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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Neural architecture of Galathowenia oculata Zach, 1923 (Oweniidae, Annelida)

Fig. 3

Tubulin-like immunoreactive (−lir) nervous system architecture in the head region and ultrastructure of the dorsal commissure of Galathowenia oculata. Labeling with antibodies against acetylated α-tubulin (green) and serotonin (red; shown only in g); nuclei labeling with DAPI (blue). Maximum projections and whole-amount specimens (ac, eh). TEM micrograph (d). a Color-coded image of the tubulin-lir dorsal commissure, ventral nerve cord and two pairs of the main peripheral longitudinal neurite bundles, lateral view, ventral side is to the left. b, c Lateral views show the nervous system within the head, note the collateral neurite bundles along the ventral nerve cord (arrows), ventral side is to the left. d Intraepidermal neurite bundles of the dorsal commissure (shown by bracket), transverse section of the head collar. e Tubulin-lir ventral nerve cord with collateral neurite bundles (arrows), trunk region; dorsal view, anterior is to the top. f Tubulin-lir ventral nerve cord in the posterior-most segments and pygidium, note cilia covering the surface, ventral side is to the top. g, h Tubulin-lir and serotonin-lir (in G) and only tubulin-lir (in H) ventral nerve cord with numerous serially arranged circular neurite bundles: transverse section of trunk region, note the paired structure of the nerve cord, ventral side is to the bottom. cc - circumesophageal connectives, ccc - neurites interconnecting cc, cib – ciliary band, cil – cilia, dc - dorsal commissure, dln – main dorsolateral neurite bundles, dn – main dorsal neurite bundle, e – eye, ecm – exctracellular matrix, ep – epidermis, exn – epidermal plexus within the outer side of hc, hc – head collar, mc – muscle cells, py - pygidium, tcn – tubulin-lir serially arranged circular neurite bundles, vlb – main ventrolateral neurite bundle, vnc – ventral nerve cord, vpo – ventral pharyngeal organ

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