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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Visualization of the 3D structures of small organisms via LED-SIM

Fig. 3

Comparison of the 3D images of a freshly hatched water flea Daphnia sp. obtained by CLSM (Leica TCS SP8) and LED-SIM. a CLSM image, a maximum-intensity projection of 150 frames along the Z-axis over a 147.2 μm thickness under a 25× objective lens (Additional file 1: Movie S1); b A single section obtained by CLSM; c A rectangular area digitally zoomed from inset (b). d LED-SIM image of the same sample, a maximum-intensity projection of 113 frames along the Z-axis under a 20× objective lens (Additional file 2: Movie S2); e A single section of obtained LED-SIM image; f A rectangular area digitally zoomed from inset (e)

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