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Fig. 5 | Frontiers in Zoology

Fig. 5

From: Positional RNA-Seq identifies candidate genes for phenotypic engineering of sexual traits

Fig. 5

RNAi phenotype and ISH pattern of the [+,0,0] class candidates RNA815_7008 and RNA815_9973.1. a Control ISH pattern of RNA815_7008 in the testis region. (b) Detail of control ISH pattern of RNA815_7008 in the testis. (c) Control sperm. (d) RNA815_7008 knock-down sperm. (e) RNA815_7008 knock-down testis. (f) Control testis. (g) Control vas deferens in the ovary region. (h) RNA815_9973.1 knock-down vas deferens in the ovary region. (i) RNA815_9973.1 knock-down testis. (j) Control ISH pattern of RNA815_9973.1 in the testis region. (k) Detail of control ISH pattern of RNA815_9973.1 in the testis. Black and white dotted lines delimit the testis and the testis centre respectively. Note the level of the testes (black triangles) and the ovaries (white triangles). Testis periphery (tp); testis centre (tc); sperm anterior feeler (af), body (b), bristles (br), shaft (s) and terminal brush (tb); vas deferens (arrows). Bars = 50 μm (A, C, D, E, H, I, J), 30 μm (B, K), 20 μm (F, G)

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