Table 2 P rimers used for qRT-PCR
Gene name | Abbreviation | Primer sequence | Amplicon size (bp) | Accession numbers |
|---|---|---|---|---|
Sodium–hydrogen exchanger 3 | NHE3 | F 5′- GGCTGTCTTCCAAGAAATGGGTGT -3′ | 168 | KJ451615 |
R 5′- AAGAACTTGGCAACACCAAGAGCG -3′ | ||||
Vacuolar-type H + −ATPase | VHA | F 5′- ACGTGAGGGCAGTGTCAGTATTGT -3′ | 161 | ADM67602.1 |
R 5′- TGATCAGCCAGTTGATGGAAGGGA -3′ | ||||
Na+, K + −ATPase | NKA | F 5′- CCGTGCTGAATTTAAGGCAGGTCA -3′ | 83 | GQ153672.1 |
R 5′- GCAAAGCTGATTCAGAAGCGTCAC -3′ | ||||
Rhesus protein | RhP | F 5′-GCACAAAGGAAAGCTGGACATGGT-3′ | 179 | KJ451616 |
R 5′-AATGATACCAGCCACCACTCCGA-3′ | ||||
Sodium-bicarbonate cotransporter | NBC | F 5′-AATTCGCTGCATGATTGTCCGTCC-3′ | 188 | HM157263.1 |
R 5′-TTCCGGAGAACTGACGACCGATTT-3′ | ||||
Cytosolic Carbonic anhydrase | CAc | F 5′-GTGAAGCCAACATGGAAGTC-3′ | 108 | KJ451614 |
R 5′-GCAGTTTGTAAGGAGTTGTCTC-3′ | ||||
Reference gene | ||||
Ubiquitin-conjugated enzyme | UBC | F 5′- ATGCAGATGGCAGTATTTGCCTGG -3′ | 127 | HM157280.1 |
R 5′- TTATTGGCTGGGCTGTTTGGGTTC -3′ |