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Fig. 4 | Frontiers in Zoology

Fig. 4

From: Movement and storage of nematocysts across development in the nudibranch Berghia stephanieae (Valdés, 2005)

Fig. 4

Confocal images of early stage Berghia stephanieae juveniles. These images show development of the cnidosac and beginning of nematocyst sequestration: A pre-feeding juvenile, B juvenile with cnidosacs and small cerata buds, C juvenile with two rows of cerata, and D juvenile with three rows of cerata. Animals were stained with acetylated alpha tubulin for cilia (magenta), DAPI for nematocysts (cyan), and Phalloidin for muscle (yellow). Some additional autofluorescence can be seen in BD from the dinoflagellates in the gut of each animal. (A’D’ and A”D”) are close up images of the epithelium (A’ and A”) or a cnidosac (B’D’ and B”D”) from the whole animal confocal images, and (A”D”) only show the Phalloidin (yellow) channel to highlight the musculature of the cnidosacs. Abbreviations: ci, cilia; cs, cnidosac; d, dinoflagellates; m, muscle; nem, nematocyst(s)

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