Gene | Stage | Day | Heat shock effect | CO 2effect | Interaction | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|
 | F | df | p | F | df | p | F | df | p | ||
Cellular stress/heat shock response | |||||||||||
HSP70_1 | Zoea I | 15 | 165.2 | 1 | <0.001 | 3.3 | 1 | 0.088 | 1.8 | 1 | 0.198 |
 | Zoea II | 3 | 104.8 | 1 | <0.001 | 4.2 | 1 | 0.056 | 12.1 | 1 | 0.003 |
 | Zoea II | 15 | 127.2 | 1 | <0.001 | 7.4 | 1 | 0.017 | 8.8 | 1 | 0.011 |
 | Megalopa | 3 | 42.8 | 1 | <0.001 | 0.05 | 1 | 0.823 | 0.4 | 1 | 0.535 |
HSP70_2 | Zoea I | 15 | 114.3 | 1 | <0.001 | 3.2 | 1 | 0.091 | 0.02 | 1 | 0.870 |
 | Zoea II | 15 | 106.5 | 1 | <0.001 | 10.6 | 1 | 0.006 | 2.9 | 1 | 0.108 |
 | Megalopa | 3 | 136.8 | 1 | <0.001 | 1.5 | 1 | 0.232 | 2.5 | 1 | 0.129 |
HSP70_3 | Zoea I | 15 | 15.6 | 1 | 0.001 | 2.9 | 1 | 0.104 | 0.1 | 1 | 0.740 |
 | Zoea II | 3 | 11.4 | 1 | 0.004 | 0.8 | 1 | 0.369 | 0.009 | 1 | 0.922 |
 | Zoea II | 15 | 43.8 | 1 | <0.001 | 5.4 | 1 | 0.038 | 2.67 | 1 | 0.128 |
 | Megalopa | 3 | 23.2 | 1 | <0.001 | 1.8 | 1 | 0.192 | 0.09 | 1 | 0.758 |
HSP70_4 | Zoea I | 15 | 29.5 | 1 | <0.001 | 13.4 | 1 | 0.002 | 0.1 | 1 | 0.668 |
 | Zoea II | 3 | 45.0 | 1 | <0.001 | 8.7 | 1 | 0.009 | 5.4 | 1 | 0.333 |
 | Zoea II | 15 | 51.5 | 1 | <0.001 | 6.5 | 1 | 0.024 | 0.1 | 1 | 0.674 |
 | Megalopa | 3 | 30.0 | 1 | <0.001 | 0.05 | 1 | 0.813 | 4.6 | 1 | 0.046 |
HSP90 | Zoea I | 15 | 33.2 | 1 | <0.001 | 1.2 | 1 | 0.282 | 0.03 | 1 | 0.846 |
 | Zoea II | 3 | 7.7 | 1 | 0.015 | 0.7 | 1 | 0.388 | 3.4 | 1 | 0.085 |
 | Zoea II | 15 | 171.4 | 1 | <0.001 | 10.1 | 1 | 0.007 | 30.4 | 1 | <0.001 |
 | Megalopa | 3 | 79.8 | 1 | <0.001 | 2.6 | 1 | 0.123 | 6.4 | 1 | 0.022 |
HSP26 | Zoea I | 15 | 1.2 | 1 | 0.283 | 6.4 | 1 | 0.022 | 0.5 | 1 | 0.477 |
 | Zoea II | 15 | 15.2 | 1 | 0.002 | 1.3 | 1 | 0.267 | 0.2 | 1 | 0.660 |
 | Megalopa | 3 | 3.5 | 1 | 0.080 | 3.6 | 1 | 0.074 | 1.5 | 1 | 0.229 |
HSP60 | Zoea I | 15 | 0.6 | 1 | 0.430 | 1.9 | 1 | 0.182 | 0.5 | 1 | 0.473 |
 | Zoea II | 3 | 3.8 | 1 | 0.068 | 0.003 | 1 | 0. .955 | 0.1 | 1 | 0.662 |
 | Zoea II | 15 | 0.5 | 1 | 0.491 | 7.2 | 1 | 0.019 | 0.03 | 1 | 0.857 |
 | Megalopa | 3 | 1.4 | 1 | 0.246 | 2.6 | 1 | 0.126 | 0.3 | 1 | 0.537 |
Acid–base regulation | |||||||||||
CA | Zoea I | 15 | 0.1 | 1 | 0.746 | 1.3 | 1 | 0.257 | 0.1 | 1 | 0.670 |
 | Zoea II | 3 | 12.9 | 1 | 0.002 | 6.5 | 1 | 0.02 | 0.8 | 1 | 0.383 |
 | Zoea II | 15 | 6.1 | 1 | 0.028 | 22.8 | 1 | <0.001 | 11.2 | 1 | 0.005 |
 | Megalopa | 3 | 1.1 | 1 | 0.302 | 6.9 | 1 | 0.018 | 5.0 | 1 | 0.039 |
NaK | Zoea I | 15 | 1.1 | 1 | 0.304 | 1.7 | 1 | 0.209 | 0.2 | 1 | 0.628 |
 | Zoea II | 3 | 49.2 | 1 | <0.001 | 0.6 | 1 | 0.437 | 0.8 | 1 | 0.370 |
 | Zoea II | 15 | 0.2 | 1 | 0.662 | 5.9 | 1 | 0.031 | 1.3 | 1 | 0.272 |
 | Megalopa | 3 | 2.5 | 1 | 0.127 | 0.1 | 1 | 0.737 | 0.04 | 1 | 0.829 |
NBC | Zoea I | 15 | 0.3 | 1 | 0.579 | 12.8 | 1 | 0.003 | 0.7 | 1 | 0.393 |
 | Zoea II | 3 | 3.7 | 1 | 0.074 | 35.8 | 1 | <0.001 | 0.06 | 1 | 0.800 |
 | Zoea II | 15 | 6.2 | 1 | 0.027 | 10.3 | 1 | 0.007 | 5.8 | 1 | 0.031 |
 | Megalopa | 3 | 0.7 | 1 | 0.403 | 6.5 | 1 | 0.022 | 3.1 | 1 | 0.095 |
NKCC | Zoea I | 15 | 0.5 | 1 | 0.471 | 5.6 | 1 | 0.030 | 0.1 | 1 | 0.670 |
 | Zoea II | 3 | 0.4 | 1 | 0.490 | 0.003 | 1 | 0.955 | 0.1 | 1 | 0.666 |
 | Zoea II | 15 | 0.002 | 1 | 0.963 | 2.9 | 1 | 0.109 | 0.03 | 1 | 0.862 |
 | Megalopa | 3 | 18.3 | 1 | <0.001 | 1.0 | 1 | 0.333 | 9.7 | 1 | 0.007 |
Mitochondrial energy metabolism | |||||||||||
PDH | Zoea I | 15 | 3.2 | 1 | 0.093 | 0.3 | 1 | 0.578 | 0.3 | 1 | 0.582 |
 | Zoea II | 3 | 9.0 | 1 | 0.009 | 2.5 | 1 | 0.134 | 3.0 | 1 | 0.101 |
 | Zoea II | 15 | 0.07 | 1 | 0.794 | 0.1 | 1 | 0.69 | 0.3 | 1 | 0.577 |
 | Megalopa | 3 | 1.1 | 1 | 0.297 | 14.3 | 1 | 0.002 | 0.6 | 1 | 0.438 |
IDH | Zoea II | 3 | 4.9 | 1 | 0.045 | 2.4 | 1 | 0.144 | 0.03 | 1 | 0.862 |
 | Zoea II | 15 | 1.2 | 1 | 0.285 | 13.8 | 1 | 0.002 | 6.9 | 1 | 0.019 |
 | Megalopa | 3 | 0.01 | 1 | 0.915 | 5.7 | 1 | 0.029 | 2.0 | 1 | 0.170 |
NAD | Zoea I | 15 | 1.4 | 1 | 0.247 | 6.0 | 1 | 0.025 | 0.8 | 1 | 0.379 |
 | Zoea II | 3 | 0.7 | 1 | 0.388 | 9.1 | 1 | 0.008 | 0.003 | 1 | 0.953 |
 | Zoea II | 15 | 6.8 | 1 | 0.021 | 42.9 | 1 | <0.001 | 11.2 | 1 | 0.005 |
 | Megalopa | 3 | 0.01 | 1 | 0.919 | 6.3 | 1 | 0.023 | 0.9 | 1 | 0.348 |
SDH | Zoea I | 15 | 0.005 | 1 | 0.943 | 6.6 | 1 | 0.020 | 0.7 | 1 | 0.388 |
 | Zoea II | 3 | 14.5 | 1 | 0.002 | 10.7 | 1 | 0.005 | 0.03 | 1 | 0.848 |
 | Zoea II | 15 | 0.3 | 1 | 0.565 | 1.6 | 1 | 0.221 | 1.6 | 1 | 0.221 |
 | Megalopa | 3 | 0.1 | 1 | 0.704 | 0.02 | 1 | 0.881 | 1.2 | 1 | 0.276 |
CCR | Zoea I | 15 | 0.2 | 1 | 0.604 | 2.9 | 1 | 0.105 | 2.2 | 1 | 0.155 |
 | Zoea II | 3 | 1.7 | 1 | 0.210 | 7.2 | 1 | 0.016 | 11.2 | 1 | 0.004 |
 | Zoea II | 15 | 8.6 | 1 | 0.011 | 0.7 | 1 | 0.405 | 1.0 | 1 | 0.314 |
 | Megalopa | 3 | 0.1 | 1 | 0.721 | 6.1 | 1 | 0.025 | 3.4 | 1 | 0.082 |
COX | Zoea I | 15 | 16.7 | 1 | <0.001 | 6.1 | 1 | 0.025 | 0.3 | 1 | 0.589 |
 | Zoea II | 3 | 32.2 | 1 | <0.001 | 2.0 | 1 | 0.168 | 13.7 | 1 | 0.002 |
 | Zoea II | 15 | 4.2 | 1 | 0.060 | 11.6 | 1 | 0.005 | 13.6 | 1 | 0.003 |
 | Megalopa | 3 | 15.9 | 1 | 0.001 | 0.4 | 1 | 0.491 | 6.4 | 1 | 0.022 |
atpA | Zoea II | 3 | 5.0 | 1 | 0.041 | 11.9 | 1 | 0.003 | 12.5 | 1 | 0.003 |
 | Zoea II | 15 | 3.4 | 1 | 0.088 | 0.2 | 1 | 0.661 | 2.1 | 1 | 0.169 |
 | Megalopa | 3 | 0.1 | 1 | 0.710 | 0.7 | 1 | 0.387 | 0.5 | 1 | 0.454 |