Fig. 5
From: Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda)
Colocalisation of synapsin- with GABA-IR at body wall neuromuscular junctions. a-c: Confocal images (maximum projection of 18 images at 1 μm intervals) of ventral body wall muscle 2 h labeled with antibodies against GABA (a) and synapsin (b) displaying numerous co-labeled boutons (c). d-f: Fluorescence images of lateral body wall muscle 34 (a dorsoventral muscle not depicted on Fig. 1). Presynaptic sites appear as aggregates of several small synapsin-IR dots, some of which are co-labeled against GABA (f, white arrowheads), whereas most of them are not labeled for GABA (hollow arrowheads). Anterior is to the left in a-c, dorsal is to the left in d-f. Scale bar: a-c 100 μm, d-f 10 μm