Table 1 Analysis conditions for each of the dyes used during the study

Ageladine-A (in DMSO)

Probe which exists as a nearly uncharged monomer under pH of 8.1–8.6. After crossing cellular membranes, becomes charged in the cytosol and acidic compartments of cells.

3

30

90

MP 760

-

420-500

-

Average intensity

C-H₂DFFDA (in Ethanol)

Non-fluorescent molecule which is converted to a green-fluorescent form (DCF) when the acetate groups are removed by intracellular esterases and oxidation occurs in the cell.

3

20

30

488

-

510-550

-

Average Intensity

DAF-2DA (in DMSO)

DAF-2 is formed by intracellular hydrolization of its ester bonds by esterases. It remains essentially non-fluorescent until it reacts with nitrosonium cation (forming the fluorescent DAF-2 T) and such fluorescence increases in a NO-dependent manner.

5

20

30

488

-

505-525

-

Average Intensity

Dihydroethidium (DHE) (in DMSO)

Regularly shows a blue emission when excited with a 355 nm laser. When oxidized to 2-dihydroxiethidium (2-OH-E⁺) by the presence of O₂ ⁻, it intercalates with the DNA and shows a red emission when excited with an argon laser.

5

10

30

MP 710

488

400–440

620–660

Ratio PMT2/PMT1

JC-1 and JC-10 (in DMSO)

Green fluorescent probe which exists as a monomer at low Δ_ψm. With high Δ_ψm values, JC-1 and JC-10 aggregates and shows a red fluorescence.

3

10

60

488

488

500–550

560–600

Ratio PMT1/PMT2

Lysotracker Red DND-99 (in DMSO)

Cell permeable fluorophore, which through protonation, concentrates on the membranes of spherical acidic organelles.

3

0.07

5

577

-

580–620

-

Average intensity

MitoTracker Deep Red 633 (in DMSO)

Molecule which becomes evidently fluorescent once accumulates in the lipid environment of the mitochondria.

4

1

60

633

-

640–680

-

Average intensity

Syto-13 (in DMSO)

Dye which upon binding to nucleic acid exhibits a green fluorescence.

3

10

60

488

-

500–520

-

Average Intensity