Figure 14

Brain and muscle development of P. dumerilii , 40-46 hpf, apical view, dorsal side up. The age of the larvae in each row is given in the lower left corner of the first picture of each row. The displayed staining is indicated at the bottom of each column. A, F, K, P: The dorsal and ventral branch of the circumesophageal connectives (drcc and vrcc), the cerebral commissures (cc) and the dorsal unpaired axon (ua) are well visible, the prototroch ring nerve (ptrn) is well developed. B, G, L, Q: Four serotonergic cells are visible in the brain (the central one marked with a white star, the more dorsal ones marked with white arrows). G, D, H, I, M, N, R, S: The dorsal longitudinal muscles (dlm) and ventral longitudinal muscles (vlm) are intensely stained. E, J, O, T: The stomodeum (sto) becomes visible in the episphere from around 42 hpf onwards. CLSM microscopy, maximum projection, Imaris surpass mode. Scale bar in all images 20 μm. Further abbreviations see abbreviations list.