Skip to main content
Fig. 1 | Frontiers in Zoology

Fig. 1

From: Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats

Fig. 1

Comparison of MUP levels between males and females. a Concentrations of total urine proteins in males and females were assayed using the Bradford protein assay (**P < 0.01, n = 7, independent-sample t-test). b MUP levels in the urine (2 μL of diluted urine samples) from male and female Lewis rats (n = 7 for each sex) were detected using SDS-PAGE with a marker of molecular weight (10.5 kDa-175 kDa, CW0986S, Beijing ComWin Biotech Co., Ltd., China). One male sample was loaded on every gel as a standard to normalize the intensities of samples on different gels. The gel shown is a representative sample of four females and four males. c MUP abundance was quantified in SDS-PAGE gels using the ImageJ program, and the data were shown as the mean ± standard error (SE), n = 7 (**P < 0.01, independent-sample t-test). d Differences in MUP levels of urine (1 μL of a 1:10 dilution) between males and females (n = 7 for each sex) were assessed using IEF with a marker of isoelectric point (pI) in 1D gel (pI 5-6, 42,967, SERVA, Germany). The gel shown is a representative sample of four females and four males. The bands 1-3 represented the three most abundant MUPs

Back to article page