Fig. 8

Stage 10 (A–C′). In situ hybridization for the gene Popi-engrailed (A–B′) and confocal micrographs of fluorescent antibody staining against tropomyosin (C, C′). A–B′: Fourth opisthosomal segment and ventral flexure of the opisthosoma. C: Maximum projection of a flat-mounted embryo (ventral view). C′: Detail of the head lobes of same preparation shown in C. Only Hoechst channel in shown (gray). A and P: anterior–posterior axis; arrowhead: stripe of engrailed expression marking the posterior border of opisthosomal segment; blue circle: Hoechst (blue, gray); white arrow: isolated tropomyosin-positive cell; white circle: bright field; yellow circle: tropomyosin (yellow); af: anterior furrow; ch: cheliceral segment; lr: labrum; lf: lateral furrow; L1–4: leg 1–4 segments; pp: pedipalpal segment; yk: yolk. Scale bars A–B′: 100 µm. C: 250 µm. C′: 125 µm