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Fig. 15 | Frontiers in Zoology

Fig. 15

From: Insights into the structure and morphogenesis of the giant basal spicule of the glass sponge Monorhaphis chuni

Fig. 15

Graphs showing results of NMR, XRD and FTIR analyses. a 13C CP-MAS ssNMR spectrum of PG recorded on a Bruker AV300 spectrometer using a 4 mm CP-MAS Bruker probe at RO = 11.962 Hz (MAS), SF = 75.51 MHz, RD = 1 s, tcp = 1 ms, collecting NS = 976.896 scans and using LB = 50 Hz for apodisation. b 29Si HPDec-MAS ssNMR spectra of AL (in blue) and PG (in green) recorded on a Bruker AV700 spectrometer using a 2.5 mm MAS Bruker probe at RO = 20 kHz (MAS), SF = 139.10 MHz, RD = 60 s at 30° pulse angle, collecting NS = 6.000 scans for sample AL (I) and 4.000 scans for PG spectrum, and using a line broadening LB = 100 Hz for the post-treatment. The spectral deconvolution using Dmfit software is shown for the AL underneath the spectra allowing calculation of Q2, Q3 and Q4 contributions. Spectra were normalized based on the Q4 component as the rotor could not be completely filled (approximately at two thirds), by lack of material. c 1H OnePulse MAS ssNMR spectra of AL (in blue) and PG (in green) recorded on a Bruker AV700 spectrometer using a 2.5 mm MAS Bruker probe at RO = 20 kHz, SF = 700.14 MHz, RD = 5 s with NS = 4 scans for both samples. For the purpose of a quantitative comparison of the spectral profiles, PG spectrum was drawn with a diminishing scaling factor (0.67), as the rotor of sample AL was filled only to 2/3. *: 2.5 BL probehead signal (systematically present, whatever the sample). d 1H MAS ssNMR spectra of glassy silica: Spin echo experiment (in blue) and OnePulse experiment (in green). Experiments were recorded on a Bruker AV700 spectrometer using a 2.5 mm MAS Bruker probe at RO = 20 kHz (MAS), SF = 700.14 MHz, RD = 5 s with NS = 16 scans for both. *2.5 BL probehead signal (systematically present, whatever the sample). e XRD powder diffractograms of AL (in black) and PG (in red). f FT-IR spectrum of the PG

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