Fig. 4
From: Cloning and functional analysis of the molting gene CYP302A1 of Daphnia sinensis
qPCR results of ecdysis gene CYP302A1 and its downstream response gene in D. sinensis fed by 5% E. coli concentration. Note: E5-EGFP: 5% E. coli concentration containing L4440-EGFP; E5-DIB: 5% E. coli concentration containing L4440-DIB. *stands for P < 0.05; **stands for P < 0.01. EcR: ecdysone receptor; USP: Ultraspiracle; E75: ecdysone-induced protein 75, which is a nuclear hormone receptor; HR3: hormone receptor 3; FTZ-f1: Fushi-tarazu factor 1, which is an ecdysone-inducible transcription factor. All these genes respond to the regulation of 20-hydroxyecdysone