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Fig. 6 | Frontiers in Zoology

Fig. 6

From: Neuromuscular transmitter candidates of a centipede (Lithobius forficatus, Chilopoda)

Fig. 6

Colocalisation of synapsin- (green) with glutamate-IR (red) at leg neuromuscular junctions. a Merged confocal images (maximum projection of 33 images at 1 μm intervals) of muscle 48a in the femur, displaying numerous co-labeled boutons (arrowheads). A motor nerve containing several fibers labeled for glutamate is indicated by arrows. b Merged confocal images (maximum projection of 25 images at 1 μm intervals) of muscle 43 in the trochanter 2, displaying numerous co-labeled boutons (arrowheads) and strong glutamate-immunoreactivity surrounding synapsin-IR motor endings, indicating presumptive glia cells (arrows). c Merged confocal images (maximum projection of 28 images at 1 μm intervals) of muscle 48d (claw flexor) in the femur displaying numerous co-labeled boutons (arrowheads). Presumptive glial cells surrounding synapsin-IR motor endings are strongly labeled for glutamate (arrow). d Merged fluorescent images of muscle 42c in the trochanter 2 displaying numerous co-labeled boutons (arrowheads). Numbers refer to muscle numbers. Distal is to the right in all panels. Scale bars: a 100 μm, b-d 50 μm

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