- Open Access
The complex simplicity of the brittle star nervous system
© The Author(s) 2018
- Received: 30 September 2017
- Accepted: 11 December 2017
- Published: 1 February 2018
Brittle stars (Ophiuroidea, Echinodermata) have been increasingly used in studies of animal behavior, locomotion, regeneration, physiology, and bioluminescence. The success of these studies directly depends on good working knowledge of the ophiuroid nervous system.
Here, we describe the arm nervous system at different levels of organization, including the microanatomy of the radial nerve cord and peripheral nerves, ultrastructure of the neural tissue, and localization of different cell types using specific antibody markers. We standardize the nomenclature of nerves and ganglia, and provide an anatomically accurate digital 3D model of the arm nervous system as a reference for future studies. Our results helped identify several general features characteristic to the adult echinoderm nervous system, including the extensive anatomical interconnections between the ectoneural and hyponeural components, neuroepithelial organization of the central nervous system, and the supporting scaffold of the neuroepithelium formed by radial glial cells. In addition, we provide further support to the notion that the echinoderm radial glia is a complex and diverse cell population. We also tested the suitability of a range of specific cell-type markers for studies of the brittle star nervous system and established that the radial glial cells are reliably labeled with the ERG1 antibodies, whereas the best neuronal markers are acetylated tubulin, ELAV, and synaptotagmin B. The transcription factor Brn1/2/4 – a marker of neuronal progenitors – is expressed not only in neurons, but also in a subpopulation of radial glia. For the first time, we describe putative ophiuroid proprioceptors associated with the hyponeural part of the central nervous system.
Together, our data help establish both the general principles of neural architecture common to the phylum Echinodermata and the specific ophiuroid features.
- Brittle star
- Nervous system
Brittle stars are emerging model organisms in modern biology. They have been increasingly used to address a wide range of fundamental questions, including post-traumatic regeneration of lost body appendages [1–3], organization and physiology of the mutable connective tissue , and bioluminescence . Brittle stars are also among the fastest-moving echinoderms capable of coordinated complex locomotory behaviors. The neurobiology of ophiuroid locomotion has been receiving attention in the contexts of body plan evolution, neurobiology, and robotics. Unlike many other members of the phylum, they do not use their numerous small podia for movement. Instead, they quickly propel their body over the substratum via rapid large-scale rowing-like movements of highly motile segmented body appendages called arms. The behavior of the five individual arms is centrally controlled to produce a true bilateral movement pattern [6, 7]. To achieve this level of coordination, the nervous system must be able to synchronize muscular activity between different segments within each individual arm, as well as large-scale movements across the five arms.
All the above phenomena are controlled by or depend on the nervous system. Nevertheless, the ophiuroid nervous system has never been comprehensively studied with modern techniques. The last and only complete microanatomical description dates back to the 19th century  and remains the best reference to date. The pioneering neurophysiological studies that were performed in 1970s and 1980s [9, 10] provided valuable initial insights into the role of one brittle star neuronal type – giant neurons. They established the role of these cells in propagation of neural impulses throughout the nervous system and in integration of sensory inputs to coordinate contraction and relaxation of the arm muscles. These early works however have never been followed up by more detailed and comprehensive studies of other cell types and of the overall structure and function of neural circuits. Most modern reports focus on individual aspects of echinoderm neurobiology (e.g., immunostaining with one or a few cell type markers, ultrastructure of certain regions of the nervous system). These isolated studies, although each valuable in itself, do not assemble together into a general cohesive picture. The scope of this paper is to provide a comprehensive view of the organization of the nervous system in the brittle star arm that can serve as a reference for future studies in the biology of ophiuroids and echinoderms in general. We use a synthesis of different techniques to characterize various aspect of the neural architecture. These experimental approaches include three-dimensional (3D) modeling, transmission electron microscopy, and immunostaining with a series of cell-type specific glial and neuronal markers coupled with laser scanning confocal microscopy.
Present an anatomically precise digital 3D model of the nervous system in an arm segment. We made an effort to trace the origin and targets of all major peripheral nerves and propose standardized terminology for them.
Provide a detailed description of the neurohistological organization of the radial nerve cord, peripheral nerves and ganglia. We also discuss both the features that ophiuroids share with other echinoderms and unique characteristics of the brittle star nervous system
Describe the immunoreactivity of the cells of the nervous tissue with cell type-specific antibody markers.
Together, our data help establish both the general principles of neural architecture common to the phylum Echinodermata and the features that are specific to the class Ophiuroidea. We also confirmed and expanded on earlier observations  of complex and molecularly heterogeneous organization of echinoderm glia. This study also describes for the first time putative proprioceptors embedded in the CNS.
Animal collection and maintenance
Adult Amphipholis kochii Lütken, 1872 were collected from Vostok Bay, Sea of Japan (Russia). Adult individuals of Ophioderma brevispinum Say, 1825 were purchased from Gulf Specimen Marine Laboratories, Inc. (Panacea, FL). The animals were kept in glass aquaria with aerated sea water.
For transmission electron microscopy (TEM), arms of A. kochii were fixed in 2.5% glutaraldehyde dissolved in 0.05 M cacodylate buffer (pH 7.6) for 24 h at 4 °C. After fixation, the specimens were rinsed in the same buffer and postfixed in 1% OsO4 in cacodylate buffer for 1 h. The tissue samples were then decalcified in several changes of a solution containing 1% ascorbic acid and 0.15 M NaCl, dehydrated in a graded series of ethanol and acetone and embedded in the Araldite epoxy resin. Sections were cut with glass knives on Ultracut E (Reichert, Vienna, Austria) and UC6 (Leica) ultratomes. Ultrathin (50 – 70 nm) sections were stained with aqueous uranyl acetate and lead citrate and then examined and photographed with a Zeiss EM 10 transmission electron microscope.
For scanning electron microscopy (SEM), specimens were fixed in 2.5% glutaraldehyde in 0.05 M cacodylate buffer at pH 7.6, dehydrated in ethanol followed by an acetone series, critical point dried, and then sputter coated with carbon and gold. Specimens were examined with a Jeol JSM-IC848 scanning electron microscope (JEOL Ltd., Tokyo, Japan).
3D surface reconstruction
Complete series of transverse semi-thin (0.8 μm) sections were cut from one arm segment. For this purpose, we used one of the Araldite-embedded tissue samples that were prepared for TEM (see above). The sections were collected on gelatin-coated slides, stained with 1% toluidine blue in 1% aqueous sodium borate and mounted in DPX (Fluka). Every sixth section in the series was photographed with a Jenamed 2 (Carl Zeiss Jena) light microscope equipped with a Leica DC 150 digital camera. Preliminary image processing (brightness and contrast adjustments, etc.) were performed using Adobe Photoshop CS2 software. The stack of digitized micrographs was then imported into the Amira 3.1.1 volume modeling and visualization software (Mercury Computer Systems, Inc., Chelmsford, MA, USA), which was used for stack alignment, segmentation, and generation of the initial 3D model. Final editing of the model was performed in Blender, an open-source 3D editor (https://www.blender.org). Rendered images were generated using Blender’s Cycles engine using 500 samples.
The original stack of images, saved as a video file, is available in Additional file 1. The final 3D model in various interactive and non-interactive (video) formats can be accessed in Additional files 2, 3, 4, 5, 6, 7, 8, 9, 10 and 11.
Antibodies used in this study
Goat anti-mouse Cy3
Jackson Immunoresearch (115-165-146)
Goat anti-rabbit Cy3
Jackson Immunoresearch (115-165-144)
Goat anti-rabbit FITC
ThermoFisher Scientific (65-6111)
Goat anti-rat FITC
Whole-mount staining was performed in a similar way with the following modifications. After the fixation and decalcification steps, the tissues were bleached in increasing concentrations of hydrogen peroxide (0.3%, 1%, and 3%) and then permeabilized by Proteinase K digestion (2.5 μg/ml, 15 min at room temperature). All wash buffers contained 0.5% Triton X-100. The incubation steps in the first and second antibodies were increased to 2 days each and performed at 4 °C.
Stacks of optical sections were taken using the Olympus confocal laser scanning microscope FV1000. Maximum intensity Z-projections were generated in the Fiji image processing software .
Unless indicated otherwise, images of whole-mount specimens and micrographs of longitudinal sections are oriented with the distal side to the right.
Anatomical organization of the nervous system in the arm
The radial nerve cord in brittle stars has clear metameric organization and corresponds to segmented arrangement of other components of the arm, including the skeleton, ligaments, muscles, and water-vascular system. At the level of each vertebral ossicle, both the ectoneural and hyponeural layers of the RNC are thickened to form ganglionic swellings (Fig. 1d, d’). In each segment, the radial nerve cords give off a complex system of peripheral nerves that innervate different metameric anatomical structures of the arm (Figs. 3 and 4). This peripheral nervous system is described below.
Ectoneural peripheral nerves and ganglia. At the level of the podia, the ectoneural part of the RNC gives off a pair of short and thick podial nerves (Figs. 3, 4a, b, e and 5a, j). Shortly after emerging from the RNC, the podial nerve forms a thick ring ganglion at the base of the podium. A sleeve-like extension of the podial ganglion surrounds the hydrocoelic lining of the podium and descends down to the distal tip. The lateral side of the podial ganglion further gives off thick spine nerves to each spine (Figs. 3, 4a, b and 5a, j). As they penetrate the lateral arm shields, these nerves pass through spine ganglia. The podial ganglia also give off small nerves that innervate tentacle scales (Fig. 5j).
Hyponeural peripheral nerves. The hyponeural part of the RNC gives off an extensive system of peripheral nerves (Figs. 3 and 4c, d). Although the hyponeural system by itself forms no purely hyponeural peripheral ganglia, it contributes to the formation of the mixed ganglia, known as juxtaligamental nodes  (see below). At the level of the ganglionic swellings of the RNC, immediately proximal to the podial nerves, a pair of large proximal muscle nerves ascend from the lateral regions of the hyponeural cord, enter the vertebral ossicle and arch towards the median surface of the aboral muscles attached to the proximal surface of the vertebra (Figs. 3, 4d, f and 5b–d). There, it fuses with a wide ribbon-like median hyponeural nerve (Figs. 3, 4d, f and 5d–g). This flat nerve runs between the median surface of the intervertebral muscles – both aboral and oral – and the intervertebral ligament and fuses with its oral margin with the hyponeural part of the RNC (Figs. 3, 4d and 5h, i). It gives off numerous short branches on its surface that innervate both the intervertebral muscles and the mutable collagenous tissue of the intervertebral ligament in the central region of the arm (Figs. 4d, f and 5d–g).
On either side of the arm, a horizontalintermuscularnerve connects the median hyponeural nerves to the lateral juxtaligamental node (see below). This nerve runs between the intervertebral and lateral ligaments in the space between the oral and aboral muscles (Figs. 3, 4c, d and 5f, g).
Immediately behind the point of origin of the proximal muscle nerves, the hyponeural part of the RNC gives off two pairs of lateral nerves that both ascend aborally along the lateral surface of the oral intervertebral muscle (Figs. 4c, d and 5c–e, g). One of them – the distal lateral nerve – innervates the lateral arm shield, whereas the second one – the proximal lateral nerve – directly connects the radial nerve cord to the lateral juxtaligamental node (see below) (Fig. 5c–g).
Mixed (ecto-/hyponeural) peripheral nerves and ganglia represent the third subdivision of the arm peripheral nervous system that cannot be classified as either ectoneural nor hyponeural, as these structures are formed by contribution of both. All components of the mixed peripheral nervous system are embedded into the outer wall of the arm coelom. The most prominent structures of this mixed system are the paired large lateral juxtaligamental nodes that lie one on each side of the arm, midway between the oral and aboral surfaces (Figs. 3, 4e and 5f, g). Each lateral node is formed by the fusion of three neural components: the lateral end of the horizontal intermuscular nerve, the aboral end of the proximal lateral nerve, and the distal margin of the podial ganglion (Figs. 3, 4c–e and 5f, g). The first two structures originate from the hyponeural system, whereas the podial ganglion is ectoneural (Figs. 4a, b and 5a, d–g, j). The lateral juxtaligamental nodes give off numerous short branching protrusions that enter the adjacent collagenous tissue of the lateral ligament (Fig. 5f, g). They also give rise to two pairs of larger nerves, both thin and wide, which run in opposite directions in the lateral wall of the arm coelom (Figs. 3, 4e, f and 5f, i). One pair – the aboral mixed nerves – ascend towards the aboral midline (Figs. 3 and 5f, i). The two oral mixed nerves, one on each side of the arm, descend towards the oral midline and fuse to form the oral juxtaligamental node, which innervates the oral ligament (Figs. 3 and 5h).
Cellular architecture of the nervous tissue
Radial nerve cord
The ectoneural neuroepithelium of the RNC contains prominent radial glial cells (Fig. 6). The brittle star radial glia is very similar to their counterparts previously described in starfish and sea cucumbers [12, 14, 17, 18]. These cells are robustly labeled by the ERG1 antibody (Figs. 15a, 16e, f, 17b, c and 18b, c”’), which was raised against a sea cucumber glial antigen . They stretch throughout the height of the neuroepithelium between the apical and basal surfaces (Figs. 6a, 16e, f and 17c) and show clear epithelial cell features. Their cell bodies are often located at the apical surface of the neuroepithelium (Figs. 6a and 7a, b) and give off a long basal process that crosses the underlying neuropil (Figs. 6a and 7a, c). The distal end of the glial process forms a flattened endfoot that attaches to the basal lamina by hemidesmosomes (Fig. 6c). The cell bodies of adjacent glial cells are connected by apicolateral junctional complexes composed of zonula adherens and septate junctions (Fig. 6b). A typical intracellular characteristic of radial glia is the presence of thick bundles of intermediate filaments which run along the long axis of the cell (Fig. 6c, d). The epineural canal contains a dense accumulation of fibrous extracellular material that forms a flat cuticle-like structure overlaying the apical surface of the ectoneural neuroepithelium (Fig. 6a, d). This cuticle varies in thickness and sometimes shows a mesh-like organization of interconnected layers. The apical surface of the radial glial cells is attached to the cuticle via hemidesmosome-like contacts (Fig. 6d).
Most neuronal cell bodies are localized to the subapical region of the ectoneural neuroepithelium beneath the layer of glial cell bodies (Fig. 7a). Some neuronal perikarya, however, reach the lumen of the epineural canal (Fig. 7b). These neuronal cells are flanked by radial glia and joined to them by intercellular junctions. The region of the neuroepithelium located between the apical layer of the neuronal and glial cell bodies and the basal lamina is occupied by an extensive neuropil (Figs. 6a and 7c – f’). The neuropil is composed of densely packed neuronal processes filling all the space between the basal processes of radial glia (Fig. 7c). By their size, the neuronal processes can be clearly classified into “regular” (measuring 130 nm – 600 nm across) and “giant” (up to ∼5 μm across) (Fig. 7c, d). Occasionally, the neuropil contains processes of neurosecretory-like cells (Fig. 7e) filled with large dense granules, which are morphologically identical to those described in juxtaligamental cells . Well-defined chemical synapses are often seen in the neuropil (Fig. 7f, f’).
The hyponeural part of the brittle star RNC is also associated with two non-neural anatomical components. The first one is the radial hemal lacuna, which is a local expansion of the otherwise thin extracellular space that separates the ectoneural and hyponeural parts of the RNC (Fig. 9a). The lumen of the lacuna, therefore, does not have epithelial lining, but is instead surrounded by the basal lamina of the hyponeural neuroepithelium. The second non-neural component of the hyponeural system are two compact bundles of muscle cells immersed into the oral wall of the hyponeural cord on either side of the midline (Fig. 9a, e, g). These muscle bundles run longitudinally throughout the length of the radial nerve cord.
Peripheral nerves and ganglia
All peripheral nerves in the arm are organized as densely packed bundles of neuronal processes. They either run as anatomically distinct neural tracts or enter the wall of the arm coelom and form a basiepithelial nerve plexus there. Specific details on individual components of the peripheral nervous system are provided below.
We used a number of cell-type specific markers to study localization of different cell types in the brittle star nervous system (Table 1). These include antibodies against general neuronal antigens, such as synaptotagmin B (SynB), ELAV, and acetylated tubulin; Brn1/2/4 – an antigen specific to neuronal progenitors, and the neuropeptide GFSKLYFamide [20–22]. To label glial cells, we used the ERG1 monoclonal antibody that stains radial glia in the echinoderm CNS .
The anti-GFSKLYFamide antibodies label two well-defined longitudinal nerve tracts in the ectoneural neuropil of the RNC (Fig. 16a, a’, d, d’, f, f’). These tracts are positioned on either side of the midline and run continuously throughout the length of the arm. More loosely organized longitudinal processes also run on either side of the longitudinal tracts, but they do not form well-defined bundles (Fig. 16a, a’). In each ganglionic swelling of the RNC, the midline region between the two longitudinal tracts also contains a network of fibers (Fig. 16a–c), which largely dissipates in interganglionic regions. In each arm segment, the longitudinal GFSKLYFamide-positive tracts give off side branches that contribute to the podial nerves and podial ganglion (Fig. 16d, d’). The immunopositive neuronal cell bodies are not very numerous and are scattered in the ectoneural neuroepithelium without forming any noticeable clusters (Fig. 16a–c, e, e’). They lie at the apical region of the neuroepithelium. Some are clearly bipolar with an apical process reaching towards the lumen of the epineural canal and the basal axon descending in to the neuropil. Staining with the anti-GFSKLYFamide antibodies also reveals that at least some neurons in the RNC occupy stereotyped positions in different arm segments. For example, we identified a pair of large ectoneural unipolar neurons, which were always precisely localized to the distal region of the ganglionic swelling and projected their axons into the longitudinal tracts in the ectoneural neuropil (Fig. 16b, c).
The neuron-specific RNA-binding protein ELAV is expressed in the majority of (or possibly all) neurons in both the ectoneural and hyponeural neuroepithelia of the RNC (Fig. 17). The anti-ELAV antibody also labels neurons in peripheral nerves, such as the podial nerve and the proximal muscle nerve (Fig. 17b, b’). The immunoreactivity appears to be specific to neurons, as ELAV-positive cells are never co-labeled by the radial glial marker ERG1 (Fig. 17d–d”’).
The transcription factor Brn1/2/4 is expressed in numerous cells of the ectoneural epithelium in the RNC. The expression is however restricted to cells of the ganglionic swelling (Fig. 18). Double immunostaining with the anti-Brn1/2/4/ antibody and the glial maker ERG1 shows that Brn1/2/4 is produced in both radial glial cells and neurons (Fig. 18c–c”’). Although all neurons (i.e., ERG1-negative cells) in the ganglionic swelling appear to express this transcription factor, there are two populations of glial cells: Brn1/2/4-positive glia and Brn1/2/4-negative glia. In the ganglionic swelling these two types of glia are intermixed, whereas all glia in the interganglionic regions appear to be Brn1/2/4-negative.
Acetylated tubulin is also a reliable marker of the peripheral nervous system, as it marks both large nerves and their finest branches (Fig. 20). For example, it strongly stains the lateral and oral juxtaligamental nodes and the processes that these ganglia give off to innervate the adjacent regions of the collagenous connective tissue (Fig. 20a, b). It also marks the hyponeural proximal muscle nerve throughout its course, including the fibers that contribute to the intermusclular nerve and those that join the median hyponeural nerve to innervate the intervertebral muscles (Fig. 20c). Other peripheral nerves strongly marked with acetylated tubulin include the ectoneural spine nerves (Fig. 20d).
In the recent years, there has been a renewed interest in revisiting the organization of the echinoderm CNS using modern techniques, including transmission electron microscopy and cell type-specific labeling to characterize individual glial and neuronal cell populations [14, 15, 18, 19, 23]. These recent studies resulted in a paradigm shift in our current understanding of echinoderm neurobiology and its phylogenetic significance. The echinoderm neural organization is no longer perceived as “enigmatic” or unusual and is now considered to share a number of key features with other deuterostomes, including chordates. First, the ectoneural and hyponeural parts of the CNS, which were previously considered anatomically and functionally separate structures [10, 24, 25], are now clearly established to be extensively crosslinked by direct neuronal connections [14, 19]. Moreover, these two components of the nervous system originate from the same source in development . Second, the neurons were found to extensively communicate via “classical” chemical synapses , which were previously considered to be absent in echinoderms. Another critical finding was that echinoderm CNS has neuroepithelial architecture with the scaffold composed of radial glial cells. These glial cells are similar to the chordate radial glia in a number of morphological and functional properties, including their function as neuronal progenitors in adult neurogenesis and neural generation [11, 12, 14, 17, 18, 27, 28].
All these new significant findings, however, mostly emerged from studies of the sea cucumber CNS, one of the five existing classes in the phylum Echinodermata. It is therefore unclear whether or not these newly discovered principles are applicable to other echinoderm classes and whether they characterize the nervous system of the phylum in general. In this study, we establish that all general features mentioned above (radial glia scaffold in the neuroepithelium, frequent chemical synapses in the neuropil regions in the neuroepithelium, direct anatomical connections between the ectoneural and hyponeural systems) are also seen in the nervous system of brittle stars. However, there are some distinct features too that are present in ophuiroids, but not in the sea cucumber CNS. One such characteristic is the clearly defined segmental organization of the arm nervous system at the anatomical and cellular levels, as has been suggested in some earlier studies [29, 30]. At the anatomical level, the RNC is subdivided into ganglionic swellings separated by narrower interganglionic regions. In each segment, the peripheral nerves emerge from the same regions of the RNC and innervate the same effectors. At the cellular level, one can identify the stereotypically positioned individual cell bodies of giant neurons that contribute their axons to certain areas of the neuropil. This observation has several implications: (1) an existence of a patterning mechanism responsible for the segmentation in development/arm regeneration; (2) a degree of functional autonomy of the nervous system within each segment.
Even though the nervous system of brittle stars shows a number of distinct features that set them apart from other echinoderms, the neuroanatomical design within the class Ophiuroidea appears remarkably conserved. For example, the arrangement of the peripheral nerves does not change across the three species studied so far, which represent three different ophiuroid families. Except for the minor differences, such as the number of spine nerves, the two species described in the present study – O. brevispinum and A. kochii – showed the same neuroanatomical architecture, which also matched Hamann’s detailed descriptions for Ophioglypha albida .
Another interesting feature of the brittle star nervous system is its intimate association with effectors, including the arm muscles and the collagenous connective tissue structures. The median hyponeural nerve is particularly remarkable in this regard, as it innervates both large intervertebral muscles and the adjacent intervertebral ligament. Not only does it give off numerous side branches penetrating the muscles, the nerve itself lacks glial covering and is not separated from the muscle by a basal lamina. This intimate association between the nervous system and effectors probably provides the anatomical basis for the distinct locomotory behavior of brittle stars. Unlike in other echinoderms, tube feet play a relatively minor role in the whole-body movement of brittle starts. Instead, they extensively use bending of their jointed arms to crawl over the substratum . These movements can be unusually rapid (by echinoderm standards) and are highly coordinated across the individual arms.
Direct motor control of the collagenous connective tissue is a unique echinoderm phenomenon . Neurosecretory-like cells, which in brittle stars are called juxtaligamental cells, have been consistently found in association with connective tissue structures capable of changing their mechanical properties. The reversible change has been reported to be involved in movement and posture control, while irreversible loss of tensile strength in ligaments and tendons is the main mechanism of CNS-controlled autotomy in echinoderms. The changes in the properties of the extracellular matrix are mediated by substances released by juxtaligamental cells. These neurosecretory cells, in turn, are believed to be directly innervated by the central nervous system. Here, we provide direct support to this model, as we consistently see direct chemical synapses formed by neuronal terminals on juxtaligamental cells. Previously, cell bodies of juxtaligamental cells were mainly found in peripheral ganglia (juxtaligamental nodes) in the direct vicinity of the mutable collagenous structures they control [16, 31]. Here, we found cells of identical ultrastructural appearance in the RNC. This suggests that there are two cohorts of juxtaligamental cells: peripheral juxtaligamental cells localized in the vicinity of the structures they control and the central juxtaligamental cells with the cell bodies localized in the RNC and processes contributed to peripheral nerves. The respective physiological roles of these two populations of neurosecretory cells remain to be established.
A pair of small bundles of muscles cells are also incorporated into the oral wall of the hyponeural part of the RNC and run throughout the length of the nerve cord. These bundles of myocytes within the RNC are unique features of the brittle star nervous system, as they have never been observed in other echinoderms. The purpose of these cells is not known, but their position and organization allows us to formulate some preliminary thoughts. These bundles are very small, especially in comparison with the powerful intervertebral muscles. The cytoskeletal components of their contractile apparatus are weakly developed. Finally, unlike other muscles in the arm, these myocytes are never connected to any of the skeletal elements in the arm. Instead, they are completely immersed into the nervous tissue and fully surrounded by glial and neuronal cells. Taken together, these three observations suggest that these bundles of myocytes are highly unlikely to generate any significant contractile force. We hypothesize that they instead may function as stretch receptors (proprioceptors) immersed into the CNS. It would be interesting to experimentally probe into the function of these cells and to trace their origin in development and regeneration.
Another important finding that emerged from this study is that it contributes evidence in support of the idea that the glia in echinoderms are diverse and heterogeneous. Previous studies of the sea cucumber CNS demonstrated that the radial glial cells in the RNC, in spite of being all morphologically alike, fell into two distinct subpopulations: some of the glia expressed the transcription factor Myc, while in others it remained transcriptionally silent . Here, we show that in the brittle star RNC, radial glial cells also differ in their expression of the Brn1/2/4 transcription factor and thus form distinct Brn1/2/4 + and Brn1/2/4 − subpopulations. These two glial subtypes are intermixed within the ganglionic swellings of the RNC, but the interganglionic regions contain only the Brn1/2/4 − glia. Besides glia, Brn1/2/4 is also expressed in all neurons within the ganglionic swellings and thus its expression is not turned off in fully mature brittle star neurons. Brn proteins are a subgroup of the POU family transcription factors. In vertebrates, they have been implicated in neurogenesis and specification of the neuronal fate [32, 33]. The neurogenic function appears to be evolutionary conserved, as Brn1/2/4 was expressed in post-mitotic differentiating neuronal progenitors in the developing larval nervous system of a sea urchin . The functional significance of Brn1/2/4 in a subset of glial cells remains unclear. One possibility is that this transcription factor marks the neurogenic population of radial glia. It has been previously shown that radial glia in sea cucumbers give rise to both neurons and new glial cells in neural regeneration, but also in the uninjured adult CNS [11, 12]. Even though neurogenesis is negligible in the brittle star RNC, radial glia undergoes rapid activation followed by extensive cell proliferation after arm autotomy (Mashanov et al., in preparation). It therefore remains to be established if the differences in gene expression is related to the potency of radial glial cells in post-traumatic neurogenesis.
An additional level of echinoderm glial complexity involves the fact that glial cells are not restricted to the CNS only. Here, we confirm the existence of the peripheral glial cells, previously reported by Byrne , which are associated with some peripheral nerves (e.g. the hyponeural proximal muscle nerve) and ganglia (e.g. spine ganglia).
Several brittle star species [3, 5, 6, 35, 36] are currently being developed as model organisms to address various biological questions, ranging from evolutionary biology to developmental and behavioral biology. Solid understanding of ophiuroid neurobiology is often required for the success of those various projects. In turn, the availability of cell-type specific markers is critical for studies of the organization and development of the nervous system. We, therefore, tested the suitability of a number of available antibodies (Table 1), both commercial and received as a gift from collaborators, for identification of specific cell populations in the central and peripheral nervous system in a brittle star. Acetylated tubulin, ELAV, and synaptotagmin B appear to be the best neuronal markers, whereas Brn1/2/4 also labels some, although not all, glial cells, as indicated above. The echinoderm glial marker ERG1  reliably marks radial glia in the O. brevispinum and also the peripheral glia associated with, e.g., the proximal muscle nerve.
Our results in combination with already available data on the sea cucumber nervous system enhance our understanding of general principles of echinoderm nervous system organization including:
The ectoneural and hyponeural components of the nervous system are extensively interconnected.
The CNS has neuroepithelial organization with the supporting scaffold formed by radial glial cells.
Radial glial cells in the CNS are molecularly and probably functionally diverse.
The brittle star CNS is highly metameric. The same pattern of peripheral nerves/ganglia and precisely positioned cell bodies of at least some neurons are repeated in all arm segments.
For the first time, we have described a system of putative proprioceptors that are associated with the CNS and embedded into the hyponeural neuroepithelium.
We tested the suitability of glial and neuronal markers for studies of the brittle star CNS. As expected, the radial glial cells reliably marked with the ERG1 antibody, whereas the best neuronal markers are acetylated tubulin, ELAV, and synaptotagmin B. The transcription factor Brn1/2/4, a marker of neuronal progenitors, is expressed in all neurons within the ganglionic swellings of the RNC, but also in a subset of glial cells.
The authors thank Dr. Robert Burke (University of Victoria, Canada) and Dr. José García-Arrarás (University of Puerto Rico) for their gift of the antibodies. We are also grateful to Ms. Beate Aschauer (LMU, Munich) for her technical assistance and to all members of the Mashanov lab at UNF for critical discussion and inspiring comments.
The study was supported by the Alexander von Humboldt Foundation and University of North Florida.
Availability of data and materials
All data are reported in the article and contained in additional files submitted along with the manuscript.
VM, OZ, and TH conceived the study. OZ and MK performed immunohistochemical analysis. OZ and VM collected light and electron microscopy data. OZ, VM, and DM reconstructed the 3D model of the nervous system. All authors analyzed and interpreted the data. VM drafted the manuscript. All authors edited the draft and prepared it for submission. All authors read and approved the final manuscript.
Ethics approval and consent to participate
No human subjects were involved in the study. All experiments with brittle stars were carried out in compliance with the NSF and NIH guideline. The brittle stars Amphipholis kochii and Ophioderma brevispinum are not regulated or endangered species.
Consent for publication
The authors declare that they have no competing interests.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Bannister R, McGonnell IM, Graham A, Thorndyke MC, Beesley PW. Coelomic expression of a novel bone morphogenetic protein in regenerating arms of the brittle star Amphiura filiformis. Dev Genes Evol. 2007; 218(1):33. https://doi.org/10.1007/s00427-007-0193-9.
- Purushothaman S, Saxena S, Meghah V, Swamy CVB, Ortega-Martinez O, Dupont S, Idris M. Transcriptomic and proteomic analyses of Amphiura filiformis arm tissue-undergoing regeneration. J Proteomics. 2015; 112:113–24. https://doi.org/10.1016/j.jprot.2014.08.011.
- Czarkwiani A, Ferrario C, Dylus DV, Sugni M, Oliveri P. Skeletal regeneration in the brittle star Amphiura filiformis. Front Zool. 2016; 13:18. https://doi.org/10.1186/s12983-016-0149-x.
- Wilkie IC. Functional morphology of the arm spine joint and adjacent structures of the brittlestar Ophiocomina nigra (Echinodermata: Ophiuroidea). PLoS ONE. 2016; 11(12):1–36. doi:10.1371/journal.pone.0167533.Google Scholar
- Delroisse J, Ullrich-Lüter E, Blaue S, Ortega-Martinez O, Eeckhaut I, Flammang P, Mallefet J. A puzzling homology: a brittle star using a putative cnidarian-type luciferase for bioluminescence. Open Biol. 2017;7(4). https://doi.org/10.1098/rsob.160300. http://rsob.royalsocietypublishing.org/content/7/4/160300.full.pdf.
- Astley HC. Getting around when you’re round: quantitative analysis of the locomotion of the blunt-spined brittle star, Ophiocoma echinata. J Exp Biol. 2012; 215(11):1923–9. https://doi.org/10.1242/jeb.068460. http://jeb.biologists.org/content/215/11/1923.full.pdf.
- Matsuzaka Y, Sato E, Kano T, Aonuma H, Ishiguro A. Non-centralized and functionally localized nervous system of ophiuroids: evidence from topical anesthetic experiments. Biol Open. 2017; 6(4):425–38. https://doi.org/10.1242/bio.019836. http://bio.biologists.org/content/6/4/425.full.pdf.
- Hamman O. Anatomie der ophiuren und crinoiden. Ztschrft f Naturw (Jena). 1889; 43:233–384.Google Scholar
- Brehm P. Electrophysiology and luminescence of an ophiuroid radial nerve. J Exp Biol. 1977; 71(1):213–27. http://jeb.biologists.org/content/71/1/213.full.pdf.
- Cobb JLS. Enigmas of Echinoderm Nervous Systems In: Anderson PAV, editor. Evolution of the First Nervous Systems. NATO ASI Series (Series A: Life Sciences), vol 188.Boston: Springer: 1989. p. 329–37.Google Scholar
- Mashanov VS, Zueva OR, García-Arrarás JE. Heterogeneous generation of new cells in the adult echinoderm nervous system. Front Neuroanat. 2015; 9:123. https://doi.org/10.3389/fnana.2015.00123.
- Mashanov VS, Zueva OR, García-Arrarás JE. Radial glial cells play a key role in echinoderm neural regeneration. BMC Biol. 2013; 11(1):49.View ArticlePubMedPubMed CentralGoogle Scholar
- Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B, Tinevez J-Y, White DJ, Hartenstein V, Eliceiri K, Tomancak P, Cardona A. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012; 9(7):676–82. https://doi.org/10.1038/nmeth.2019.
- Mashanov VS, Zueva O, Heinzeller T, Dolmatov I. Ultrastructure of the circumoral nerve ring and the radial nerve cords in holothurians (Echinodermata). Zoomorphology. 2006; 125(1):27–38. https://doi.org/10.1007/s00435-005-0010-9.
- Mashanov V, Zueva O, Rubilar T, Epherra L, García-Arrarás JE. In: Schmidt-Rhaesa A, Harzsch S, Purschke G, (eds).Structure and Evolution of Invertebrate Nervous Systems. Oxford and New York: Oxford University Press; 2016. Chap. 51 Echinodermata.Google Scholar
- Wilkie IC. The juxtaligamental cells of Ophiocomina nigra (Abildgaard) (Echinodermata: Ophiuroidea) and their possible role in mechano-effector function of collagenous tissue. Cell Tissue Res. 1979; 197(3):515–30. https://doi.org/10.1007/BF00233574.
- Viehweg J, Naumann WW, Olsson R. Secretory radial glia in the ectoneural system of the sea star Asterias rubens (Echinodermata). Acta Zool. 1998; 79(2):119–31. https://doi.org/10.1111/j.1463-6395.1998.tb01151.x.
- Mashanov VS, Zueva OR, Garcia-Arraras JE. Organization of glial cells in the adult sea cucumber central nervous system. Glia. 2010; 58(13):1581–93. https://doi.org/10.1002/glia.21031.
- Hoekstra LA, Moroz LL, Heyland A. Novel insights into the echinoderm nervous system from histaminergic and FMRFaminergic-like cells in the sea cucumber Leptosynapta clarki. PLoS ONE. 2012; 7(9):44220. https://doi.org/10.1371/journal.pone.0044220.
- Díaz-Miranda L, Blanco RE, García-Arrarás JE. Localization of the heptapeptide GFSKLYFamide in the sea cucumber Holothuria glaberrima (Echinodermata): a light and electron microscopic study. J Comp Neurol. 1995; 352(4):626–40. https://doi.org/10.1002/cne.903520410.
- Nakajima Y, Kaneko H, Murray G, Burke RD. Divergent patterns of neural development in larval echinoids and asteroids. Evol Dev. 2004; 6(2):95–104. https://doi.org/10.1111/j.1525-142X.2004.04011.x.
- Garner S, Zysk I, Byrne G, Kramer M, Moller D, Taylor V, Burke RD. Neurogenesis in sea urchin embryos and the diversity of deuterostome neurogenic mechanisms. Development. 2016; 143(2):286–97. https://doi.org/10.1242/dev.124503. http://dev.biologists.org/content/143/2/286.full.pdf.
- Díaz-Balzac CA, Lázaro-Peña MI, Vázquez-Figueroa LD, Díaz-Balzac RJ, García-Arrarás JE. Holothurian nervous system diversity revealed by neuroanatomical analysis. PLoS ONE. 2016; 11(3):1–22. https://doi.org/10.1371/journal.pone.0151129.
- Cobb JLS. Neurobiology of the Echinodermata In: Ali MA, editor. Nervous Systems in Invertebrates. Boston: Springer US: 1987. p. 483–525.Google Scholar
- Cobb JLS. The nervous systems of Echinodermata: Recent results and new approaches In: Breidbach O, Kutsch W, editors. The Nervous Systems of Invertebrates: An Evolutionary and Comparative Approach: With a Coda written by T.H. Bullock. Basel: Birkhäuser Basel: 1995. p. 407–24.Google Scholar
- Mashanov VS, Zueva OR, Heinzeller T, Aschauer B, Dolmatov IY. Developmental origin of the adult nervous system in a holothurian: an attempt to unravel the enigma of neurogenesis in echinoderms. Evol Dev. 2007; 9(3):244–56. https://doi.org/10.1111/j.1525-142X.2007.00157.x.
- Mashanov VS, Zueva OR, Heinzeller T, Aschauer B, Naumann WW, Grondona JM, Cifuentes M, Garcia-Arraras JE. The central nervous system of sea cucumbers (echinodermata: Holothuroidea) shows positive immunostaining for a chordate glial secretion. Front Zool. 2009; 6:11. https://doi.org/10.1186/1742-9994-6-11.
- Mashanov VS, Zueva OR, García-Arrarás JE. Myc regulates programmed cell death and radial glia dedifferentiation after neural injury in an echinoderm. BMC Dev Biol. 2015; 15(1):24. https://doi.org/10.1186/s12861-015-0071-z.
- Cobb JLS, Stubbs TR. The giant neurone system in ophiuroids. Cell Tissue Res. 1981; 219(1):197–207. https://doi.org/10.1007/BF00210028.
- Bremaeker ND, Deheyn D, Thorndyke MC, Baguet F, Mallefet J. Localization of S1– and S2–like immunoreactivity in the nervous system of the brittle star Amphipholis squamata (Delle Chiaje 1828). Proc R Soc Lond B Biol Sci. 1997; 264(1382):667–74. https://doi.org/10.1098/rspb.1997.0095. http://rspb.royalsocietypublishing.org/content/264/1382/667.full.pdf.
- Wilkie IC. Mutable collagenous tissue: overview and biotechnological perspective In: Matranga V, editor. Echinodermata. Berlin, Heidelberg: Springer-Verlag: 2005. p. 219–248.Google Scholar
- Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Südhof TC, Wernig M. Direct conversion of fibroblasts to functional neurons by defined factors. Nature. 2010; 463(7284):1035–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Brombin A, Grossier J-P, Heuzé A, Radev Z, Bourrat F, Joly J-S, Jamen F. Genome-wide analysis of the pou genes in medaka, focusing on expression in the optic tectum. Dev Dyn. 2011; 240(10):2354–63. https://doi.org/10.1002/dvdy.22727.
- Byrne M. Ophiuroidea. In: Microscopic Anatomy of Invertebrates. New York: Wiley-Liss: 1994. p. 247–343.Google Scholar
- Long KA, Nossa CW, Sewell MA, Putnam NH, Ryan JF. Low coverage sequencing of three echinoderm genomes: the brittle star Ophionereis fasciata, the sea star Patiriella regularis, and the sea cucumber Australostichopus mollis. GigaScience. 2016; 5(1):1–4. https://doi.org/10.1186/s13742-016-0125-6.
- Zandawala M, Moghul I, Yañez Guerra LA, Delroisse J, Abylkassimova N, Hugall AF, O’Hara TD, Elphick MR. Discovery of novel representatives of bilaterian neuropeptide families and reconstruction of neuropeptide precursor evolution in ophiuroid echinoderms. Open Biol. 2017;7(9). https://doi.org/10.1098/rsob.170129. http://rsob.royalsocietypublishing.org/content/7/9/170129.full.pdf.