Fig. 11

Stage 14 (A–C′′). Confocal micrographs of fluorescent antibody staining against tropomyosin (A, C–C′′) and alpha-tubulin (B). A: Maximum projection of flat-mounted embryo stained for tropomyosin and Hoechst (ventral view). Dotted lines mark the coxae of pedipalps and L1 legs. B: Maximum projection of mid-optical section of flat-mounted embryo stained for alpha-tubulin and Hoechst (ventral view). Anterior appendages have been dissected for clarity. C–C′′: Optical sections of the head region of the same preparation in A. Note the tubular pharynx musculature. A and P: anterior–posterior axis; blue circle: Hoechst (blue); yellow circle: tropomyosin (yellow); green circle: tropomyosin (green); ca: corona analis. ch: chelicera segment; cec: circumesophageal connective; cm: commissure; gn: ganglium; lr: labrum; L1–4: leg 1–4 segments; ph: pharynx; pp: pedipalp. Scale bars: 250 µm. B reprinted with permission from Sharma (2018) Current Biology 28, R774–R778