Skip to main content
Fig. 10 | Frontiers in Zoology

Fig. 10

From: Eggs to long-legs: embryonic staging of the harvestman Phalangium opilio (Opiliones), an emerging model arachnid

Fig. 10

Stage 13 (A–C′). In situ hybridization for the gene Popi-engrailed (A–B′) and confocal micrographs of fluorescent antibody staining against tropomyosin (C, C′). A–B′: Seventh opisthosomal segment. C: Maximum projection of a flat-mounted embryo. C′: Detail of the chelicera and pedipalp (ventral view). A and P: anterior–posterior axis; arrowhead: stripe of engrailed expression marking the posterior border of opisthosomal segment; blue circle: Hoechst (blue, gray); white circle: bright field; yellow circle: tropomyosin (yellow); ch: cheliceral segment; lr: labrum; L1–4: leg 1–4 segments; pp: pedipalpal segment; ppe: pedipalpal endite. Scale bars A–B′: 100 µm. C: 250 µm. C′: 125 µm

Back to article page