Temperature tolerance of different larval stages of the spider crab Hyas araneus exposed to elevated seawater PCO2

Table 1 Results of two-way repeated measures ANOVAs

Stage	Response variable	CO ₂effect			Temperature effect			Interaction
		F	df	p	F	df	p	F	df	p
Zoea I	Oxygen consumption	0.723	1	0.411	32.838	6	< 0.001	1.709	6	0.133
Zoea II	Oxygen consumption	5.229	1	0.041	51.374	6	< 0.001	11.025	6	0.001
Megalopa	Oxygen consumption	0.965	1	0.345	7.595	6	< 0.001	2.769	6	0.022
Zoea I	Heart rate	0.000	1	0.978	32.255	6	< 0.001	0.712	6	0.642
Zoea II	Heart rate	0.001	1	0.974	41.980	6	< 0.001	18.755	6	< 0.001
Megalopa	Heart rate	0.136	1	0.723	28.959	6	< 0.001	0.419	6	0.862
Megalopa	Maxilliped beat rate	1.277	1	0.295	4.789	6	< 0.001	0.433	6	0.852
Zoea II	Maxilliped beat rate	1.109	1	0.333	18.092	6	< 0.001	1.289	6	0.288

ANOVAs were conducted to investigate effects of CO₂ and temperature on oxygen consumption (Figure 1A-C), heart rate (Figure 2A-C) and maxilliped beat rate (Figure 3A and B) of Hyas araneus zoea and megalopa larvae. Bold values indicate statistical significance.

ISSN: 1742-9994