Fig. 7
Representative 2-OH-E+ fluorescence patterns (O2 •- formation) in M. edulis gills. a: Overview of gill filaments, a1: blue-range fluorescence image (400–440 nm), a2: red-range fluorescence image (620–660 nm), a3: corresponding transmission image. b: fluorescence intensity profile across a filament. Black box indicates limits of blood sinus. Outer and inner region of epithelial cells were defined for statistical purposes as described in the method section. c: View of the ventral bend with subepithelial mucus glands containing mucus vacuoles (white arrows). c1: blue-range fluorescence image (400–440 nm), c2: red-range fluorescence image (620–660 nm), c3: corresponding transmission image. Bs = blood sinus, ore = outer region of epithelial cells, ire = inner region of epithelial cells, mc = mucocyte, ROI = region of interest. Scale bar = 25 μm