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Table 1 Summary statistics of the Illumina HiSeq 2000 H. euphorbiae transcriptome assembly and annotation

From: Functional characterization of the Hyles euphorbiae hawkmoth transcriptome reveals strong expression of phorbol ester detoxification and seasonal cold hardiness genes

Raw no. of sequenced reads

21,656,040

No. of reads after quality control

17,257,056

Total assembly length [bp]

14,027,954

Q20 [%]a

89.52

Proportion of Ns [%]

0.02

GC content [%]

39.30

Mean abundance [k-mers] (min – max)b

16.17 (3 – 165)

No. of transcripts

18,080

No. of singleton contigs

11,514

No. of isogroups

1445

No. of unigenes

16,082

Mean no. transcripts per isogroup (min – max)

2.38 (2 – 5)

Mean length of transcripts [bp] (min – max)

775.89 (200 – 5412)

N50c [bp]

1084

No. of transcripts with BLASTX hitsd

8289

No. of transcripts with GO annotationsd

7070

No. of transcripts with EC/KEGG annotationsd

2806

No. of GO annotated transcripts with tag hitsd, e

4451

  1. aPhred Quality Score
  2. bMean coverage of k-mers that support a k + 1 length (= 32 bp) of a transcript
  3. cminimal length of the set of transcripts that makes up half of the total assembly length
  4. dall BLASTX hits with e < 10− 5 and a sequence similarity of > 55%
  5. etranscripts that where a best BLASTN hit (bit score > 40) for DeepSuperSAGE tags