- Open Access
Muscle development in the shark Scyliorhinus canicula: implications for the evolution of the gnathostome head and paired appendage musculature
Frontiers in Zoology volume 14, Article number: 31 (2017)
The origin of jawed vertebrates was marked by profound reconfigurations of the skeleton and muscles of the head and by the acquisition of two sets of paired appendages. Extant cartilaginous fish retained numerous plesiomorphic characters of jawed vertebrates, which include several aspects of their musculature. Therefore, myogenic studies on sharks are essential in yielding clues on the developmental processes involved in the origin of the muscular anatomy.
Here we provide a detailed description of the development of specific muscular units integrating the cephalic and appendicular musculature of the shark model, Scyliorhinus canicula. In addition, we analyze the muscle development across gnathostomes by comparing the developmental onset of muscle groups in distinct taxa. Our data reveal that appendicular myogenesis occurs earlier in the pectoral than in the pelvic appendages. Additionally, the pectoral musculature includes muscles that have their primordial developmental origin in the head. This culminates in a tight muscular connection between the pectoral girdle and the cranium, which founds no parallel in the pelvic fins. Moreover, we identified a lateral to ventral pattern of formation of the cephalic muscles, that has been equally documented in osteichthyans but, in contrast with these gnathostomes, the hyoid muscles develop earlier than mandibular muscle in S. canicula.
Our analyses reveal considerable differences in the formation of the pectoral and pelvic musculatures in S. canicula, reinforcing the idea that head tissues have contributed to the formation of the pectoral appendages in the common ancestor of extant gnathostomes. In addition, temporal differences in the formation of some cranial muscles between chondrichthyans and osteichthyans might support the hypothesis that the similarity between the musculature of the mandibular arch and of the other pharyngeal arches represents a derived feature of jawed vertebrates.
The origin of jawed vertebrates (gnathostomes) is undoubtedly one of the major events in the history of life, as it drastically changed the feeding modes among vertebrates . The origin of jaws, paired fins, and the cephalic and appendicular musculature was probably of chief importance for the transition from suspension feeding to predation in this particular vertebrate lineage . These novel morphological features may also have contributed to the vast radiation of gnathostomes, which make up more than 99.9% of all living vertebrates . Chondrichthyans such as the sharks are considered to have morphological characteristics that retained various plesiomorphic gnathostome traits [3, 4]. These cartilaginous fishes have been around for over 400 million years and are, therefore, amongst the oldest surviving vertebrate groups . Although they have unique features that evolved in ways distinct from other fishes, they possess, for example, the most plesiomorphic paired fin structure of modern vertebrates . Therefore, sharks are also relevant for discussions on the development and evolution of not only fish muscles but also of the muscles of vertebrates as a whole . Moreover, striking similarities were detected between the musculature of chondrichthyans and placoderms, which are fossil representatives of the most basal gnathostomes [7, 8]. This makes them ideal extant models to study the evolution of paired (i.e., pectoral and pelvic) appendage musculature, providing a unique opportunity to investigate the developmental processes involved in the formation of these tissues during early evolution of gnathostomes [9,10,11]. Sharks, in addition, belong to the living sister group of osteichthyans (bony fish + tetrapods), where developmental studies are mostly performed, which makes them fundamental models for phylogenetic sampling (e.g., [10,11,12]). Data from cyclostomes - which are the only extant representatives of agnathans - such as the lamprey are also extremely relevant to understand the origin and early evolution of gnathostome morphology [13,14,15,16,17,18,19,20].
Comparative analyses have provided descriptions of the musculature of the head, neck, and locomotory appendages across various vertebrate lineages [18, 21,22,23,24,25,26]. However, little information is available on how and when each of the specific muscles develop in organisms that may have retained plesiomorphic gnathostome features, as with the shark. Currie and colleagues used shark models to investigate how the mechanisms that generate appendicular muscles evolved [12, 27]. These authors confirmed the observations obtained in studies carried out at the end of the nineteenth century showing that, in sharks, the appendicular musculature is formed by epithelial somitic extensions that penetrate the fin buds during development. However, their work was not focused on providing a detailed description on the development of specific muscles, such as those that connect the pectoral appendages to the skull. The only studies providing details on the development of individual muscles of sharks were published several decades ago (e.g., cephalic musculature reviewed by Edgeworth ), but they lack validation with novel methodological approaches. Therefore, detailed studies on shark muscle development are required to further explore potential ancient developmental processes involved in the formation of the cephalic and appendicular musculature in gnathostomes.
Several studies suggest a striking conservation of the developmental patterning of cephalic muscles, which is particularly well documented in amphibians [28,29,30,31]. These include the observation that these muscles tend to differentiate from the anterior to the posterior. For example, mandibular and hyoid muscles normally appear earlier than the muscles of the branchial (i.e., the most posterior pharyngeal) arches. Their development also tends to follow a lateral to medial direction. For instance, lateral muscles of one arch tend to differentiate earlier than the more medially and ventral muscles of the same arch. In addition, these muscles normally develop from their region of origin towards their region of insertion [28, 32, 33]. It remains unknown, however, if this temporal and spatial sequence of developmental events represents the plesiomorphic feature, present in the common ancestor of all gnathostomes. Diogo and colleagues suggested that, in general, the developmental order of appearance of the cephalic muscles of amphibians [28, 29] and zebrafish  parallels the evolutionary order of appearance. This is also the case for the cephalic muscles in the head, neck, and limb muscles of primates . Developmental studies on sharks are crucial to investigate if such patterns are also seen in chondrichthyans, exploring the conservation of these developmental patterns within gnathostomes, and whether there is a parallelism between ontogeny and phylogeny in vertebrate muscle development in general.
Within the broader analysis of cephalic muscle development in vertebrates, special attention has been given to the puzzling muscle cucullaris, which is deeply related to one of the most crucial evolutionary events during vertebrate evolutionary history: the evolution of the neck . Recently, Ziermann and colleagues proposed that neck evolution was a long, stepwise macroevolutionary event, involving a stage in which an undivided cucullaris was connected to the branchial arches and the pectoral girdles , followed by its subdivision into the levatores arcuum branchialium attaching to the branchial arches and the protractor pectoralis attaching to the pectoral girdle, in osteichthyans . Subsequently, there was further differentiation of the protractor pectoralis into various muscles (e.g., trapezius, sternocleidomastoideus) that took place during the evolution of tetrapods, where the head became further separated from the trunk . Remarkably, recent analyses of the musculature in placoderm fossils suggest that the cucullaris might not have attached to the pectoral girdle in at least some of the members of this extinct group . The comparison of the osteichthyans developmental data with the information obtained via the detailed analyses of the development of the cucullaris in chondrichthyans may offer additional information to discuss the ancestral condition of the cucullaris and thus the evolution of the neck within gnathostomes.
Additionally, comprehensive myological analyses in shark embryos may also help elucidate how paired appendage musculature was acquired within the gnathostome lineage. Two influential hypotheses were proposed during the late nineteenth century to explain the origin of two sets of paired appendages (pectoral and pelvic) in vertebrates: the gill - arch theory and the lateral fin-fold theory. The gill-arch theory proposes that pectoral and pelvic appendages evolved from modified gill arches and the pelvic appendages secondarily migrated caudally , whereas the lateral fin-fold theory suggests that pectoral and pelvic fins derive from a hypothetical bilateral continuous embryonic finfold. Both theories are consistent with the hypothesis that pectoral and pelvic appendages are serial homologous [38,39,40]. However, within these two theories the fin-fold theory, which lacks paleontological evidences [41, 42], excludes the contribution of head tissues to the formation of pectoral appendages. When molecular analyzes became available, the involvement of a common set of molecular mechanisms activated within a continuous dorsal/ventral field of competence to form appendages (“competent stripe”, ) during the development of not only paired but also unpaired appendages were consistent with this idea that all these appendages do share similar developmental mechanisms [9,10,11, 43,44,45,46]. However, Gillis and colleagues, have shown that there are remarkable similarities in the developmental mechanisms operating during the ontogeny of the branchial arches and pectoral fin development [47, 48], thus reigniting discussions on Gegenbaur’s hypothesis.
It is worth noting that the theories regarding the origin of two sets of paired appendages in gnathostomes mainly target the initial developmental components of fins, which are the fin mesenchyme and the endoskeleton that differentiates within and from it. However, dismissed from these theories are additional components essential for fin/limb function and which probably reinforced their adaptive rate, such as muscles, nerves, or blood vessels. Comparative myogenic studies performed by Diogo and colleagues, integrated with data from other authors and fields, suggest that the musculature of pectoral (fore-) and pelvic (hind-) appendages are particularly different in the proximal (girdle) region of these appendages [22, 49, 50]. These data question the existence of a common serial homologue musculature in pectoral and pelvic appendages, and indicate that what makes the pectoral and pelvic appendages so unique, and so remarkably similar in derived gnathostomes such as tetrapods, might in fact be the result of derived co-option . To gain insight into these questions, it is crucial to comparatively evaluate muscle development in the pectoral and pelvic appendages in animals retaining a plesiomorphic fin musculature within gnathostomes, the sharks, and evaluate the contribution of cranial muscles to appendicular muscles during their formation.
Therefore, to discuss the broader developmental and evolutionary issues mentioned above, we present a detailed timeline of the development of both the cephalic and paired appendicular muscles in a shark species, Scyliorhinus canicula. We identified heterochronic events during the development of the cephalic muscles of the shark as compared to the developmental pattern reported for most osteichthyans. That is, in our analyses of the shark the hyoid muscles develop earlier than mandibular muscles. This pattern contrasts with the observations in most osteichthyans where usually the mandibular muscles develop before the hyoid muscles or both groups develop simultaneously. In addition, we found that, although the development of the pectoral and pelvic appendicular muscles share similarities, there are significant differences concerning the timing of their formation. Moreover, tight muscular connections, involving several muscular units, develop between the pectoral girdle and the cranium of sharks, which finds no parallel during myogenesis of the pelvic fins. Our results highlight the importance to trace the distinct evolutionary processes analyzing different tissues individually and making use of model organisms at key phylogenetic positions.
Collection and staging of embryos
Scyliorhinus canicula (L. 1758) eggs were collected from the Menai Strait (North Wales). Embryos were isolated from egg cases and dissected from the yolk sac in ice-cold phosphate-buffered saline (PBS). Specimens were then staged according to Ballard et al. , before being fixed and processed as described below.
SEM and histology
For the scanning electron microscopy (SEM), specimens were fixed in 1% glutaraldehyde, then treated with 1% osmium tetroxide, dehydrated in a graded ethanol series, and transferred to acetone. Subsequently the specimens were critical-point dried, mounted onto carbon discs, sputter-coated with gold particles and visualized in a Jeol JSM-T300 Scanning Electron Microscope. For histology, embryos were fixed in 4% paraformaldehyde, dehydrated in a graded ethanol series, washed in Xylene, and embedded in paraffin. The resultant microtome sections (10 μm) were stained using Mallory’s Triple Stain.
S. canicula muscle development was characterized in the embryonic time comprising stages 23 to 32 using immunochemistry with antibody against Myosin Heavy Chain (MyHC; A4–1025, DSHB), a marker of muscle differentiation , following previously established and described protocols . We analyzed one embryo per stage for muscle development using immunochemistry, as our previous muscle developmental studies have indicated that the intraspecific variability concerning the timing of muscle development depends also on sampling density and here the stages were clearly separated .
Regarding immunochemistry, the specimens were fixed in 4% PFA, then washed in PBS with 1% triton (PBT-1) for 3 h, incubated in 0.25% trypsin for 2–5 min, rinsed in PBT-1, and immersed in pre-cooled acetone for 10 min. After a brief rinse in PBT-1, the embryos were placed in blocking solution containing 10% goat serum (GS), 1% dimethyl sulfoxide, and 5% H2O2 in PBT-1, overnight. The MyHC antibody was used in a concentration of 1:10 and was diluted in PBT-1 containing 10% GS. Goat anti-mouse IgG secondary antibody, HRP (Thermofisher), was used at a concentration of 1:500 diluted in PBT-1 with 1% GS. Embryos were then washed in 1% GS in PBT-1, followed by PBS, and then incubated in 0.5 mg/ml diaminobenzidine (DAB). The reaction was developed by transferring embryos to fresh DAB activated with 0.003% H2O2.
One side of the embryos was dissected with micro-dissection tools under a dissection microscope to analyze the development of deep muscles. The specimens were photographed at a dissecting microscope (Nikon SMZ-2B) equipped with a Nikon DS Fi1 5 Megapixel Color Camera Head. Myological terminology used in the present paper follows that proposed by Diogo and Abdala  and updated by Ziermann et al.  and Diogo and Ziermann  for adult sharks.
Pectoral fin development and muscle differentiation
The development of the cephalic and appendicular musculature of S. canicula is summarized in Table 1 and shown in Figs. 1, 2, 3, 4, 5 and 6. Muscle projections are detected extending from the myotome towards the pectoral fin fields between stages 26 and 27 (Fig. 1d-e; j-k). MyHC staining indicates that muscle projections invade the pectoral fin territory between stages 28 and 29 (Fig. 1l-m). The former shows the development of the dorsal muscle adductor superficialis and the ventral muscle abductor superficialis (Figs. 1l-m and 3c-d). At stage 30, muscle projections are detected throughout the fin, both dorsally and ventrally (Fig. 1f). MyHC staining further indicates that the abductor superficialis, which connects the girdle to the fin, is now prominent at this stage (Fig. 1n). MyHC staining also highlights the rostroventral development of the pterygialis cranialis at stage 28 (Fig. 4c). Between stages 31 and 32, the adductor superficialis and abductor superficialis pursue development (Figs. 1g-h, o-p; 6d), and the pterygialis cranialis continues to expand and differentiate without major changes relative to the previous stages (Figs. 5d, e and 6c). Finally, at stage 34 and prior to hatching, the appendicular skeleton appears strongly associated to the musculature both in the girdle and in the pectoral fins (Fig. 1i).
The first signs of pectoral fin development are detectable at stage 19, budding out from the lateral plate mesoderm and positioned approximately between somites 6 to 16 (Fig. 1a). By stage 23, the pectoral fin buds are visible lateral to the yolk stalk  (Fig. 1b). However, the apical ectodermal ridge (AER), a crucial signaling center during fin/limb development, is undetectable up to stage 24 (Fig. 1c). Then, the pectoral fin buds re-shape dorsoventrally acquiring a disc-like structure capped distally by the AER (Fig. 1c). This distal structure is rapidly converted into an apical ectodermal fold  (AEF) by stage 25 (Fig. 1d).
Pelvic fin development and muscle differentiation
The pelvic fins start to bud out from the lateral plate mesoderm laterally to the cloaca region at stage 25 and their development is close to completion prior to hatching  (Fig. 2a-f). As for the pectoral appendages, the formation of the AER seems to be transient, rapidly giving rise to an AEF, at stage 26 (Fig. 2b). As seen in the pectoral appendages, MyHC staining and histology suggest that muscle projections extend ventrally towards the base of the pelvic fins between stage 27 and stage 28 (Fig. 2c, g). At stage 30 an undivided abductor and the adductor superficialis can be identified (Fig. 2d, h). However, pelvic fins show an even fainter staining on both the dorsal and ventral sides indicating just the initial development of the adductor superficialis and abductor, respectively (compare Fig. 1n with Fig. 2h). The latter is easier to see at stage 31 (Fig. 2e, i). These muscles then expand along the proximodistal axis of the pelvic fins during stage 31 (Fig. 2e) reaching the margin of the terminal finfold by stage 32 (Fig. 6d). While formation of the endoskeletal elements continues at stage 33, the distinction between a proximal abductor and a distal abductor, as well as the presence of a separate muscle protractor of the pelvic fin as described in adult sharks , cannot be made. As these are superficial structures, it is likely that they are not differentiated yet in any of the stages analyzed by us, which is plausible due to the fact that the pelvic fins start to form later than the pectoral fins.
Cranial development and muscle differentiation
The first signs of eye and nasal pit development are detected in S. canicula at stage 17 and 21, respectively (data not shown). As early as stage 19, features such as the otic vesicles and the curvatures delineating the forebrain, the midbrain, and the hindbrain are visible . At stage 23, the ganglia of cranial nerves V and VII are clearly observable through the skin (Fig. 3a). Furthermore, six pharyngeal arches are present as anlagen and the proximity to the posteroventrally looping heart is clearly visible  (Fig. 3a). The first pharyngeal arch is the mandibular arch, the second is the hyoid arch, and the following four are branchial arches I-IV (i.e., pharyngeal arches III-VI). The first three pharyngeal pouches are open, with the first one being the spiracle (C1, hyomandibular or spiracular cleft). Only the myotomes and the cardiac wall show MyHC staining at this stage. The most anterior myotome has only its ventral half stained and is superior to the posterior half of the fourth pharyngeal arch.
By stage 24 the posterior branchial arches are better defined compared to the previous stage and the fourth pharyngeal cleft is visible. The hyoid arch and branchial arches I and II show the first external gill buds that reach from their arch posteriorly over the adjacent cleft. The gill buds decrease in size from anterior to posterior (Fig. 3b). Dorsally between branchial arches I and II is a small anteroposteriorly orientated muscle, which is the ventral portion of an anterior developing myotome (Fig. 3b). Anteriorly on the hyoid arch and branchial arches I-III, faint MyHC staining can be detected indicating the initial differentiation of the dorsal constrictor muscles (constrictor hyoideus of the hyoid arch, and constrictores branchiales I to III of the branchial arches) (Fig. 3b). The staining decreases from anterior to posterior, which is undetectable in the mandibular arch. By stage 26, the fifth pharyngeal cleft is visible and the first myotome, which lies dorsally between branchial arches III and IV, is larger and more visible than in previous stages (Fig. 3c). The external gill branches on the hyoid arch and branchial arches I and II expand and the first gills appear on branchial arch III.
In stage 27 the ventral portion of the most anterior differentiating myotome becomes visible dorsally between branchial arches I and II (Fig. 3d). The mandibular arch also shows an elongated thin muscle anlage anteroventrally located; this is the intermandibularis anlage, which is only separated by a small gap from the developing interhyoideus anlage of the hyoid arch (Fig. 3e). This interhyoideus anlage is continuous with the ventral portion of the constrictor hyoideus. The muscle anlagen of the dorsal constrictors extend and are more clearly visible (Fig. 3d, e): the hyoid arch includes the constrictor hyoideus anlage, while the branchial arches include the constrictores branchiales I-III (constrictores branchiales superficialis). These muscles are mainly dorsoventrally orientated, the constrictor hyoideus being the longest and stretching almost the entire length of the hyoid arch, and the constrictor branchialis III being the shortest, covering only half of the dorsoventral extension of the third branchial arch (Fig. 3d). The staining of the constrictors decreases from anterior (hyoid arch) to posterior (Fig. 3d) indicating the later development of more posterior muscles.
Major differences are then observed at stage 28 (Fig. 4a-c). External gills appear on the mandibular arch and all posterior external gills are lengthened. Dorsal extraocular muscles are visible, the mandibular arch muscle anlage is expanded, and, from a lateral view, the constrictor dorsalis of this arch is visible just rostral to the spiracle (Fig. 4a). The most rostral portion of the mandibular muscles is the developing preorbitalis, caudally adjacent to the anlage of the adductor mandibulae A2; and caudomedially there is the intermandibularis anlage (Fig. 4b). At this stage, all mandibular arch muscles derive originally from a single elongated anlage that then separates during later stages into several regions (dorsal, middle and ventral), which then gives rise to one or more muscles. The hyoid arch muscle anlage also differentiates into three portions (Fig. 4a, b): the most rostro-dorsal one is the anlage of the adductor arcus palatini, the large lateral one is the anlage of the constrictor hyoideus dorsalis, and the most ventral portion is the interhyoideus anlage. The adductor arcus palatini develops from the most rostrodorsal portion of the constrictor hyoideus primordium; this rostrodorsal portion also gives rise to the constrictor hyoideus dorsalis. The constrictores branchiales I-IV stretch almost the entire dorsoventral length of their respective arches (Fig. 4a). The ventral branchial muscles coracobranchiales and the hypobranchial muscles coracomandibularis and coracoarcualis are distinguishable from all the other cephalic muscles because they develop from their region of insertion (i.e., from the mandible and branchial arches, respectively; compare Figs. 4b, f, 5b, d for coracomandibularis and Figs. 4f, 5b, d, 6c for coracoarcualis), while the mandibular, hyoid and branchial muscles develop from their region of origin, as do most cephalic muscles of other vertebrates (Fig. 4b; compare for examples Figs. 3e, 4f, 5d, 6c for the development of the intermandibularis and interhyoideus).
At stage 29 the ventral extraocular muscles become visible (Fig. 4d). The differentiation of the mandibular muscles is now also noticeable from a lateral view (Fig. 4d). The dorsal constrictor separates into a dorsal part that extends posteriorly, the spiracularis, and a lateral portion, extending inferiorly and medially, the levator arcus palatini (Fig. 4d). The preorbitalis, adductor mandibulae A2 and intermandibularis can be distinguished (Fig. 4d, f). The hyoid muscles adductor arcus palatini and constrictor hyoideus dorsalis start to separate, while ventrally the interhyoideus muscle grows (Fig. 4d, f). Within the branchial muscles only four constrictores branchiales – extending from lateral to ventral regions – are clearly visible (Fig. 4e, f). Ventrally the coracobranchiales I-II appear as faint stains at the base of the branchial arches I-II (Fig. 4f). Dorsal to branchial arches I and II, there is a faint anteroposteriorly orientated muscle staining, which indicates the initial differentiation of the cucullaris (Fig. 4e). The hypobranchial muscles coracomandibularis and coracoarcualis are now more distinguishable than in stage 28, and the hypobranchial muscle sternohyoideus is faintly visible just rostral to the coracoarcualis (Fig. 4f).
In stage 30 the muscles described in stage 29 become more clearly separated (Fig. 5a), especially in ventral view (Fig. 5b). The main contrast to the former stage is that the cucullaris is now plainly visible as a thin band-like structure extending dorsally from branchial arch I to branchial arch IV (Fig. 5a). Ventrally the sternohyoideus becomes further distinguishable from the coracoarcualis (Fig. 5b). The coracobranchiales I-III can be seen ventrally to the respective branchial arches (Fig. 5b). In stage 31 the lateral muscles of all arches continue to grow and differentiate without major changes relative to the previous stage (Fig. 5c), the intermandibularis and interhyoideus come into contact, and four coracobranchiales are now visible (Fig. 5d).
In stage 32 all muscles are further differentiated (Fig. 6) and the head now appears, in a ventral view, almost completely covered by muscles (Fig. 6c). The intermandibularis and interhyoideus reach the ventral midline. The coracobranchiales are completely covered by the constrictores branchiales, and staining of deeper muscles is not visible. The cucullaris spans the entire length dorsally to the branchial arches (Fig. 6a, b). The cartilages are not clearly distinguishable because they are almost translucent at this stage with this methodology, but there was a faint attachment of the cucullaris onto the caudal branchial arch IV. Immediately caudal to the last branchial arch, the fibers of this muscle extend lateroventrally towards the pectoral girdle (Fig. 6a, b).
We observed neither the deeper true branchial muscles (adductores branchiales, interbranchiales and interarcuales laterales) nor the epibranchial muscles (interpharyngobranchiales and subspinalis); in adult sharks these deeper muscles are superficially covered by the constrictores branchiales, and attach onto branchial arches  (Table 1). That these muscles/bundles could not be seen can be explained by either the fact that they are not yet developed/differentiated or – more likely taking into account that all the other, more superficial, muscles of the branchial region are already seen – the penetration of the antibody was not deep enough. In fact, based on our studies of muscle development in other fishes and in tetrapods (cited above), it is very likely that most, or all, of these muscles started to differentiate in the oldest shark specimen(s) analyzed by us.
Thus, in summary, the anlagen (primordia) of mandibular arch muscles appear after the anlagen of hyoid arch muscles. The first branchial muscle anlagen can be observed simultaneously with the anlagen of the hyoid arch muscles, with the staining fainting from anterior to posterior (Fig. 3d). Still, analyzing the detailed appearance of muscles we could observe that muscles develop following an anterior to posterior direction, from the hyoid to the branchial arches and from outside to inside, i.e., lateral muscles develop before ventral muscles (except within the mandibular arch), and superficial muscles develop before deep muscles. Most muscles develop from their region of origin to their region of insertion (compare for examples Figs. 3e, 4f, 5d, 6c for the development of the intermandibularis and interhyoideus), except for the ventral branchial muscles coracobranchiales and the hypobranchial muscles coracomandibularis and coracoarcualis, which develop from their region of insertion to their region of origin (compare Figs. 4b, f, 5b, d for coracomandibularis and Figs. 4f, 5b, d, 6c for coracoarcualis).
On the origin of the pectoral and pelvic musculatures
In chondrichthyan and osteichthyan fishes the pectoral appendages are invariably described as developing before the pelvic appendages, in contrast to the condition found in most tetrapods, where they develop relatively simultaneously [55, 56]. In S. canicula the pelvic fin indeed starts to develop later than the pectoral one: the first signs of a pectoral fin outgrowth were identified as early as stage 19, while pelvic fin development was only detected at stage 25 (Fig. 1). Moreover, as previously suggested , all events characterizing fish fin development, such as formation of a transient AER, conversion of this structure into an AEF, outgrowth and differentiation of endoskeleton elements, occur earlier in the pectoral fins than in the pelvic fins. Here we show that appendicular myogenesis also occurs earlier in the pectoral fins (stage 28) than in the pelvic fins (stage 29) in S. canicula. Interestingly, the formation of the abductor and adductor muscles in the pectoral fins occurred simultaneously with the formation of the preaxial muscle, the pterygialis cranialis (Table 1). All these muscles seem to have been present in the last common ancestor (LCA) of the crown-group Gnathostomata [6, 22, 57]. Therefore, the non-simultaneous development of pectoral and pelvic musculature, which is commonly observed in osteichthyan fishes, may reflect the ancestral developmental process in the gnathostome lineage.
In adult chondrichthyans the pectoral abductor and adductor and the pelvic adductor have superficial and deep bundles, but the pelvic abductor has instead proximal and distal bundles, demonstrating that the significant anatomical differences between the pectoral and pelvic appendages of sharks concern not only hard tissues, but also soft tissues such as muscles [22, 49, 58]. Our results demonstrate that during early developmental stages the muscles of the pectoral and pelvic fins are more similar to each other than in adulthood. While developing, there are mainly two major undivided muscles in each fin, abductor and adductor, except for a preaxial muscle present only in the pectoral fin (the pterygialis cranialis). Thus, during late development, most likely after hatching, pelvic muscles undergo further elaboration becoming rather distinct from pectoral muscles. Interestingly, while we could observe the formation of the abductor and adductor muscles in the pelvic fins up to stage 34, we could not detect the development of the protractor even in the oldest stages, which suggests that considerable development of the pelvic musculature occurs, in fact, after hatching.
Apart from the differences between the adult abductors of the pectoral and pelvic appendages, and between the time of appearance of the musculature of each of these appendages, there is another major difference between the musculature of these appendages in S. canicula: the presence of several muscles connecting the pectoral girdle to cranial elements, such as the coracomandibularis, coracoarcualis, coracobranchiales, and the cucullaris, which all develop from the head region to the pectoral girdle region and which have no corresponding muscles in the pelvic appendage. This latter difference stresses the point that there are major functional and evolutionary reasons for the spatial correlation of the pectoral girdle with the skull in early gnathostomes: the internal branchial chamber seems to restrict the development of the pectoral girdle more anteriorly, which forms a protection for the pericardial cavity and an insertion for the pectoral fins .
In fact, studies in chondrichthyans have shown that the formation of branchial arches in sharks and the tetrapod forelimb share strikingly similar developmental mechanisms [47, 48], somewhat consistent with the view that the branchial arches and the pectoral appendage might be highly related evolutionarily/developmentally see also . For example, sonic hedgehog (Shh) is crucial to establish the anteroposterior polarity in both the outgrowing fin−/limb-bud and the developing gill arch . Other studies analyzed the body wall formation in lampreys as compared to gnathostomes, which is relevant as the gnathostome paired appendages start as outgrowths of body wall somatopleure . The somatopleure is a tissue containing somatic lateral plate mesoderm and overlying ectoderm . Lampreys are cyclostomes, i.e., vertebrates without jaw and paired fins. Tulenko and colleagues  suggest that the somatopleure is eliminated in lampreys while the lateral plate mesoderm is separated from the ectoderm and isolated to the coelomic linings during myotome extension. One way to interpret those data is that the somatopleure may have originally persisted close to the gills, established a pectoral fin, and afterwards, spread posteriorly to the pelvic level . This model has similarities to variations of the gill arch hypothesis . Interestingly, a recent study identified a Tbx5 fin enhancer, CNS12, in the noncoding region downstream of Tbx5 locus . The enhancer CNS12 was suggested to have driven the reporter gene expression in the lateral plate mesoderm posterior to the heart – a region where vertebrates with pectoral appendages show an apomorphic Tbx5 expression pattern . In the cephalochordate amphioxus Tbx4/5 is expressed in the pharyngeal and posterior mesoderm together with cardiac genes and is relevant for the development of a noncentralized heart . Other marker genes for vertebrate head and trunk mesoderm are also expressed in overlapping domains in amphioxus dorsal mesoderm, what indicates that the mesoderm is not yet differentiated along the craniocaudal axis . These data thus support the hypothesis that the mesoderm of the posterior head region, the heart, and pectoral appendages might have originated from a common ancestral region. This scenario might be an example for deep homology, in which structures evolve by the modification of pre-existing genetic regulatory circuits established in early metazoans . In fact, it was recently shown that the pharyngeal (head) muscles and the myocardium are developmentally and evolutionary more linked to each other than previously thought and that the so-called cardiopharyngeal field was likely present in the last common ancestor or of Olfactores (tunicates + vertebrates) .
The observations regarding the development of the cucullaris in S. canicula reinforce the idea of an ancestral close association between the head and pectoral girdle musculature. We showed that the anlage of the cucullaris clearly appears in the dorsal region of the branchial arches, without any connection between it and the anterior somites in early development (Fig. 4e). This further supports the hypothesis that the cucullaris is a true branchial muscle, as defended by classical authors such as Edgeworth  and in more recent developmental and molecular works . Tbx1 mutant mice, for example, have no trapezius or sternocleidomastoideus, which are derivatives of the cucullaris, and no branchial muscles, while somite-derived limb muscles are unaffected . Further evidence was provided by a fate map study in Ambystoma mexicanum where it was shown that the lateral plate mesoderm contributes to posterior branchial arch levators and to the cucullaris, what led the authors to suggest that this mesoderm should be regarded as posterior cranial mesoderm .
There are further indications consistent with the idea that the musculature connecting the head to the pectoral girdle could have derived from, or co-opted, similar developmental mechanisms than those used by the musculature of the posterior pharyngeal arches. In fact, there are three groups of muscles that connect these appendages to head structures other than the cucullaris: the hypobranchial muscles coracomandibularis and coracoarcualis attach the pectoral girdle to the mandible and (mostly) to the ceratohyal, respectively, while the ventral branchial muscles coracobranchiales connect it to the branchial arches. The muscles coracomandibularis and coracoarcualis cannot be used to support a similarity between the pectoral and posterior pharyngeal arch musculatures, because they are hypobranchial muscles derived from somites, and not branchiomeric head muscles, as are most of the muscles that connect the posterior pharyngeal arches to other cranial structures. However, the presence of the true branchial muscles coracobranchiales connecting the pectoral girdle to the branchial arches, exactly as numerous branchial muscles connect the branchial arches to each other, might constitute an argument consistent with the idea of a deep association between the pectoral girdle musculature and the branchial arch musculature. Particularly because the pelvic musculature has of course no muscles at all connecting it to the head, and thus to any branchial arch. These data contradict the hypothesis that pectoral and pelvic appendages and associated soft tissues are strictly serial homologous because this does not refer merely to the different topological position of the pectoral vs. pelvic appendages. Instead, this refers to completely different types of tissues, derived from completely types of primordia, being part of each of these two types of appendages. That is, the pectoral appendage includes/is related to branchial muscles that are derived from the cardiopharyngeal field, while no such muscles are related/part of the pelvic appendage, which exclusively includes muscles derived from somites.
Authors have recently suggested that in some placoderm fossils the pectoral and pelvic appendages seem to be more similar than previously thought . However, these studies do not include reconstructions of appendicular soft tissues such as muscles, which are crucial to discuss the similarity vs. dissimilarity of the pelvic and pectoral appendages as a whole. Anatomical and developmental studies performed on extant animals considered to have retained plesiomorphic musculature of gnathostomes , like chondrichthyans, show several aspects of dissimilarity between these appendages [18, 22]. Thus, strict serial homology does not seem to explain the origin of all the tissues that constitute/attach onto the pectoral and pelvic appendages. Further information on the cephalic and appendicular musculature of basal gnathostome lineages, such as the placoderms, would be ideal to infer to which extent chondrichthyans are plesiomorphic for this specific trait. We favor a scenario in which the different components of the pectoral and pelvic appendages may have arisen from distinct evolutionary processes leading to the integration of homoplastic structures. Moreover, the morphological evolution of the pectoral and pelvic appendages may have been conditioned by the position where they develop along the body axis, which result in distinct muscular phenotypes, including the crucial difference of the strong musculature connecting the pectoral girdle and the cranium.
Myogenic progression during muscle development
Previous studies have suggested that various vertebrate groups share a temporal and spatial myogenic progression during the development of the cephalic muscles: from lateral/superficial to ventral/medial (outside-in), from origin to insertion, and from anterior to posterior [28, 32, 33]. Additionally, cephalic muscle differentiation seems to be tightly correlated with the development of cephalic cartilages, which was formerly described by various authors [68,69,70,71]. However, it was not previously addressed whether a similar myogenic progression is also detected in chondrichthyans. Our data reveal that, in S. canicula, the lateral muscles of one arch differentiate before the ventral muscles of the same arch. Thus, cephalic muscle development occurs following a lateral to ventral myogenic progression, which resemble the process described in osteichthyans such as zebrafish, lungfish, amphibians, and birds [28,29,30,31,32,33, 70, 72]. This pattern is clearer in the branchial arches than in the mandibular and hyoid arches, with the mandibular arch being the only exception: the ventral intermandibularis develops before the other muscles of the first arch, which are more lateral.
In both the head and paired appendages of S. canicula, muscles normally develop from their region of origin to their region of insertion, as was previously reported for the cephalic musculature of other osteichthyans [28, 29, 32, 33, 70]. The only exceptions, within the cephalic muscles analyzed by us, are the coracomandibularis, coracoarcualis and the coracobranchiales, which developed in the head region from their adult region of insertion (mandible, ceratohyal, and branchial arches, respectively) and then extend posteriorly during development towards their adult region of origin (pectoral girdle). Only a few other exceptions to this origin-insertion myogenic progression were formerly described . Muscles with attachments on these cartilages remain without other attachments until the formation of the cartilages that lie in the adult region of origin of these muscles (e.g., otic capsule, pterygoid bone). This indicates that head muscle development depends on the underlying skeletal development , and this is probably why we see such a pattern in the coracomandibularis, coracoarcualis and coracobranchiales of S. canicula, as the coracoid develops later than Meckel’s cartilage, ceratohyal, and the branchial cartilages.
As most previous studies describing an anterior to posterior myogenic progression of the cephalic muscles of various non-chondrichthyan taxa (Table 2) differ in their methodology, we used fiber development as a criterion to access and compare order of development, and we grouped all muscles of the same pharyngeal arch into a single group. By doing this, one can consistently compare the data obtained for each taxon, and detect their developmental progression. All embryonic/larval amphibians shown in Table 2 develop their mandibular and hyoid arch muscles simultaneously and in most species (13 out of 20) these muscles also develop simultaneously with the first branchial arch muscles. In the amniote groups Aves and Theria the mandibular arch muscles clearly develop earlier than the hyoid arch muscles, which even develop later than the muscles of branchial arch I in Theria (Table 2). In S. canicula the mandibular muscles, in contrast, develop after the hyoid muscles, while within the hyoid and branchial muscles the normal anteroposterior myogenic progression of muscle differentiation takes place. Furthermore, other branchial muscles develop following an anterior to posterior myogenic progression with the muscles associated to the last arch developing latest.
However, one should note that in other fishes there are also exceptions to the anteroposterior myogenic progression (Table 2), which makes it difficult to infer whether this pattern is even the most commonly found in non-tetrapod vertebrates. For instance, two studies of the Australian lungfish (Neoceratodus forsteri) found minor differences in the developmental pattern of cephalic muscles [32, 73] and, while other developmental studies of lungfishes exist, none of them mentions the timing to clarify this pattern. In the zebrafish, the mandibular arch muscles develop before the hyoid arch muscles  and a recent study of the Longnose Gar (Lepisosteus osseus; Actinopterygii) describes a simultaneous development of mandibular and hyoid muscles . The Polypterus senegalus belongs to the Polypteriformes, which is the most basal extant actinopterygian family , and was also described as developing the hyoid muscles before other cranial muscles .
What can be inferred from the developmental studies on gnathostome muscle development, summarized here, is that the hyoid arch muscles develop and differentiate before the branchial arch muscles in non-amniote vertebrates, as also described in the results presented here. The order of appearance of the mandibular arch muscles in vertebrates seems to be more variable (Table 2). Unfortunately, no study of agnathans explicitly states the order of development of each cephalic muscle, which is required in the future, to investigate which pattern is plesiomorphic, and to discuss its implications for our understanding of the evolution of the musculature in vertebrates and gnathostomes.
Associations between ontogeny and phylogeny
Our previous works have indicated that in zebrafish and salamanders there is generally a parallelism between the order in which each cephalic muscle develops and the order in which each muscle was acquired during evolution (‘phylo-ontogenetic’ parallelism), barring only a few exceptions [28, 34]. A major problem with inferring a parallelism between the developmental order of appearance of muscles in sharks and the order in which the muscles appeared in evolution is that most of the muscles found in sharks and other gnathostomes are not present in any non-gnathostome extant taxon. Furthermore, there are insufficient detailed muscle reconstructions in fossils representing the transitions from agnathans to gnathostomes, making it difficult to infer the evolutionary order of appearance of the shark muscles.
However, given the phylogenetic position of sharks and their plesiomorphic muscular structure among gnathostomes , our observation that mandibular muscles develop after hyoid muscles implies that this might have been the ancestral process in the LCA of the crown-group Gnathostomata. In line with this idea, the simultaneous development of these two muscular units observed in osteichthyans may reflect derivation from the ancestral process. Interestingly, Miyashita  recently proposed that the ancestral mandibular arch was distinct from the pharyngeal arches, and only became secondarily similar to those structures during the evolutionary process that culminated with the origin of gnathostomes. Thus, mandibular and hyoid structures may have arisen from independent developmental processes, which then converge becoming increasingly similar over time. The patterns of cephalic muscle development observed in S. canicula, and particularly the fact that the mandibular muscles develop later than those of more posterior arches, breaking the seemingly stable anteroposterior myogenic progression seen in these latter arches in most osteichthyan clades, can thus provide important insights for further studies on the origin and early evolution of the gnathostome jaws.
1. Our dissections and analysis of muscles in S. canicula are consistent with idea that there is an anatomical/functional association between the musculature associated with the pectoral girdle and that associated with the posterior branchial arches in the crown-group Gnathostomata. This contradicts the view that the pectoral and pelvic appendages are strict serial homologues in these animals. Instead, we favor a scenario in which the pectoral girdle musculature may have arisen from a non-homologous process to the one involved in the origin of the pelvic musculature.
2. In both the head and paired appendages of S. canicula, muscles normally develop from their region of origin to their region of insertion. The only exceptions within all the cephalic muscles are the coracomandibularis, coracoarcualis, and the coracobranchiales, which develop from their adult region of insertion (mandible, ceratohyal, and branchial arches, respectively), and then extend posteriorly towards their adult region of origin (pectoral girdle). Furthermore, during cephalic muscle development, a lateral to ventral pattern can be observed, with the mandibular arch being the only one where there is an exception with a ventral muscle developing before the lateral ones. In S. canicula the mandibular arch muscles develop later than the hyoid muscles, while among the hyoid and branchial muscles one can observe an anteroposterior myogenic progression. Even with the exceptions described here, cranial muscle development appears to be highly conserved in gnathostomes.
3. In the chondrichthyan species analyzed here, the mandibular muscles develop later than the hyoid muscles as was also described for P. senegalus , which is a member of the most basal extant actinopterygian group Polypteriformes . In contrast, in most osteichthyans the mandibular muscles develop at the same time, or even earlier, then the hyoid muscles. A parallelism between ontogeny and phylogeny could be established if future studies provide further evidence consistent with Miyashita’s recent idea  that the mandibular arch was originally not integrated with or was not similar to the ancestral pharyngeal arches, and only became secondarily integrated with/similar to them in the transitions that lead to the LCA of crown-group Gnathostomata.
Mallatt J. The origin of the vertebrate jaw: neoclassical ideas versus newer, development-based ideas. Zoo Sci. 2008;25:990–8.
Coates MI. Palaeontology: beyond the age of fishes. Nature. 2009;458:413–4.
Janvier P. Early vertebrates: Oxford University Press; 1996.
Brazeau MD. The braincase and jaws of a Devonian ‘acanthodian’and modern gnathostome origins. Nature. 2009;457:305–8.
Cole NJ, Currie PD. Insights from sharks: evolutionary and developmental models of fin development. Dev Dyn. 2007;236:2421–31.
Diogo R, Johnston P, Molnar JL, Esteve-Altava B. Characteristic tetrapod musculoskeletal limb phenotype emerged more than 400 MYA in basal lobe-finned fishes. Sci Rep. 2016;6:37592.
Johanson Z. Placoderm branchial and hypobranchial muscles and origins in jawed vertebrates. J Vert Paleo. 2003;23:735–49.
Carr RK, Johanson Z, Ritchie A. The phyllolepid placoderm Cowralepis mclachlani: insights into the evolution of feeding mechanisms in jawed vertebrates. J Morph. 2009;270:775–804.
Freitas R, Zhang G, Cohn MJ. Biphasic Hoxd gene expression in shark paired fins reveals an ancient origin of the distal limb domain. PLoS One. 2007;2:e754.
Freitas R, Zhang G, Cohn MJ. Evidence that mechanisms of fin development evolved in the midline of early vertebrates. Nature. 2006;422:1033–7.
Tanaka M, Münsterberg A, Anderson WG, Prescott AR, Hazon N, Tickle C. Fin development in a cartilaginous fish and the origin of vertebrate limbs. Nature. 2002;416:527–31.
Neyt C, Jagla K, Thisse C, Thisse B, Haines L, Currie P. Evolutionary origins of vertebrate appendicular muscle. Nature. 2000;408:82–6.
Yonei-Tamura S, Abe G, Tanaka Y, Anno H, Noro M, Ide H, et al. Competent stripes for diverse positions of limbs/fins in gnathostome embryos. Evol Dev. 2008;10:737–45.
Kuratani S. Evolutionary developmental studies of cyclostomes and the origin of the vertebrate neck. Dev Growth Diff. 2008;50:S189–94.
Kuratani S, Horigome N, Hirano S. Developmental morphology of the head mesoderm and reevaluation of segmental theories of the vertebrate head: evidence from embryos of an agnathan vertebrate, Lampetra japonica. Dev Biol. 1999;210:381–400.
Kuratani S, Nobusada Y, Horigome N, Shigetani Y. Embryology of the lamprey and evolution of the vertebrate jaw: insights from molecular and developmental perspectives. Phil Tr Royal Soc B. 2001;356:1615–32.
Kuratani S, Murakami Y, Nobusada Y, Kusakabe R, Hirona S. Developmental fate of the mandibular mesoderm in the lamprey, Lethenteron japonicum: comparative morphology and development of the Gnathostome jaw with special reference to the nature of the trabecula cranii. J Exp Zool Part B. 2004;302B:458–68.
Ziermann JM, Miyashita T, Diogo R. Cephalic muscles of cyclostomes (hagfishes and lampreys) and Chondrichthyes (sharks, rays and holocephalans): comparative anatomy and early evolution of the vertebrate head muscles. Zool J Lin Soc - London. 2014;172:771–802.
Cerny R, Cattell M, Sauka-Spengler T, Bronner-Fraser M, Yu F, Medeiros DM. Evidence for the prepattern/cooption model of vertebrate jaw evolution. PNAS. 2010;107:17262–7.
Medeiros DM, Crump JG. New perspectives on pharyngeal dorsoventral patterning in development and evolution of the vertebrate jaw. Dev Biol. 2012;371:121–35.
Diogo R, Abdala V. Muscles of vertebrates - comparative anatomy, evolution, homologies and development. New Hampshire: CRC Press; Science Publisher, Enfield; 2010.
Diogo R, Ziermann JM. Muscles of chondrichthyan paired appendages: comparison with osteichthyans, deconstruction of the fore–hindlimb serial homology dogma, and new insights on the evolution of the vertebrate neck. Anat Rec. 2015;298:513–30.
Edgeworth FH. The cranial muscles of vertebrates. London: Cambridge at the University Press; 1935.
Diogo R, Abdala V, Lonergan N, Wood BA. From fish to modern humans - comparative anatomy, homologies and evolution of the head and neck musculature. J Anat. 2008;213:391–424.
Diogo R, Abdala V, Aziz M, Lonergan N, Wood B. From fish to modern humans - comparative anatomy, homologies and evolution of the pectoral and forelimb musculature. J Anat. 2009;214:694–716.
Diogo R, Ziermann JM. Development, metamorphosis, morphology, and diversity: the evolution of chordate muscles and the origin of vertebrates. Dev Dyn. 2015;244:1046–57.
Cole NJ, Hall TE, Don EK, Berger S, Boisvert CA, Neyt C, et al. Development and evolution of the muscles of the pelvic fin. PLoS Biol. 2011;9:e1001168.
Ziermann JM, Diogo R. Cranial muscle development in the model organism Ambystoma mexicanum: implications for tetrapod and vertebrate comparative and evolutionary morphology and notes on ontogeny and phylogeny. Anat Rec. 2013;296:1031–48.
Ziermann JM, Diogo R. Cranial muscle development in frogs with different developmental modes: direct development vs. biphasic development. J Morph. 2014;275:398–413.
Ziermann JM, Mitgutsch C, Olsson L. Analyzing developmental sequences with Parsimov – a case study of cranial muscle development in anuran larvae. J Exp Zool Part B. 2014;322B:584–604.
Ziermann JM, Olsson L. Patterns of spatial and temporal cranial muscle development in the African clawed frog, Xenopus laevis (Anura: Pipidae). J Morph. 2007;268:791–804.
Ziermann JM. Evolutionäre Entwicklung larvaler Cranialmuskulatur der Anura und der Einfluss von Sequenzheterochronien, Biologisch-Pharmazeutischen Fakultät. Jena: Institut für Spezielle Zoologie und Evolutionsbiologie mit Phyletischem Museum, Friedrich Schiller University Jena; 2008. p. 347.
Ericsson R, Olsson L. Patterns of spatial and temporal visceral arch muscle development in the Mexican axolotl (Ambystoma mexicanum). J Morph. 2004;261:131–40.
Diogo R, Hinits Y, Hughes SM. Development of mandibular, hyoid and hypobranchial muscles in the zebrafish: homologies and evolution of these muscles within bony fishes and tetrapods. BMC Dev Biol. 2008;8:1–22.
Diogo R, Molnar JL, Smith TD. The anatomy and ontogeny of the head, neck, pectoral, and upper limb muscles of Lemur catta and Propithecus Coquereli (primates): discussion on the parallelism between ontogeny and phylogeny and implications for evolutionary and developmental biology. Anat Rec. 2014;297:1435–53.
Trinajstic K, Sanchez S, Dupret V, Tafforeau P, Long J, Young G, et al. Fossil musculature of the most primitive jawed vertebrates. Science. 2013;341:160–4.
Gegenbaur C. Elements of comparative anatomy, Macmillan and Company, 1878.
Vicq-d’Azyr F. Parallèle des os qui composent les extrémités. Mém Acad Sci. 1774;1774:519–57.
Oken L. Lehrbuch der Naturphilosophie, Schultheß, 1843.
Owen R. On the nature of limbs: a discourse delivered on Friday, February 9, at an evening meeting of the Royal Institution of great Britain, John Van Voorst, 1849.
Kerr JG. Text-book of embryology. Vol. II: vertebrata with the exception of mammalia. London: Macmillan and Co. Limited; 1919.
Coates MI. The origin of vertebrate limbs. Development. 1994;1994:169–80.
Ruvinsky I, Gibson-Brown JJ. Genetic and developmental bases of serial homology in vertebrate limb evolution. Development. 2000;127:5233–44.
Young NM, Hallgrimsson B. Serial homology and the evolution of mammalian limb covariation structure. Evolution. 2005;59:2691–704.
Abbasi AA. Evolution of vertebrate appendicular structures: insight from genetic and palaeontological data. Dev Dyn. 2011;240:1005–16.
Dahn RD, Davis MC, Pappano WN, Shubin NH. Sonic hedgehog function in chondrichthyan fins and the evolution of appendage patterning. Nature. 2007;445:311–4.
Gillis JA, Dahn RD, Shubin NH. Shared developmental mechanisms pattern the vertebrate gill arch and paired fin skeletons. PNAS. 2009;106:5720–4.
Gillis JA, Hall BK. A shared role for sonic hedgehog signalling in patterning chondrichthyan gill arch appendages and tetrapod limbs. Development. 2016;143:1313–7.
Diogo R, Linde-Medina M, Abdala V, Ashley-Ross MA. New, puzzling insights from comparative myological studies on the old and unsolved forelimb/hindlimb enigma. Biol Rev. 2013;88:196–214.
Diogo R, Ziermann JM. Development of fore- and hindlimb muscles in frogs: morphogenesis, homeotic transformations, digit reduction, and the forelimb-hindlimb enigma. J Exp Zool Part B. 2014;322B:86–105.
Diogo R, Molnar J. Comparative anatomy, evolution, and homologies of Tetrapod Hindlimb muscles, comparison with forelimb muscles, and deconstruction of the forelimb-Hindlimb serial homology hypothesis. Anat Rec. 2014;297:1047–75.
Ballard WW, Mellinger J, Lechenault H. A series of normal stages for development of Scyliorhinus canicula, the lesser spotted dogfish (Chondrichthyes: Scyliorhinidae). J Exp Zool. 1993;267:318–36.
Freitas R, Cohn MJ. Analysis of EphA4 in the lesser spotted catshark identifies a primitive gnathostome expression pattern and reveals co-option during evolution of shark-specific morphology. Dev Genes Evol. 2004;214:466–72.
de Jong IML, Colbert MW, Witte F, Richardson MK. Polymorphism in developmental timing: intraspecific heterochrony in a Lake Victoria cichlid. Evol Dev. 2009;11:625–35.
Bininda-Emonds ORP, Jeffery JE, Sánchez-Villagra MR, Hanken J, Colbert M, Pieau C, et al. Forelimb-hindlimb developmental timing across tetrapods. BMC Evol Biol. 2007;7:1–7. doi:10.1186/1471-2148-7-182.
Yamanoue Y, Setiamarga D, Matsuura K. Pelvic fins in teleosts: structure, function and evolution. J Fish Biol. 2010;77:1173–208.
Molnar J, Johnston P, Esteve-Altava B, Diogo R. Musculoskeletal anatomy of the pelvic fin in Polypterus and the phylogenetic distribution and homology of pre- and postaxial pelvic appendicular muscles. J Anat. 2017;230:532–41.
Coates MI, Cohn MJ. Fins, limbs, and tails: outgrowths and axial patterning in vertebrate evolution. BioEssays. 1998;20:371–81.
Coates MI. The evolution of paired fins. Theory Biosci. 2003;122:266–87.
Tulenko FJ, McCauley DW, MacKenzie EL, Mazan S, Kuratani S, Sugahara F, et al. Body wall development in lamprey and a new perspective on the origin of vertebrate paired fins. PNAS. 2013;110:11899–904.
Adachi N, Robinson M, Goolsbee A, Shubin NH. Regulatory evolution of Tbx5 and the origin of paired appendages. Proc Natl Acad Sci U S A. 2016;113(36):10115–20.
Pascual-Anaya J, Albuixech-Crespo B, Somorjai IML, Carmona R, Oisi Y, Álvarez S, et al. The evolutionary origins of chordate hematopoiesis and vertebrate endothelia. Dev Biol. 2013;375:182–92.
Shubin N, Tabin C, Carroll S. Deep homology and the origins of evolutionary novelty. Nature. 2009;457:818–23.
Diogo R, Kelly RG, Christiaen L, Levine M, Ziermann JM, Molnar JL, et al. A new heart for a new head in vertebrate cardiopharyngeal evolution. Nature. 2015;520:466–73.
Lescroart F, Hamou W, Francou A, Théveniau-Ruissy M, Kelly RG, Buckingham M. Clonal analysis reveals a common origin between nonsomite-derived neck muscles and heart myocardium. PNAS. 2015;112:1446–51.
Theis S, Patel K, Valasek P, Otto A, Pu Q, Harel I, et al. The occipital lateral plate mesoderm is a novel source for vertebrate neck musculature. Development. 2010;137:2961–71.
Sefton EM, Bhullar B-AS, Mohaddes Z, Hanken J. Evolution of the head-trunk interface in tetrapod vertebrates. elife. 2016;5:e09972.
Hacker A, Guthrie S. A distinct developmental program for the cranial paraxial mesoderm in the chick embryo. Development. 1998;125:3461–72.
Noden DM. The embryonic origins of avian cephalic and cervical muscles and associated connective tissues. Am J Anat. 1983;168:257–76.
Schilling TF, Kimmel CB. Musculoskeletal patterning in the pharyngeal segments of the zebrafish embryo. Development. 1997;124:2945–60.
Noden DM. The role of the neural crest in patterning of avian cranial skeletal, connective, and muscle tissues. Dev Biol. 1983;96:144–65.
McClearn D, Noden DM. Ontogeny of architectural complexity in embryonic quail visceral arch muscles. Am J Anat. 1988;183:277–93.
Ericsson R, Joss J, and Olsson L. Early head development in the Australian lungfish, Neoceratodus forsteri. In: Jørgensen JM, Joss J, editors. Enfield: The Biology of Lungfishes, Science publisher, CRC Press, Tailor and Francis; 2010. pp. 149ff.
Konstantinidis P, Warth P, Naumann B, Metscher B, Hilton EJ, Olsson L. The developmental pattern of the musculature associated with the mandibular and hyoid arches in the Longnose gar, Lepisosteus osseus (Actinopterygii, Ginglymodi, Lepisosteiformes). Copeia. 2015;103:920–32.
Inoue JG, Miya M, Tsukamoto K, Nishida M. Basal actinopterygian relationships: a mitogenomic perspective on the phylogeny of the “ancient fish”. Mol Phylogenet Evol. 2003;26:110–20.
Noda M, Miyake T, Okabe M. Development of cranial muscles in the actinopterygian fish Senegal bichir, Polypterus senegalus Cuvier. J Morph. 1829:2017.
Miyashita T. Fishing for jaws in early vertebrate evolution: a new hypothesis of mandibular confinement. Biol Rev. 2015; doi:10.1111/brv.12187.
Platt JB. The development of the cartilaginous skull and of the branchial and hypoglossal musculature in Necturus. Morphol Jb. 1898;25:377–463.
Schlosser G, Roth G. Evolution of nerve development in frogs I: the development of the peripheral nervous system in Discoglossus pictus (Discoglossidae). Brain Behav Evol. 1997;50:62–93.
Schlosser G, Roth G. Evolution of nerve development in frogs II: modified development of the peripheral nervous system in the direct-developing frog Eleutherodactylus coqui (Leptodactylidae). Brain Behav Evol. 1997;50:94–128.
Noden DM, Marcucio R, Borycki A-G, Emerson CP, JR. Differentiation of avian craniofacial muscles: 1. Patterns of early regulatory Gene expression and myosin heavy chain synthesis. Dev Dyn. 1999;216:96–112.
Smith KK. Development of craniofacial musculature in Monodelphis domestica (Marsupialia, Didelphidae). J Morph. 1994;222:149–73.
Kaufman MH, and Kaufman MH. The atlas of mouse development, Academic press San Diego. 1992.
The experiments were conducted by RF in the Martin J. Cohn Lab (University of Florida) and we are grateful for his contribution providing insight and expertise that greatly assisted the research. We also acknowledge Raul Guizzo, as well as two anonymous reviewers, for carefully reviewing a previous version of this paper, as well as numerous colleagues for discussions on the subjects presented here.
RF was funded by FCT – Fundação para a Ciência e a Tecnologia (FCT), Portugal. JMZ and RD were funded by the Howard University College of Medicine. Funding sources did not influence the design of the study, the collection, analysis, or interpretation of data, or the writing of the manuscript.
Availability of data and materials
The material used for this study will be stored in Martin J. Cohn’s lab (University of Florida; via RF) and is available there upon request. All data needed are included in the paper. Additional photographs from the whole mount specimens and their dissections are stored electronically and are available upon request from JMZ. SEM figures can be requested via RF.
RD and JMZ designed the study. RF conducted the experiments and JMZ dissected specimens in RD’s Lab. JMZ and RF analyzed the specimen regarding muscle development. RF provided Figs. 1 and 2, and JMZ Figs. 4, 5 and 6. JMZ, RF, and RD discussed the data and wrote the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
All authors agree on the submission and publication of this paper and its included figures and tables.
Ethics approval and consent to participate
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ziermann, J.M., Freitas, R. & Diogo, R. Muscle development in the shark Scyliorhinus canicula: implications for the evolution of the gnathostome head and paired appendage musculature. Front Zool 14, 31 (2017). https://0-doi-org.brum.beds.ac.uk/10.1186/s12983-017-0216-y