Figure 5

Gene expression of pre- and post-apolysis Calanus finmarchicus C5 copepodids measured via qPCR 8 and 10 days after the penultimate molt. Each of the subpanels (a-f) indicates the expression level of an individual gene, which is specified on the y-axis. Data consist of n = 3 samples for the pre-apolysis group and n = 4 samples for the post-apolysis group (each sample consisted of 3 homogenized copepods of known molt stage). Expression was normalized by 4 housekeeping genes and log₂ transformed. Negative numbers indicate lower expression than the geometric mean expression of the 4 housekeeping genes (see Methods for additional detail). Results shown for only those genes with significantly different expression between pre- and post-apolysis (Student’s t-test, p < 0.05).