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Fig. 3 | Frontiers in Zoology

Fig. 3

From: Eggs to long-legs: embryonic staging of the harvestman Phalangium opilio (Opiliones), an emerging model arachnid

Fig. 3

Stage 4 (A–D′). Confocal micrographs of an embryo of P. opilio stained with Hoechst (nuclei). A: Z-stack maximum intensity projection of the whole embryo. B, C: Optical sections at 6 and 11 µm depths (outward to inward), respectively. Note that larger nuclei occur more internally to the small nuclei. D: Z-stack maximum intensity projection of surface of the embryo shown in “A” rotated 90° forward. D′: detail of the same image in D, showing a denser spot of small nuclei at the surface of the embryo (bracket). Arrowheads: larger sub-peripheral nuclei. Scale bar A–D′: 200 µm

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Frontiers in Zoology

ISSN: 1742-9994