diseases. 2007, 7: 365-386. 10.1089/vbz.2006.0602.
Sota T, Mogi M: Effectiveness of Zooprophylaxis in Malaria Control - a Theoretical Inquiry, with a Model for Mosquito Populations with 2 Bloodmeal Hosts. Medical and Veterinary Entomology. 1989, 3: 337-345. 10.1111/j.1365-2915.1989.tb00240.x.
Saul A: Zooprophylaxis or zoopotentiation: the outcome of introducing animals on vector transmission is highly dependent on the mosquito mortality while searching. Malaria Journal. 2003, 2: 32-10.1186/1475-2875-2-32.
Renshaw M, Service MW, Birley MH: Density-Dependent Regulation of Aedes cantans (Diptera, Culicidae) in Natural and Artificial Populations. Ecological Entomology. 1993, 18: 223-233. 10.1111/j.1365-2311.1993.tb01094.x.
Kelly DW: Why are some people bitten more than others?. Trends in Parasitology. 2001, 17: 578-581. 10.1016/S1471-4922(01)02116-X.
Keesing F, Holt RD, Ostfeld RS: Effects of species diversity on disease risk. Ecology Letters. 2006, 9: 485-498. 10.1111/j.1461-0248.2006.00885.x.
Celli A: The history of malaria in the Roman Campagna from ancient times. 1933, London: J. Bale, sons & Danielsson, ltd
Celli A: La Malaria in Italia Durante il 1907. Ricerche Epidemiologiche e profilatiche. Atti della Società per gli studi della Malaria. 1908, 9: 675-729.
Fantini B: Anophelism without malaria: an ecological and epidemiological puzzle. Parassitologia. 1994, 36: 83-106.
Hackett LW, Missiroli A: The natural disappareance of malaria in certain regions of Europe. American Journal of Hygiene. 1931, 13: 57-78.
Molyneux DH: Common themes in changing vector-borne disease scenarios. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2003, 97: 129-132. 10.1016/S0035-9203(03)90097-6.
Nuttall GHF: Blood Immunity and Blood Relationships. Cambridge. 1904
Gozony L, Hindle E, Ross PH: Serological Tests. I. On the persistence of precipitins in sera stored in vitro. II. On the reactions obtained with (a) complement fixation test and (b) precipitin test, with the gut contents of blood-sucking arthropods. Journal of Hygiene. 1914, 14: 345-349. 10.1017/S0022172400005891.
Crans WJ: An agar gel diffusion method for the identification of mosquito blood-meals. Mosquito News. 1969, 29: 563-566.
Eliason DA: Identification of mosquito blood meals: A comparison of selected procedures. UNC. 1971
Burkot TR, Goodman WG, DeFoliart GR: Identification of mosquito blood meals by enzyme-linked immunosorbent assay. American Journal of Tropical Medicine and Hygiene. 1981, 30: 1336-1341.
Chow E, Wirtz RA, Scott TW: Identification of blood meals in Aedes aegypti by antibody sandwich enzyme-linked immunosorbent assay. J Am Mosq Control Assoc. 1993, 9: 196-205.
Bryan JH, Smalley ME: The use of ABO blood groups as markers for mosquito biting studies. Transactions of the Royal Society of Tropical Medicine and Hygiene. 1978, 72: 357-360. 10.1016/0035-9203(78)90127-X.
Coulson RM, Curtis CF, Ready PD, Hill N, Smith DF: Amplification and analysis of human DNA present in mosquito bloodmeals. Medical and Veterinary Entomology. 1990, 4: 357-366. 10.1111/j.1365-2915.1990.tb00452.x.
Darbro JM, Dhondt AA, Vermeylen FM, Harrington LC: Mycoplasma gallisepticum infection in house finches (Carpodacus mexicanus) affects mosquito blood feeding patterns. American Journal of Tropical Medicine and Hygiene. 2007, 77: 488-494.
Kent RJ: Molecular methods for arthropod bloodmeal identification and applications to ecological and vector-borne disease studies. Molecular Ecology Resources. 2009, 9: 4-18. 10.1111/j.1755-0998.2008.02469.x.
Triant DA, DeWoody JA: The occurrence, detection, and avoidance of mitochondrial DNA translocations in mammalian systematics and phylogeography. Journal of Mammology. 2008, 88: 908-922. 10.1644/06-MAMM-A-204R1.1.
Steuber S, Abdel-Rady A, Clausen PH: PCR-RFLP analysis: a promising technique for host species identification of blood meals from tsetse flies (Diptera: Glossinidae). Parasitology Research. 2005, 97: 247-254. 10.1007/s00436-005-1410-y.
Humair PF, Douet V, Cadenas FM, Schouls LM, Pol Van De I, Gern L: Molecular identification of bloodmeal source in Ixodes ricinus ticks using 12S rDNA as a genetic marker. Journal of Medical Entomology. 2007, 44: 869-880. 10.1603/0022-2585(2007)44[869:MIOBSI]2.0.CO;2.
This work was funded by a Gorgas Research Award from the American Society of Tropical Medicine and Hygiene (LFC), Emory University and NSF Ecology of Infectious Disease Grant 0840403. We also thank the NIH-RAPIDD program on Mosquito-borne diseases for promoting discussions leading to this manuscript.
The authors declare that they have no competing interests.
LFC proposed the research idea, designed research, wrote an initial ms draft, and wrote additional files 1, 2 &4. LCH wrote additional file 3. Database search and study selection was done by CLK and AMN, with feedback from LFC, LCH & UDK. CLK prepared Tables 1 and 2. AMN prepared additional files 5, 6 &7. UDK helped to write additional file 2, provided comments on the spatial scale of studies. All authors performed research, contributed to the final draft, and have read and approved the manuscript.
Electronic supplementary material
Additional file 2: Zooprophylaxis and dilution effect: the same natural history seen with different glasses [95, 99–104]. Support information. (PDF 70 KB)
Additional file 4: A primer of null model testing of vector blood-feeding patterns . Support information. (PDF 70 KB)
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Chaves, L.F., Harrington, L.C., Keogh, C.L. et al. Blood feeding patterns of mosquitoes: random or structured?. Front Zool 7, 3 (2010) doi:10.1186/1742-9994-7-3
- Host Species
- Mosquito Species
- Vertebrate Host
- Niche Shift
- Host Choice