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Fig. 4 | Frontiers in Zoology

Fig. 4

From: Visualization of the 3D structures of small organisms via LED-SIM

Fig. 4

Comparison of LED-SIM image with Micro-CT and conventional optical microscope images. a Conventional microscopy image of spermatheca of feather-winged beetle Acrotrichis sp., stacks of images taken by a Zeiss Axiostar plus microscope and composed by Helicon Focus 6 software (Helicon Soft Ltd., Kharkov, Ukraine). b Maximum-intensity projection of a meta-femur of Clavicornaltica sp., obtained by LED-SIM with 3 subviews, 67 frames along the Z-axis under a 20× objective lens, obtained in 4 min, showing the spring tendon and muscle bundles inside the meta-femur. c, d Single sections of an obtained LED-SIM image. e LED-SIM image of spermatheca of Acrotrichis sp. (Additional file 3: Movie S3), generated by Amira 5.4.1 software. f Meta-femur of flea beetle Clavicornaltica sp., a maximum-intensity projection image of Micro-CT (MicroXCT-400, Carl Zeiss X-ray Microscopy, Inc., Pleasanton, USA) with 1000 frames along the Z-axis under a 40× objective lens, consumed approximately 24 h on scanning. g, h Single sections of obtained Micro-CT image

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