Fig. 5
From: MiR-34b/c play a role in early sex differentiation of Amur sturgeon, Acipenser schrenckii
Sequences conservation analysis of miR-34b/c between different species and their targeted mRNA prediction and verification. A Sequences conservation analysis of miR-34b/c between different species. has, Homo sapiens; mmu, Mus musculus; dre, Danio rerio; ola, Oryzias latipes; asc, Acipenser schrenckii. B The relationship of miR-34b/c and the 3′UTR of ar mRNA was predicted with the RNAhybrid software. C The quality of plasmid extracted from ar-3′UTR-psiCHECK-2 luciferase vector was evaluated using gel electrophoresis. Marker 2000 was used. D The cultured HEK-293 T cells were co-infected with ar-3′UTR-psiCHECK-2 luciferase vectors and mimic of three experimental groups (miR-34b, miR-34c, and miR-34b + miR-34c) and the NC group (negative control), respectively. Scale bar = 50 μm. E The firefly/Renilla luciferase activity ratio were respectively counted from three experimental groups and the corresponding NC control groups. *Represents P < 0.05 with a statistically significant difference between the two groups