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Fig. 2 | Frontiers in Zoology

Fig. 2

From: Two predominant MUPs, OBP3 and MUP13, are male pheromones in rats

Fig. 2

MUP levels in males were regulated by androgen. a SDS-PAGE showed the amount of MUPs in sham-operated, castrated and T-treated male rats (the molecular weight of marker: 10.5 kDa-175 kDa, CW0986S, Beijing ComWin Biotech Co., Ltd., China). The gel shown has 12 urine samples from three groups (2 μL of 2-fold dilution for each lane); remaining samples were on another gel which is not shown. One sham-operated male urine sample was run as a standard on every gel, and its intensity was used to normalize all values. b The relative abundance of MUPs on SDS-PAGE gels was analysed using the ImageJ program (mean ± SE, n = 7, **P < 0.01, one-way ANOVA followed by Tukey’s post hoc HSD test). c Each MUP in the urine samples (1 μL of a 1 in 10 dilution) from different groups was detected using IEF with a marker of pI values in 1D gel. (pI 5-6, 42,967, SERVA, Germany. S: sham-operated, C: castrated, C + T: castrated + T-treated). The bands 1-3 were the three most abundant proteins in MUPs

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